Pentose synthesis in Escherichia coli

Mutants in Glucose Metabolism

“…Flux determination in the pentose-phosphate pathway of E. coli is not understood. Labeling experiments (168,169,172) suggest reduced use of the oxidative branch anaerobically, pgi mutants do not grow anaerobically on glucose (nor edd mutants on gluconate), and limitation in NADPH reoxidation may be an explanation. The idea that flux might be limited in the same way aerobically was not supported by a study of pyridine nucleotide levels in wild-type and in a mutant with elevated glucose-6-P dehydrogenase (173); also, loss of transhydrogenase made no difference to growth of a pgi mutant on glucose (174).…”

Utility and limitations of electron ionization and chemical ionization for the determination of position and extent of labeling for 18O- and 13C-containing permethylated alditols by gas chromatography/mass spectrometry

“…Of particular importance is the use of stable isotopes of sugars for studying carbohydrate metabolism (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20). Invaluable information regarding how labeled glucose is utilized when subjected to a variety of metabolic pathways is attainable.…”

Structure-retention relations in the gas chromatography of nucleosides

“…Uridine-5,6,7',2',3',4',5',5'-dg, cytid\ne-5,6,1' ,2' ,3' ,4' ,5' ,5'-dg, adenosine-2,8, , 2', 3',4', 5', 5'-dg, and guanosin e-l',2',3',4',5',5'-de were isolated from deuterated E. coli, strain B, mid-log harvest, which was grown on special order by Merck, Sharp & Dohme of Canada, Ltd., Montreal, Canada. From 500 mg of lyophilized cells, RNA and DNA were isolated by phenol extraction, following closely the procedure of Caprioli and Rittenberg(25). The mixed precipitate was incubated with deoxyribonuclease I (pH 7.2, 1 hour, 37°), to remove DNA.…”