Penicillins and Cephalosporins

Weck, A.L. de

doi:10.1007/978-3-642-69088-4_16

Cited by 54 publications

(33 citation statements)

References 323 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reports of limited success with skin testing as a di agnostic method for cephalosporin allergy [1], and the need to investigate adverse reactions to these drugs in a number of patients, prompted us to look for cepha losporin-reactive IgE antibodies in the sera of sensi tive subjects. The results of our investigations in de veloping and applying a suitable immunoassay and in employing it to study allergen cross-reactivity be tween cephalosporins and penicillins are described here.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Drugs as Allergens: An Immunoassay for Detecting IgE Antibodies to Cephalosporins

Harle

Baldo²

1990

Int Arch Allergy Immunol

View full text Add to dashboard Cite

A radioimmunoassay employing cephalothin linked to a solid phase has been developed for the detection of cephalosporin-reactive IgE antibodies. Direct binding and inhibition studies demonstrated allergenic cross-reactivity between cephalosporins and penicillins, and quantitative hapten inhibition experiments identified the 2-thiophene group, and particularly the attached methylene group, of cephalothin as an allergenic determinant.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Adverse reactions to cephalosporins are well re cognised [1] and there have been a number of reports of immediate type 1 hypersensitivity reactions follow ing the administration of compounds belonging to this valuable class of antibiotics [1 -5].…”

Section: Introductionmentioning

confidence: 99%

Drugs as Allergens: An Immunoassay for Detecting IgE Antibodies to Cephalosporins

Harle

Baldo²

1990

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…We have no answer to this question. However, it has been demon strated that IgE and IgG antibodies against benzylpenicilloyl (BPO) determinants in some penicillin de rivatives or against the acyl side chain (Rl) of some cephalosporine derivatives were produced in human cases [33][34][35]. There is no clear explanation for the mechanism of anti-BPO or anti-acyl side chain anti body formation.…”

Section: Discussionmentioning

confidence: 99%

Experimental Drug-Induced Allergic Nephritis Mediated by Antihapten Antibody

Joh¹,

Shibasaki²,

Azuma³

et al. 1989

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Experimental drug-induced allergic nephritis (DIAN) was mediated by an antihapten antibody. It has been postulated that DIAN is induced by cellular and humoral mechanisms. We tried to induce DIAN in mice, where the mechanism depends on humoral immunity. The first attempt was made in mice actively producing antibodies against cephem antibiotics, i.e. cephalothin (CET). Acute interstitial nephritis (AIN), morphologically similar to human disease, was obtained by injection of a CET-protein conjugate into the renal cortex in mice producing anti-CET-IgE and IgG antibodies. AIN could also be induced when normal mice, passively given anti-CET IgG antibody, received a subsequent intrarenal challenge with CET-protein conjugate. These preliminary results indicate that IgG antibody has an important role in the genesis of DIAN. Further experiments were performed with monoclonal antibodies directed against haptens instead of antibiotics in order to clarify the Ig isotype concerned. In mice passively given anti-Dansyl-IgG2a or anti-NP-IgG2a monoclonal antibodies, a challenge with an appropriate hapten-protein conjugate into the renal cortex resulted in AIN. However, transfer of anti-Dansyl-IgE or anti-DNP-IgE monoclonal antibodies, followed by challenge, did not induce AIN. In the experimental systems described, the involvement of a cellular immune mechanism is excluded. The results suggest that IgG, but not IgE antibody, is essential for the induction of DIAN by humoral immune mechanism.

show abstract

“…A wide variety of methods have been used to measure serum antibodies directed against penicillin (de Weck, 1983) including the red celllinked antigen-antiglobulin reaction , haemagglutination (Thiel et al, 1964;Levine et al, 1966;Spath et al, 1971), radioimmunoassay (Lee et al, 1986), radioallergosorbent test (Kraft & Wide, 1976) However, because criteria for positive identification of drug-specific antibody activity are often poorly defined, it is difficult to determine objectively the prevalence of antibodies in patient and control groups in previous studies.…”

Section: Discussionmentioning

confidence: 99%

“…Such adverse reactions A wide variation in the prevalence of antimay be manifested in almost any of the clinical penicillin antibodies has been reported. For forms of hypersensitivity (de Weck, 1983); anti-example, Levine et al (1966) detected IgM antibodies of IgG, IgM and IgE classes are suspected benzylpenicilloyl (BPO, the major antigenic of mediating penicillin-induced haemolytic determinant formed from benzylpenicillin; Levine & Ovary, 1961) antibodies in 97% (16% IgG + ve) of a group of patients who had received no penicillin in the preceding 2 years, and in 100% of a group of patients (39% IgG + ve) who had received recent penicillin therapy. More recently, the prevalence of IgG anti-BPO antibodies in patients following high dose penicillin therapy has been variously reported to be 33% (de Haan & Kalsbeek, 1983), 38% (Adkinson & Wheeler, 1983) and 46% (Lee et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

A survey of the prevalence of penicillin‐specific IgG, IgM and IgE antibodies detected by ELISA and defined by hapten inhibition, in patients with suspected penicillin allergy and in healthy volunteers.

Christie

Coleman

Newby

et al. 1988

Brit J Clinical Pharma

View full text Add to dashboard Cite

1. IgG, IgM and IgE anti‐benzylpenicilloyl (BPO) antibody activities were determined by enzyme‐linked immunosorbent assay (ELISA) in sera from 100 patients who claimed to be allergic to penicillin, and from 50 healthy volunteers. Continuous frequency distributions for all three classes of anti‐BPO antibody, defined as differential binding (delta OD) to BPO‐human serum albumin (HSA) and HSA, were obtained for both groups. 2. For IgM and IgE classes the anti‐BPO activities were slightly but statistically significantly higher in the patient group compared with the volunteer group. 3. Hapten inhibition ELISAs were performed to confirm specificity for the BPO determinant. On the basis of antibody activities (delta OD values) greater than or equal to 0.3 and 50% inhibition of binding in the presence of 100 micrograms ml‐1 BPO‐caproate, BPO‐specific IgG antibody was identified in 4/100 of the patients' sera and in 1/50 of the volunteers' sera; BPO‐specific IgM was identified in 7/100 patients' sera and 1/50 volunteers' sera; and BPO‐specific IgE in 5/100 patients' sera and 1/50 volunteers' sera. 4. Not all sera with differential antibody binding to BPO‐HSA/HSA were inhibited by the BPO hapten. Hence, hapten inhibition assays are essential for the unambiguous demonstration of drug specific antibodies.

show abstract

Penicillins and Cephalosporins

Cited by 54 publications

References 323 publications

Drugs as Allergens: An Immunoassay for Detecting IgE Antibodies to Cephalosporins

Drugs as Allergens: An Immunoassay for Detecting IgE Antibodies to Cephalosporins

Experimental Drug-Induced Allergic Nephritis Mediated by Antihapten Antibody

A survey of the prevalence of penicillin‐specific IgG, IgM and IgE antibodies detected by ELISA and defined by hapten inhibition, in patients with suspected penicillin allergy and in healthy volunteers.

Contact Info

Product

Resources

About