Penicillin selective enzyme electrode

Papariello, G. J.; Mukherji, Ajanta; Shearer, C. M.

doi:10.1021/ac60326a032

Cited by 92 publications

(28 citation statements)

References 18 publications

(3 reference statements)

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“…The enzyme based methods generally are more specific and more precise than the direct colorimetric procedures (1, 2). Recently, ion-selective membrane electrode based procedures were developed for many substrates (5)(6)(7)(8)(9)(10)(11)(12)(13)(14) including urea (5-7). Two approaches were proposed.…”

Section: Resultsmentioning

confidence: 99%

Determination of lead at the parts per billion level by cathodic stripping analysis

Kinard¹,

Propst²

1974

Anal. Chem.

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Cathodic stripping analysis of metals that form sparingly soluble oxides is a potentially powerful technique for the determination of trace metals in environmental samples. The technique is sensitive to monolayer amounts of deposit and selective because the metal must form a sparingly soluble oxide. Only a modest investment in equipment is required. The method has received little attention since the original suggestion of Brainina ( I ) .Recently, Miwa et al.(2) have reported a cathodic stripping method for lead a t the platinum electrode based on the electrolyte exchange technique. The method is applicable to samples containing 0.1 to 2 ppm lead with an error of *IO% a t the 1-ppm level.More recently, Young and Laitinen (3) have reported the cathodic stripping of iron in acetate media. The integrated stripping current a t constant deposition time was found to be a nonlinear function of concentration, which they attributed to the insulating properties of the ferric hydroxide film a t high coverages. Since the completion of this work, it has come to the authors' attention that Laitinen and Watkins ( 4 ) presented a paper a t the Houston meeting of the Electrochemical Society on the cathodic stripping of lead; although the results have not yet been published except in the form of an abstract of papers of the meeting.Studies in this laboratory of the electrochemical behavior of the transuranium elements a t the conducting glass electrode (CGE) showed that trace amounts of lead could be quantitatively deposited from dilute sulfuric acid and subsequently determined by cathodic stripping. This approach appeared to offer a 1000-fold increase in sensitivity over that of Miwa, et al. (2) without requiring an exchange of electrolyte solutions. The anodic depositioncathodic stripping of lead a t the CGE was examined, and a method was developed for the determination of lead in aqueous solution. The technique has been employed to determine lead in rainwater sampled a t intervals from various locations in South Carolina. EXPERIMENTALApparatus. The technique for preparing a conducting glass electrode (CGE) and the design of the electrolysis cell have been described ( 5 ) . A typical CGE has a cylindrical working area of -4 cm2 and a resistance of -25 ohms.The body of the electrolysis cell was cut from the lower part of a 250-ml polyethylene cylinder and fitted with a polyethylene cap drilled to accommodate the electrodes and a sparge tube. The helium sparge tube together with the isolated and reference electrodes were sheathed with heat shrinkable Teflon (Du Pont) tubing to minimize adsorption losses on the exposed borosilicate glass parts. The isolated electrode consisted of a spiral of platinum wire immersed in 0.01M sulfuric acid. A disk of porous Vycor (Corning Glass) silicate glass, attached a t the end of the isolated electrode tube and held in place by the Teflon sheath, served as the salt bridge between the isolated electrode and the solution in the electrolysis cell. All potentials were measured us. the mercury-mercuro...

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Section: Resultsmentioning

confidence: 99%

Determination of lead at the parts per billion level by cathodic stripping analysis

Kinard¹,

Propst²

1974

Anal. Chem.

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“…The enzyme can be immobilized on the outer surface or captured inside the membrane. Penicillinase was immobilized on the surface of a glass pH electrode and the enzymatically formed penicilloic acid changed the pH in the interface [183][184][185].…”

Section: Potentiometric Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors for Drug Analysis

Özkan

Kauffmann

Zuman

2015

Monographs in Electrochemistry

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“…Although the literature is quite replete with studies on β-lactamase-based enzymatic and microbial electrodes for detecting penicillin and its derivatives [17,19,20,[38][39][40], only a few reports concerning cephalosporin detection by such biosensors are available [18,21]. Matasumoto et al [18] constructed a cephalosporinase-based microbial flow-type sensor having Citrobacter freundii that showed good specificity towards cephalosporins but with limited detection range and response time.…”

Section: Substrate Specificity and Interference Due To Other Antibioticsmentioning

confidence: 99%

Cephalosporins Determination with a Novel Microbial Biosensor Based on Permeabilized Pseudomonas aeruginosa Whole Cells

Kumar

Kundu

Pakshirajan

et al. 2008

Appl Biochem Biotechnol

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A new potentiometric microbial biosensor based on Pseudomonas aeruginosa was developed in this study for detecting the cephalosporin group of antibiotics. Preliminary results with the biosensor indicated that P. aeruginosa cells, when treated with lysozyme, showed more efficiency in detecting cephalosporin C in a wide concentration range of 0.1-11 mM with high sensitivity compared to the normal cells. Optimization of the three important biosensor design parameters permeabilized cell contents, quantities of gelatin, and glutaraldehyde resulted in high performance of the biosensor. The optimized values of the above parameters were cell contents 2.5 mg/cm(2), gelatin 8.5 mg/cm(2), and 0.25% glutaraldehyde. The assay conditions, namely phosphate buffer pH, ionic strength, and temperature, were optimized for best performance of the biosensor. The specificity test of the biosensor towards detecting different beta-lactam antibiotics showed good response only for the cephalosporins. The operational and storage stability in detecting cephalosporin C indicated very good potential of the biosensor in detecting cephalosporins with high accuracy.

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Penicillin selective enzyme electrode

Cited by 92 publications

References 18 publications

Determination of lead at the parts per billion level by cathodic stripping analysis

Determination of lead at the parts per billion level by cathodic stripping analysis

Electrochemical Biosensors for Drug Analysis

Cephalosporins Determination with a Novel Microbial Biosensor Based on Permeabilized Pseudomonas aeruginosa Whole Cells

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