Pentagamaboronon-0 Fructose Inhibited Migration and Overexpression of Matrix Metalloproteinases 9 on MCF-7/HER2 Breast Cancer Cells

Hairunisa, Indah; Utomo, Rohmad Yudi; Ertanto, Yogi; Jenie, Riris Istighfari; Meiyanto, Edy

doi:10.14499/indonesianjcanchemoprev9iss3pp134-142

Cited by 3 publications

(5 citation statements)

References 12 publications

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“…This showed that EEKP has promising anticancer potential. In addition, KP displayed a cytotoxic effect on HeLa, SKOV3, HuCCA-1, RMCCA-1, and T24 cancer cell lines [18,19,[21][22][23]. Given that the cytotoxic activity of KP in highly metastatic breast cancer cells has never been reported before, our findings are novel.…”

Section: Discussionmentioning

confidence: 83%

“…The MTT assay was carried out based on previous study with modifications on 4T1, MCF-7, and NIH-3T3 cell line [19]. 4T1 and MCF-7 cells are breast cancer cell models, whereas NIH-3T3 cells are noncancerous cell line models.…”

Section: Determination Of Cytotoxic Activity Using Mtt Assaymentioning

confidence: 99%

“…A total of 4 × 10 4 4T1 cells were distributed into 96 well plates, then incubated for 24 h at 37 • C, 5% CO 2 cells were washed using PBS 1× and given starvation medium (a culture medium with 0.5% FBS) and then incubated for 24 h. Then, each well was scratched vertically using a white tip and treated with Doxorubicin 0.5 µg/mL (positive control) and EEKP 16.25 µg/mL, 32.5 µg/mL, and 65 µg/mL. Finally, cells were observed under a light microscope (Olympus, Olympus Corporation, Japan) each time at 0 h, 8 h, and 24 h and captured by the camera [19].…”

Section: Determination Of Anti-migration Effect Using Scratch Wound H...mentioning

confidence: 99%

“…Several studies on this activity have been carried out. KP demonstrated antimetastatic activity in Human bile duct cancer (HuCCA-1 and RMCCA-1), ovarian cancer cells (SKOV3), and Cervical Cancer HeLa [19,20]. These three cancers have a high proclivity to spread.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic Activity, Anti-Migration and In Silico Study of Black Ginger (Kaempferia parviflora) Extract against Breast Cancer Cell

Hairunisa

Bakar

Da'i

et al. 2023

Cancers

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Metastatic breast cancer remains the leading cause of death in women worldwide. This condition necessitates extensive research to find an effective treatment, one of which is the natural medicine approach. Kaempferia parviflora (KP) is a plant believed to possess anticancer properties. Therefore, this study aims to determine KP’s bioactive compound, cytotoxic, and anti-migration activity in the highly metastatic breast cancer cell line model 4T1, also in the breast cancer cell model MCF-7 and noncancerous cell line NIH-3T3. Maceration with ethanol (EEKP) and infusion with distilled water (EWKP) was used for extraction. The MTT assay was used to test for cytotoxicity, and the scratch wound healing assay was used to test for the inhibition of migration. Phytochemical profiling of EEKP was performed using UHPLC-MS, and the results were studied for in silico molecular docking. Result showed that EEKP had a better cytotoxic activity than EWKP with an IC50 value of 128.33 µg/mL (24 h) and 115.09 µg/mL (48 h) on 4T1 cell line, and 138.43 µg/mL (24 h) and 124.81 µg/mL (48 h) on MCF-7 cell line. Meanwhile, no cytotoxic activity was observed at concentrations ranging from 3–250 µg/mL in NIH-3T3. EEKP also showed anti-migration activity in a concentration of 65 µg/mL. Mass Spectrophotometer (MS) structures from EEKP are 5-Hydroxy-7,4′-dimethoxyflavanone (HDMF), 5-Hydro-7,8,2′-trimethoxyflavanone (HTMF), Retusine, and Denbinobin. The in silico docking was investigated for receptors Bcl-2, Bcl-XL, ERK2, and FAK, as well as their activities. In silico result indicates that HTMF and denbinobin are bioactive compounds responsible for EEKP’s cytotoxic and anti-migration activity. These two compounds and standardized plant extract can be further studied as potential breast cancer treatment candidates.

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Section: Discussionmentioning

confidence: 83%

Section: Determination Of Cytotoxic Activity Using Mtt Assaymentioning

confidence: 99%

Section: Determination Of Anti-migration Effect Using Scratch Wound H...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytotoxic Activity, Anti-Migration and In Silico Study of Black Ginger (Kaempferia parviflora) Extract against Breast Cancer Cell

Hairunisa

Bakar

Da'i

et al. 2023

Cancers

Self Cite

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“…Irradiation of neutron beam to the boron compound causes the boron-10 atoms to split to Li nuclei and alpha radiation (He nuclei) which only give intracell effects within cancer cells [8,9,11,[31][32][33][34] as shown in Fig. 6 [35,36].…”

Section: Discussionmentioning

confidence: 99%

The Role of BNCT in Breast Cancer Treatment

et al. 2019

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Boron neutron capture therapy (BNCT) is a promising future technique of breast cancer therapy. BNCT is a cell-targetting therapy. In BNCT, the use of boron-10 combined with curcumin analog will provide selective radiation therapy only to breast cancer cells, whereas healthy cells will not be affected. This is because the alpha radiation from the boron-10 nuclei that fission due to thermal neutron irradiation only has a short range of between 4 and 9 μm, whereas the cell diameter is between 10 and 20 μm, so the reaction is limited only to the cancer cells. If the development of BNCT succeeds, then in the future the malignancy of the breast cancer will be effectively treated by cancer-cell targeted therapy, thereby avoiding the side effects of conventional therapy.

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Evaluating the Diverse Anticancer Effects of Laos Kaempferia parviflora (Black Ginger) on Human Melanoma Cell Lines

Park,

Shin,

Hong

et al. 2024

Medicina

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Cancer has become a consistent concern globally and increasingly fatal. Malignant melanoma is a rising concern, with its increased mortality. Kaempferia parviflora Wall. ex Baker (K. parviflora (KP)), commonly known as black ginger, is well known for its medicinal contributions. For the first time, in the following study we investigated the antimelanoma potential of Laos KP extracts in human cell lines. KP extracts (KPE) in methanol, DCM, and ethyl acetate showed strong cell inhibition in both melanomas, with KPE-DCM being particularly effective in inhibiting melanoma cell migration, invasion, and proliferation by inducing cell cycle arrest and apoptosis, while KPE-Hexane exhibited a low cell inhibition rate and a more limited effect. KPE affected the increased expression of caspase-3, PARP andBax and the decreased expression of the BcL-2, Mu-2-related death-inducing gene (MUDENG, MuD) protein. Furthermore, KPE enhanced apoptotic cells in the absence and presence of the pancaspase inhibitor Z-VAD-FMK. Interestingly, these apoptotic cells were significantly suppressed by the caspase inhibitor. Moreover, elevated mitochondrial membrane potential (MMP) and intracellular reactive oxygen species (ROS) levels, suggestive of KPE’s mitochondrial-mediated apoptosis in melanoma cells, were also confirmed. KPE treatment increased MMP levels, and upregulated the generation of ROS in A375 cells but not in A2058 cells. However, pretreatment with an ROS scavenger (NAC) suppressed KPE-induced cell death and ROS generation. These results clearly pointed out KPE-induced mitochondrial-mediated apoptotic cell death as the mechanism behind the inhibition of the human melanoma cells. Future studies exploring the role of specific ROS sources and their interaction with mitochondrial dynamics could deepen the existing understanding on KPE-induced apoptosis.

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Pentagamaboronon-0 Fructose Inhibited Migration and Overexpression of Matrix Metalloproteinases 9 on MCF-7/HER2 Breast Cancer Cells

Cited by 3 publications

References 12 publications

Cytotoxic Activity, Anti-Migration and In Silico Study of Black Ginger (Kaempferia parviflora) Extract against Breast Cancer Cell

Cytotoxic Activity, Anti-Migration and In Silico Study of Black Ginger (Kaempferia parviflora) Extract against Breast Cancer Cell

The Role of BNCT in Breast Cancer Treatment

Evaluating the Diverse Anticancer Effects of Laos Kaempferia parviflora (Black Ginger) on Human Melanoma Cell Lines

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