Watermelon is a horticultural plant liked by many people. Limited land and the conversion of optimal land use make the cultivation activities expand to marginal land. It will undoubtedly affect the quality of the watermelon produced. Mycorrhizal application in sub-optimum land is one way to improve soil conditions and reduce excessive lime and fertilizer inputs. Branch growth is also a determinant of the quality of watermelon production. Pruning as a way of managing branching is very necessary in the cultivation of watermelon. This study was conducted to investigate the interaction between mycorrhizal dose and pruning variations on the growth and yield of watermelon. The study was conducted using a factorial of 2 factors with 3 replications in a completely randomized design (CRD). The first factor was several mycorrhizal doses, namely 10 g/plant, 15 g/plant, and 20 g/plant. The second factor was the variation of pruning, which leaves 3 primary branches which are cut off at the 10th segment and secondary branches on the 1-10, and leaves 2 primary branches which are cut off at the 5th segment and secondary branches on the 11-20th segment. The results showed that there was an interaction between the application of mycorrhizal 15 g/plant with variations in pruning leaving 2 primary branches on the diameter of the watermelon stem, giving mycorrhizal significantly affected leaf area and fruit weight with the best results was obtain when mycorrhizal applied 20 g/plant, and pruning variations had an effect on on the diameter of the stems of watermelon plants with the best results obtained in variations of pruning leaving 3 primary branches.