Pengaruh Penambahan Follicle Stimulatins Hormone (FSH) dan Pregnant Mare's Serum Gonadrotropin (PMSG) dalam Sel Granulosa Terhadap Konsentrasi Progesteron pada Tingkat Maturasi Oosit

Abstract

This research was conducted to determine the ffict of adding hormone FSH and PMSG in yitro ooq)te maturation medium on maturation rate and hormone progeiterone. In this experiment, ooq)tgs derivedfrom ovaries of slaughtered cattle at the slaughterhouse. Maturation medium using TCM-199 s-upplement"-! yryy I|ok, geitamicin 50ug/ml, FSH I0S/mTorPMSG 10 g/mlandthecultureofgranulosacells lxl05 cells/ml.In eachireatmentunitis"used'2| oocyt\quality AandB irltOO mtofimaturatioilnedium. RandomizedBlokDesigrl in factorial (2x2x4) was used inihis'expe:riment. The Factor A culture (without cells and granulosa cellil, B fabtorhormones (PMSG andFSH).Variables measuredwerethenumber maturation andprogesterone hormone-levels in maturation medium by using RH techniques. The results shown, there is no interaction of hormone addition in granulosa cell numbers on maturationlevel and progesterone concentration in maturation medium. GranulosaZell inmaturationmediumsignificantly (P<0.05) tnc-reasedthenumber ofooqttematurationinvitto. The highest maturation was obtained fiofi media with grdnulosa cells and FSH rif 7 I .25%, while the highest progesTerone obtainedfrom media with granulosa cells and PMSG I. 40 ng / ml.

Pengaruh urea dalam media maturasi in vitro terhadap tingkat maturasi oosit sapi

“…Maturasi oosit secara in vitro adalah sebuah proses yang dipengaruhi oleh individual oosit, yang disebabkan oleh adanya perbedaan dalam perkembangan dan pertumbuhan oosit yang dikoleksi dari ovarium dengan aktivitas intraovarian (Conti dan Franciosi, 2018). Faktor lain yang menyebabkan yakni kondisi kultur dan penambahan hormon dan media kultur (Mbemya et al, 2018), misalnya dengan penambahan serum buatan, foetal bovine serum (Widyastuti et al, 2015), maupun Follicle Stimulating Hormone (FSH) dan Pregnant Mare's Serum Gonatrotropin (PMSG) (Dianti et al, 2011). Media yang disuplementasi dengan serum perkembangan oosit lebih banyak dibandingkan media tanpa serum.…”

Relationship of Gonadotropin-Releasing Hormones (GnRH) Induction to Reproductive Performance in Batur Sheep

“…The higher number of follicles, the higher estrogen hormone produces. High estrogen hormone causes the secretion of LH which will break deGraff follicles in the ovary, so that ovulation occurs and sheep will show signs of estrus (Dianti et al, 2011). Furthermore, it was also reported that the modulation of the FSH-LH hormone induced by GnRH was able to increase the number of ovulating follicle waves (Sumaryadi et al, 2000;Putra and Kusumawati, 2014).…”