The study to examine an ability of zeolites on suppressing the growth of Coliform and Salmonella in the hatchery pellets during storage was conducted at the Feed Technology Laboratory, Faculty of Animal Agriculture Sciences, Diponegoro University. The zeolite has been reported has a special ability to absorb substances, exchange ions and as a catalyst. This zeolite was expected suppress the growth of pathogenic bacteria, thus elongated life time as well as maintained quality of the pellet. In the pelleting process, the hatchery waste was added with 0% and 3% zeolites, then the pellets were stored for 4, 8, and 12 weeks, with 3 replicates of each treatment combinations. Data were statistically analyzed by ANOVA. Results of experiments showed that addition of zeolite in the pelletizing hatchery waste do not influence the content of Coliform in the pellets during storaging and the Salmonella maintained to be negatives. However, the average number of Coliform bacteria remained on the critical category of safety standard of feedstuffs i.e. 10 5 cfu/g. It could be concluded that pelleting was effective suppressed growth of Coliform and Salmonella, but an addition of zeolite was not effective in inhibiting the growth of pathogen on the hatchery waste pellets during storage.