Penicillin Susceptibility and Macrolide‐Lincosamide‐Streptogramin B Resistance in Group B <i>Streptococcus</i> Isolates from a Canadian Hospital

Sherman, Kathleen; Whitehead, Sue; Blondel-Hill, Edith; Wagner, Ken; Cheeptham, Naowarat

doi:10.1155/2012/540127

Cited by 6 publications

(3 citation statements)

References 15 publications

(9 reference statements)

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“…At present, the resistance rate of S. agalactiae to erythromycin in mastitis was as high as 94.1% from some parts of China, while India was 33.3% (Jain et al, 2012). Previous studies also found that the resistance rate of group B Streptococcus against erythromycin was also increasing by years in Canada and Taiwan (Sherman et al, 2012;Ko et al, 2001;Helena et al, 1997). The resistance rate of Streptococcus suis to macrolides was more than 50% (Martel et al, 2001), In Asia, such as China, Vietnam and Korea, clinical isolates of S. pneumoniae had resistance rates of over 70% to macrolides (Song et al, 2004;Sahm et al, 2008).…”

Section: Discussionmentioning

confidence: 92%

Correlation Analysis between the Antimicrobial Resistance and Virulence of Pathogenic Streptococcus Isolates from Cows

Ding¹

2023

PJZ

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Bovine Streptococcus are one of the main pathogens causing bacterial disease such as mastitis and endometritis in dairy farming. The virulence factors produced by Streptococcus are related to the occurrence of inflammation. To investigate the correlation between antimicrobial resistance and virulence traits of bovine Streptococcal isolates. Induced resistance was conducted for Streptococcus pneumonia ATCC49619 and erythromycin-sensitive strains by gradually increasing the antimicrobial concentration. Plasmid conjugation test was carried out by membrane filtration method. The correlation between antimicrobial resistance and virulence traits was analyzed by LD 50 and related genes. Sensitive Streptococcus isolates to erythromycin and S. pneumoniae ATCC49619 were induced to resistance in vitro, MIC value was from ≤ 0.5 μg/mL up to ≥ 64 μg/mL, and ermB or mefA resistant gene were carried. Transfer rate of resistance was 100% by plasmid conjugant, conjugants had obtained the resistance phenotype and the related resistance genes from the donor bacteria. The LD 50 of conjugants and induced resistance strains compared with parental strain, the virulence was lower than sensitive strains. The present study demonstrated that the virulence of resistant Streptococcus strains obtained by different drug resistance transfer methods was lower than that of their parents.

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Section: Discussionmentioning

confidence: 92%

Correlation Analysis between the Antimicrobial Resistance and Virulence of Pathogenic Streptococcus Isolates from Cows

Ding¹

2023

PJZ

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“…Erythromycin resistance was also identified in 1/3 of all GBS isolates and is no longer recommended for GBS IAP. One report from Canada in 2010-2011 found 26.6% of 158 GBS isolates resistant 38 while another study in upstate New York found resistance in 38.4% of 688 GBS isolates 39 . The bacteriostatic mechanism of antimicrobial action, incomplete placental transfer and unclear fetal/neonatal PK suggest that clindamycin may not provide the same level of protection from GBS infection as provided by beta-lactam antibiotic IAP, even with susceptible isolates.…”

Section: Clindamycinmentioning

confidence: 99%

Drugs for the Prevention and Treatment of Sepsis in the Newborn

Mukhopadhyay

Wade

Puopolo

2019

Clinics in Perinatology

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“…Streptococcus agalactiae infections are usually treated with penicillin. Nevertheless, with the presence of many penicillin-allergic individuals, macrolides and clindamycin remain important drug alternatives in these cases, and an increase in resistance to these has been described in several countries (Sherman et al, 2012;Lambiase et al, 2012;Kimura et al, 2013;Wang et al, 2013;Frö hlicher et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of glyceraldehyde-3-phosphate dehydrogenase fromStreptococcus agalactiaeNEM316

Nagarajan

Ponnuraj

2014

Acta Cryst Sect F

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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an essential enzyme involved in glycolysis. Despite lacking the secretory signal sequence, this cytosolic enzyme has been found localized at the surface of several bacteria and fungi. As a surface protein, GAPDH exhibits various adhesive functions, thereby facilitating colonization and invasion of host tissues. Streptococcus agalactiae, also known as group B streptococcus (GBS), binds onto the host using its surface adhesins and causes sepsis and pneumonia in neonates. GAPDH is one of the surface adhesins of GBS binding to human plasminogen and is a virulent factor associated with host colonization. Although the surfaceassociated GAPDH has been shown to bind to a variety of host extracellular matrix (ECM) molecules in various bacteria, the molecular mechanism underlying their interaction is not fully understood. To investigate this, structural studies on GAPDH of S. agalactiae were initiated. The gapC gene of S. agalactiae NEM316 encoding GAPDH protein was cloned into pET-28a vector, overexpressed in Escherichia coli BL21(DE3) cells and purified to homogeneity. The purified protein was crystallized using the hanging-drop vapour-diffusion method. The GAPDH crystals obtained in two different crystallization conditions diffracted to 2.8 and 2.6 Å resolution, belonging to two different space groups P2 1 and P2 1 2 1 2 1 , respectively. The structure was solved by molecular replacement and structure refinement is now in progress.

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Penicillin Susceptibility and Macrolide‐Lincosamide‐Streptogramin B Resistance in Group B Streptococcus Isolates from a Canadian Hospital

Cited by 6 publications

References 15 publications

Correlation Analysis between the Antimicrobial Resistance and Virulence of Pathogenic Streptococcus Isolates from Cows

Correlation Analysis between the Antimicrobial Resistance and Virulence of Pathogenic Streptococcus Isolates from Cows

Drugs for the Prevention and Treatment of Sepsis in the Newborn

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of glyceraldehyde-3-phosphate dehydrogenase fromStreptococcus agalactiaeNEM316

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