Pengaruh Platelet-Rich Plasma (PRP) Terhadap Proliferasi dan Viabilitas Human Dermal Fibroblast (HDF) dalam Konsentrasi Glukosa Tinggi

Hadi, Restu Syamsul; Kusumah, Indra Maulana Yusup; Sandra, Yurika

doi:10.47349/jbi/15022019/213

Cited by 2 publications

(2 citation statements)

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“…PRP can increase cell viability in inflammatory conditions, proven by the CCk-8 results, which showed a maximum dose-dependent increase in cell viability at 10% PRP. This result supports previous research that found increased fibroblast cell viability with PRP administration (Hadi et al, 2019;Hassan et al, 2022). Fibroblasts are inactive under normal conditions; however, fibroblast cells will be active and carry out chemotactic growth factors when injury or inflammation occurs, which will cause angiogenesis.…”

Section: Discussionsupporting

confidence: 91%

Effect of platelet-rich plasma on fibroblasts induced by lipopolysaccharide: in vitro study for wound healing

Idris,

Kusuma,

Juniarti

2023

curr biomed

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Platelet-rich plasma (PRP) is expected to be an alternative therapy in wound healing by accelerating the inflammatory process and increasing the healing factors so that the healing process or inflammation is faster. The purpose of this study was to examine the effect of calcium chloride (CaCl2)-activated PRP on the viability, migration, and cytokine levels of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) of dermal fibroblast cells in lipopolysaccharide (LPS)-induced inflammatory conditions, as a model in wound healing in vitro. Fibroblast cells were grown in DMEM medium induced with LPS, and then CaCl2-activated PRP treatment was added. Measure fibroblast cell viability using CCK-8 kit (cell counting kit-8) was evaluated using a microplate reader, and the cell migration was evaluated using scratch-assay and TScratch software. Expression of IL-6 and VEGF using ELISA kit. All data were analyzed using software SPSS version 26 by performing a one-way analysis of variance (ANOVA), Kruskal Wills, and Mann-Whitney tests. The results showed that PRP significantly increased fibroblast cell viability in the 10% PRP treatment group. This study shows that PRP does not reduce IL-6 cytokine levels but can increase VEGF growth factor in fibroblast cell cultures. PRP increased cell migration so that the healing process was faster. In conclusion, the CaCl2-activated PRP on LPS-induced fibroblast cells can increase viability and accelerate cell migration; it can’t decrease IL-6 but can increase VEGF expression. PRP is expected to be an alternative therapy in wound healing.

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Section: Discussionsupporting

confidence: 91%

Effect of platelet-rich plasma on fibroblasts induced by lipopolysaccharide: in vitro study for wound healing

Idris,

Kusuma,

Juniarti

2023

curr biomed

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“…PRP can be directly applied in the inactive form because it will be activated after entering the body [9]. PRP can be stored at 20°C and can be used later [13].…”

Section: Platelet Rich Plasma (Prp)mentioning

confidence: 99%

Profile red blood cell of local cat during auto skin graft healing which are given platelet rich plasma (PRP) and treated with tilapia fish bio dressing

Abellisa,

Sriningsih,

Etriwati

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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The use of auto skin grafts is a solution to rehabilitate the anatomy and function of the wound area. This study aims to observe differences in the red blood profile of local cats using platelet-rich plasma (PRP) and Tilapia Mozambique skin bio-dressing during ASG healing. The study used 6 male domestic cats aged 1-2 years with a body weight of 2-4 kg which were divided into 2 treatment groups. The first surgical procedure, a 2x2 cm wound was created period of 4 days. The second surgical operation involved doing an ASG using a donor from the thoracic region to the area where the wound developed. In the first group (T-I) used bio-dressing, while treatment in the second group (T-II) used bio-dressing added PRP. Blood collection was carried out through the Cephalica antebrachii vein on days 0, 3, 6, 9 and 12 after surgery. The red blood profile including erythrocytes, hemoglobin, hematocrit, mean corpuscular volume (MCV) fluctuated not significantly (P>0.05) while mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and platelets fluctuated significantly. significantly different (P<0.05) between the treatment groups and the time of observation. As an alternate dressing and treatment that can aid in the healing of ASG, PRP and bio dressing can be applied.

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Pengaruh Platelet-Rich Plasma (PRP) Terhadap Proliferasi dan Viabilitas Human Dermal Fibroblast (HDF) dalam Konsentrasi Glukosa Tinggi

Cited by 2 publications

References 0 publications

Effect of platelet-rich plasma on fibroblasts induced by lipopolysaccharide: in vitro study for wound healing

Effect of platelet-rich plasma on fibroblasts induced by lipopolysaccharide: in vitro study for wound healing

Profile red blood cell of local cat during auto skin graft healing which are given platelet rich plasma (PRP) and treated with tilapia fish bio dressing

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