Pengendalian Infeksi Bakteri Aeromonas hydrophila pada Ikan Nila (Oreochromis niloticus) dengan Ekstrak Rimpang Lengkuas Merah (Alpinia purpurata)

Abstract

MAS diseases (Motile Aeromonas Septicaemia) is caused by the bacterium Aeromonas hydrophila. One of the natural ingredients that can be used as an alternative to inhibit bacterial activity is the extract of red galangal rhizome (Alpinia purpurata). Active substances possessed of this material have potential as antibacterial. The purpose of this study is to determine the effectiveness of red galangal rhizome extract in the control of MAS disease in tilapia. The type of this research is experimental research in Completely Random Design (RAL) with 6 treatments, which are positive control, negative control, 250, 500, 750, and 1000 ppm. The result of the research on the activity of the galangal extract bacteria on A. hydrophila obtained the widest clear zone at 1000 ppm constipation, which is 9.7625 mm. The treatment has been conducted by immersion of Tilapia fish into the extracted red galangan rgizomes for 5 minutes, then it was put into aquarium for maintenance for 7 days. 0.1 ml A. hydrophila (108 cfu/ml) was injected to Tilapia. The result of the research on antibacterial shoes the wides clear zone at 1000 ppm with constipation, which is 9.7625 mm and the effectiveness test of red galangal extract on tilapia infected by A. hydrophila bacteria experience (ulcer) healing after 7 days with the concentration of rhizome extract of galangal 1000 ppm. Key words: red galangal rhizome extract, A. hydrophila, A. purpurata.

The The effectiveness of galangal (Alpinia galanga) extract for the treatment of sangkuriang catfish (Clarias gariepinus) infected Aeromonas hydrophila

“…Based on the LC50 24-hour test, a safe concentration for fish is below the LC50 value, which is below 760 mg/L. In the study by Sari et al (2017), which used galangal extract to treat Nile tilapia infected with A. hydrophila, the optimal dose was 1000 mg/L. When compared to the treatment of catfish infected with A. hydrophila, it can be considered more effective and efficient because at a concentration of 500 mg/L, it already resulted in the best survival rate of 90%.…”

“…Natural materials sourced from plants have been widely used to treat fish infected with Aeromonas hydrophila, including garlic and binahong leaves (Fitriyanti et al, 2020). Another natural material that can be used to treat fish infected with this disease is galangal (Sari et al, 2017). Galangal contains essential oils, phenols, and terpenoids that have bactericidal properties.…”

PENANGGULANGAN PENYAKIT BAKTERIAL PADA IKAN NILA MENGGUNAKAN EKSTRAK DAUN SIRIH (Piper betle L.) DI DESA GONTORAN LOMBOK BARAT

“…Tantu (2013), menyatakan bahwa penyakit ini menyebabkan kematian ikan budidaya diatas 80% dalam kurun waktu yang relatif singkat akibat dari tingkat keganasan Aeromonas hydrophila yang sangat tinggi. Menurut Sari et al (2013), bakteri Aeromonas hydrophila menggunakan sistem quorum sensing sebagai pengontrol virulensinya terhadap organisme lain. Aeromonas hydrophila yang bersifat virulen dapat dijadikan nonvirulen dengan cara menghambat sistem quorum sensingnya, sehingga sistem quorum sensing ini dapat dijadikan sebagai target untuk agen kemoterapeutik.…”

Optimasi vitamin C terhadap pertumbuhan dan kelangsungan hidup ikan nila (Oreochromis niloticus) yang diinfeksi Aeromonas hydrophila

“…Nilai derajat keasaman (pH) selama penelitian berkisar antara 7,08-8, meskipun telah dilakukan perlakuan dengan penambahan bakteri dan vitamin C nilai pH tetap stabil. Nilai pH dalam suatu perairan memiliki dampak besar terhadap kelangsungan hidup organisme di dalamnya (Sari et al, 2017). Perubahan nilai pH yang signifikan bisa memperlambat pertumbuhan hingga menyebabkan kematian ikan nila.…”