Pengenalan Teknik Pembuatan Benih Jagung Komposit Sinta Unram Di Desa Gumantar Kabupaten Lombok Utara

Sudika, I Wayan; Sutresna, I Wayan; Anugrahwati, Dwi Ratna; Aryana, I Gusti Putu Muliarta; Suliartini, Ni Wayan Sri

doi:10.29303/jsit.v2i2.48

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“…The attack symptoms shown by FAW larvae in this study had similar characteristics to the symptoms found by several previous ones [3,13,50,51]. Attacks can be initiated by the larvae punching holes in the young leaves of the plant and then punching holes in the young leaves that are still curled up to cut off the growing point of the corn plant.…”

Section: Discussionsupporting

confidence: 83%

Spatial Distribution and Genetic Diversity of The Fall Armyworm, <em>Spodoptera frugiperda</em> J.E. Smith (Lepidoptera: Noctuidae) in the Bali Province and Lesser Sunda, Indonesia

Supartha¹,

Susila²,

Yudha³

et al. 2022

Preprint

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Spodoptera frugiperda is an invasive pest that has spread in various parts of the world. These pests have the ability to spread and adapt highly to new habitats. Until now, it is not known with certainty the distribution of these invasive pests in Eastern Indonesia, especially Bali and Nusa Tenggara. This study aims to map the spatial distribution and genetic distribution of S. frugiperda which damages maize in the areas of Bali and Nusa Tenggara. This research was conducted using a survey method from May to September 2022 covering the islands of Bali, Lombok, Sumbawa, Sumba, Flores, and Timor. The results showed that S. frugiperda had spread evenly in Bali and Nusa Tenggara. The results of PCR amplification in the COI gene from 9 sample isolates from all research locations showed the similarity of DNA bands leading to the Spodoptera frugiperda species with a banding pattern length of 683 – 697. These results indicated that the distribution of genetic variants of corn caterpillars in Bali, NTB, and NTT was confirmed as S. frugiperda species. However, the isolated gene S. frugiferda, which was shown by the alignment results of the sequences from Lombok, was confirmed as a different strain from strains from Bali, Sumba, Sumbawa, Flores, and Timor. This incident can be seen from the difference in the protein base composition of S. frugiperda from Bali, Sumba, Sumbawa, Timor, and Flores. The results of phylogenetic analysis in this study confirmed 3 clusters of the genetic closeness of S. frugiperda. Cluster-1 comes from the results of the search for specimens of JB FAW and KB FAW from Bali, SB FAW and SB FAW Sorghum from Sumba, SW FAW from Sumbawa, KP FAW from Timor, and FL FAW from Flores. Cluster-2 is an isolate outside of our species. Cluster-3 comes from the search for LT and LS FAW specimens from Lombok. The genetic distance between cluster-1 and cluster-3 is quite far, which is 0.20 mu.

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Section: Discussionsupporting

confidence: 83%