Penggunaan Bakteri Kitinolitik sebagai Pengendali Hayati Colletotrichum capsici pada Tanaman Cabai

Abstract

Use of Chitinolytic Bacteria as Biological Control of Colletotrichum capsici on Chili PlantsColletotrichum capsici is known as the causal agent of anthracnose disease in chili plant and may cause reduction of crop yield. Chitinolytic bacteria, namely Serratia marcescens KAHN 15.12, Bacillus thuringiensis SAHA 12.12, and BAE 36 were reported to have antagonistic activity against C. capsici. Therefore, a study was conducted to determine the potential of chitinolytic bacteria on controlling C. capsici on chili plants in greenhouse experiment. Three bacterial isolates used as biocontrol agent was formulated by using talcum as carrier materials. The methodologies consisted of characterization of bacterial isolates, formulation of biocontrol agent, viability test of bacterial isolate, efficacy of biocontrol agents in the laboratory and in the greenhouse. Disease severity in the laboratory reached 64% when chili treated with isolate formulation of BAE 36. In the greenhouse, BAE 36 isolate formulation and consortium formulation were able to suppress infection of C. capsici; each was indicated by disease incidence of 25% and 50%, respectively. These results indicated that chitinolytic bacterial formulations could be potencial as biocontrol agents of C. capsici.

Characterization of chitinolytic bacteria isolated from Ipomea pes caprae

“…Chitinolytic bacteria that produce chitinase are beneficial in waste management, particularly waste containing chitin [13], as biocontrol agents and for medical and industrial purposes [17]. Chitinolytic bacteria can also be utilized as biological control against insect pests [3].…”

“…For the chitinolytic index, the clear zone created around the colony indicating chitinolytic activity was measured. The following equation was used to determine the chitinolytic index (IK) value [13]:…”

Potensi Asap Cair Tempurung Kelapa Sebagai Antijamur Fusarium Foetens, Fusarium Moniliforme, Dan Colletotrichum Capsici

“…Cabai merupakan tanaman hortikultura yang juga bernilai ekonomis tinggi di Indonesia, dengan peningkatan total produktivitas sebesar 3.76% pada tahun 2019. Produktivitas cabai merah dapat dipengaruhi oleh faktor hama, penyakit, atau iklim (Syam et al 2022). Penyakit utama yang menyerang tanaman cabai adalah penyakit antraknosa atau patek yang disebabkan oleh jamur Colletotrichum sp.…”

Exploration and Characterization of Chitinolytic Bacteria from Leachate, Shrimp Pond Water and Rhizosphere Roots

“…Pengujian aktivitas kitinase pada bakteri kitinolitik bertujuan untuk mengetahui kemampuan dari setiap isolat bakteri yang diperoleh dalam mengeluarkan enzim kitinase dan memastikan bahwa isolat bakteri hasil isolasi merupakan bakteri kitinolitik yang ditandai terbentuknya zona bening disekitar koloni bakteri. Aktivitas kitinase pada bakteri ditandai dengan perubahan warna indikator BCP 0,1% dari warna kuning menjadi ungu (Syahfitri et al, 2018). Pengamatan aktivitas kitinase dilakukan pada hari ke dua setelah pengujian dimana hasil menunjukkan adanya perubahan warna pada semua isolat bakteri hasil eksplorasi dari warna kuning menjadi ungu (Gambar 3).…”