Pentadecanal inspired molecules as new anti-biofilm agents against <i>Staphylococcus epidermidis</i>

Ricciardelli, Annarita; Casillo, Angela; Papa, Rosanna; Monti, Daria Maria; Imbimbo, Paola; Vrenna, Gianluca; Artini, Marco; Selan, Laura; Corsaro, Maria Michela; Tutino, Maria; Parrilli, Ermenegilda

doi:10.1080/08927014.2018.1544246

Cited by 20 publications

(16 citation statements)

References 31 publications

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“…Our previously reported studies showed antimicrobial and anti-biofilm activities expressed by different marine cold-adapted bacteria isolated from polar regions, especially Antarctica, against staphylococci and P. aeruginosa [ 20 , 28 , 35 , 36 , 37 ]. Herein, we demonstrated that the cell-free supernatant of an Antarctic bacterium belonging to the Psychrobacter genus, named TAE2020, strongly affected some specific virulence features of P. aeruginosa isolates from CF patients, without affecting bacterial vitality or inhibiting bacterial growth.…”

Section: Discussionmentioning

confidence: 99%

Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients

et al. 2021

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Pseudomonas aeruginosa is an opportunistic pathogen often involved in airway infections of cystic fibrosis (CF) patients. Its pathogenicity is related to several virulence factors, such as biofilm formation, motility and production of toxins and proteases. The expression of these virulence factors is controlled by quorum sensing (QS). Thus, QS inhibition is considered a novel strategy for the development of antipathogenic compounds acting on specific bacterial virulence programs without affecting bacterial vitality. In this context, cold-adapted marine bacteria living in polar regions represent an untapped reservoir of biodiversity endowed with an interesting chemical repertoire. In this paper, we investigated the biological activity of a supernatant derived from a novel Antarctic bacterium (SN_TAE2020) against specific virulence factors produced by P. aeruginosa strains isolated from FC patients. Our results clearly show a reduction in pyocyanin and protease production in the presence of SN_TAE2020. Finally, SN_TAE2020 was also able to strongly affect swarming and swimming motility for almost all tested strains. Furthermore, the effect of SN_TAE2020 was investigated on biofilm growth and texture, captured by SEM analysis. In consideration of the novel results obtained on clinical strains, polar bacteria might represent potential candidates for the discovery of new compounds limiting P. aeruginosa virulence in CF patients.

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Section: Discussionmentioning

confidence: 99%

Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients

et al. 2021

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“…Also, the non-bactericidal nature of a drug is purported to reduce the probability of drug resistance and mutant development (Patsilinakos et al, 2019). In light of the aforesaid fact, several non-bactericidal antibiofilm molecules from natural sources have been reported recently against S. epidermidis (Artini et al, 2017; Ricciardelli et al, 2018; Patsilinakos et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Umbelliferone Impedes Biofilm Formation and Virulence of Methicillin-Resistant Staphylococcus epidermidis via Impairment of Initial Attachment and Intercellular Adhesion

Swetha

Pooranachithra

Ganapathy

et al. 2019

Front. Cell. Infect. Microbiol.

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Staphylococcus epidermidis is an opportunistic human pathogen, which is involved in numerous nosocomial and implant associated infections. Biofilm formation is one of the prime virulence factors of S. epidermidis that supports its colonization on biotic and abiotic surfaces. The global dissemination of three lineages of S. epidermidis superbugs highlights its clinical significance and the imperative need to combat its pathogenicity. Thus, in the current study, the antibiofilm activity of umbelliferone (UMB), a natural product of the coumarin family, was assessed against methicillin-resistant S. epidermidis (MRSE). UMB exhibited significant antibiofilm activity (83%) at 500 μg/ml concentration without growth alteration. Microscopic analysis corroborated the antibiofilm potential of UMB and unveiled its potential to impair intercellular adhesion, which was reflected in auto-aggregation and solid phase adherence assays. Furthermore, real time PCR analysis revealed the reduced expression of adhesion encoding genes (icaD, atlE, aap, bhp, ebh, sdrG, and sdrF). Down regulation of agrA and reduced production of secreted hydrolases upon UMB treatment were speculated to hinder invasive lifestyle of MRSE. Additionally, UMB hindered slime synthesis and biofilm matrix components, which were believed to augment antibiotic susceptibility. In vivo assays using Caenorhabditis elegans divulged the non-toxic nature of UMB and validated the antibiofilm, antivirulence, and antiadherence properties of UMB observed in in vitro assays. Thus, UMB impairs MRSE biofilm by turning down the initial attachment and intercellular adhesion. Altogether, the obtained results suggest the potent antibiofilm activity of UMB and the feasibility of using it in clinical settings for combating S. epidermidis infections.

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“…Various studies report strategies to prevent biofilm formation on medical devices such as catheters, sutures, stents and bone cement [ 9 , 10 , 11 , 12 ]. Several researchers are involved in the design and evaluation of specific molecules with potential therapeutic use in chronic and biofilm-related infections [ 13 , 14 ]. In particular, antimicrobial peptides (AMPs), a small bioactive protein consisting of 12–50 amino acids, seem to have emerged as promising active agents against bacteria, viruses, fungi, and also as potential chemotherapeutic agents [ 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Biofilm Activity of the Fungal Phytotoxin Sphaeropsidin A against Clinical Isolates of Antibiotic-Resistant Bacteria

Roscetto

Masi²,

Esposito

et al. 2020

Toxins

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Many pathogens involved in human infection have rapidly increased their antibiotic resistance, reducing the effectiveness of therapies in recent decades. Most of them can form biofilms and effective drugs are not available to treat these formations. Natural products could represent an efficient solution in discovering and developing new drugs to overcome antimicrobial resistance and treat biofilm-related infections. In this study, 20 secondary metabolites produced by pathogenic fungi of forest plants and belonging to diverse classes of naturally occurring compounds were evaluated for the first time against clinical isolates of antibiotic-resistant Gram-negative and Gram-positive bacteria. epi-Epoformin, sphaeropsidone, and sphaeropsidin A showed antimicrobial activity on all test strains. In particular, sphaeropsidin A was effective at low concentrations with Minimum Inhibitory Concentration (MIC) values ranging from 6.25 μg/mL to 12.5 μg/mL against all reference and clinical test strains. Furthermore, sphaeropsidin A at sub-inhibitory concentrations decreased methicillin-resistant S. aureus (MRSA) and P. aeruginosa biofilm formation, as quantified by crystal violet staining. Interestingly, mixtures of sphaeropsidin A and epi-epoformin have shown antimicrobial synergistic effects with a concomitant reduction of cytotoxicity against human immortalized keratinocytes. Our data show that sphaeropsidin A and epi-epoformin possess promising antimicrobial properties.

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Pentadecanal inspired molecules as new anti-biofilm agents against Staphylococcus epidermidis

Cited by 20 publications

References 31 publications

Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients

Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients

Umbelliferone Impedes Biofilm Formation and Virulence of Methicillin-Resistant Staphylococcus epidermidis via Impairment of Initial Attachment and Intercellular Adhesion

Anti-Biofilm Activity of the Fungal Phytotoxin Sphaeropsidin A against Clinical Isolates of Antibiotic-Resistant Bacteria

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