Pengaruh Konsentrasi ZPT Auksin Terhadap Pertumbuhan Tanaman Anggrek

Thamrin, Nining Triani

doi:10.55678/plantklopedia.v1i2.503

Cited by 2 publications

(3 citation statements)

References 4 publications

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“…Table 2 also shows the lowest yield of 0.89 units with a deviation value that is also not much different, so that this combination of treatments has an unfavorable effect on the growth of Dendrobium orchid plantlets. According to research by Lestari et al (2013) BAP is classified as a synthetic cytokinin whose use is influenced by other nutrients, so the addition of BAP can also affect growth hormone in orchids.…”

Section: Number Of Rootsmentioning

confidence: 99%

The Effect of Growmore and BAP (Benzyl Amino Purine) Concentrations on The Growth of Dendrobium bigiante agrihorti Orchid In-Vitro

Zanirah,

Sutini,

Pribadi

2023

JTEP-L

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The increase in production of Dendrobium bigiante agrihorti orchids by in vitro culture is to produce numerous and uniform seedlings. This study aimed to determine the effect of Growmore and BAP concentrations on the growth of orchid plantlets. The experiment consisted of two factors namely Growmore concentration (0; 3; 6; 9 ppm), and BAP concentrations (0; 1; 3; 5 ppm). The factors combination on ½ MS media was repeated 3 times and obtained 48 experimental units. Observation parameters included the percentage of live plantlets, number of roots, number of leaves, plant height (cm), and wet weight of plantlets (g). Results showed that a Growmore concentration of 3 ppm had a significant effect on the number of roots and plant height at 4.36 units and 4.17 cm. BAP concentration of 3 ppm also had a significant effect on the number of roots, plant height, and wet weight of respectively 4.72 units, 3.22 cm, and 0.63 g. A combination of Growmore 3 ppm + BAP 0 ppm produced the largest percentage of live planets at 100% and plant height at 7.37 cm, Growmore 9 ppm + BAP 0 ppm produced the largest number of roots at 6.33 units and Growmore 6 ppm + BAP 3 ppm produced the largest number of leaves at 8.78 strands. Keywords: BAP, Dendrobium bigiante, Growmore.

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Section: Number Of Rootsmentioning

confidence: 99%

The Effect of Growmore and BAP (Benzyl Amino Purine) Concentrations on The Growth of Dendrobium bigiante agrihorti Orchid In-Vitro

Zanirah,

Sutini,

Pribadi

2023

JTEP-L

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“…Penambahan BAP pada media kultur in vitro dapat memicu pembelahan sel dan morfogenesis. Penelitian yang dilakukan oleh Lestari et al (2013) menunjukkan bahwa pertumbuhan biji cenderung meningkat pada konsentrasi BAP lebih tinggi, yaitu pemberian BAP dengan konsentrasi 1,0-2,0 ppm. Berdasarkan uraian di atas, dilakukan penelitian yang mengkaji secara jelas mengenai komposisi nutrisi yang tepat pada tahap perkecambahan biji anggrek Grammatophyllum stapeliiflorum secara in vitro.…”

Section: Pendahuluanunclassified

“…Pemberian sitokinin pada masa perkecambahan dapat menstimulasi pembelahan sel dan mendorong perkecambahan biji. Berdasarkan penelitian Lestari et al (2013) mengenai perkecambahan biji Dendrobium laxiflorum J.J Smith secara in vitro dengan pemberian 1 mg/L BAP mampu mendukung perkecambahan biji anggrek hingga membentuk protokorm.…”

Section: Pembahasanunclassified

Perkecambahan Biji Anggrek Grammatophyllum stapeliiflorum Pada Media MS dengan Penambahan BAP Secara In Vitro

Hany,

Noli,

Suwirmen

2023

Al-Kauniyah J. Biol.

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AbstrakGrammatophyllum stapeliiflorum merupakan jenis anggrek epifit dengan pertumbuhan vegetatif dan generatif yang relatif lambat. Anggrek ini termasuk ke dalam kelompok CITES Apendiks II. Kultur in vitro merupakan usaha perbanyakan paling efektif untuk tanaman anggrek. Penggunaan media kultur dan Zat Pengatur Tumbuh (ZPT) yang tepat akan meningkatkan keberhasilan perkecambahan biji anggrek secara in vitro. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi media MS dan penambahan BAP terbaik terhadap perkecambahan anggrek G. stapeliiflorum secara in vitro. Penelitian ini menggunakan rancangan acak lengkap dengan 6 perlakuan dan 4 ulangan. Perlakuan berupa variasi konsentrasi media MS dan BAP, yaitu: MS penuh; MS ½ hara makro; MS ¼ hara makro; MS penuh + 1 ppm BAP; MS ½ hara makro + 1 ppm BAP; dan MS ¼ hara makro + 1 ppm BAP. Parameter yang diamati pada penelitian ini, yaitu waktu muncul protokorm dan persentase tahap perkecambahan biji. Data dianalisis menggunakan uji ANOVA dan uji lanjut Duncan New Multiple Range Test dengan taraf 5%. Hasil penelitian menunjukkan bahwa pemberian BAP mampu mempercepat waktu muncul protokorm. Konsentrasi media MS ¼ hara makro + 1 ppm BAP merupakan konsentrasi terbaik untuk perkecambahan biji anggrek tahap 0 hingga tahap 3, sedangkan konsentrasi media MS ¼ hara makro merupakan konsentrasi terbaik untuk mencapai tahap 4 perkecambahan biji anggrek G. stapeliiflorum secara in vitro.AbstractGrammatophyllum stapeliiflorum is a type of epiphytic orchid with relatively slow vegetative and generative growth. This orchid is included in the CITES Appendix II group. In vitro culture is the most effective propagation method for orchid plants. The use of appropriate culture media and growth regulators will increase the success of orchid seed germination in vitro. This study aims to determine the effect of the best concentration of MS media and the addition of BAP on the germination of G. stapeliiflorum orchids in vitro. This study used a completely randomized design with 6 treatments and 4 replications. The treatments consisted of varying concentrations of MS and BAP media, namely: full MS; MS ½ macro nutrients; MS ¼ macro nutrients; full MS + 1 ppm BAP; MS ½ macro nutrients + 1 ppm BAP; and MS ¼ macro nutrients + 1 ppm BAP. The parameters observed in this study were the time when the protocorm appeared and the percentage of seed germination stages. Data were analyzed using the ANOVA test and the Duncan New Multiple Range Test with a level of 5%. The results of the study showed that administration of BAP was able to speed up the time when protocorm appeared. MS media concentration ¼ macro nutrients + 1 ppm BAP is the best concentration for stage 0 to stage 3 orchid seed germination, while MS media concentration ¼ macro nutrients is the best concentration for achieving stage 4 germination of G. stapeliiflorum orchid seeds in vitro.

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Pengaruh Konsentrasi ZPT Auksin Terhadap Pertumbuhan Tanaman Anggrek

Cited by 2 publications

References 4 publications

The Effect of Growmore and BAP (Benzyl Amino Purine) Concentrations on The Growth of Dendrobium bigiante agrihorti Orchid In-Vitro

The Effect of Growmore and BAP (Benzyl Amino Purine) Concentrations on The Growth of Dendrobium bigiante agrihorti Orchid In-Vitro

Perkecambahan Biji Anggrek Grammatophyllum stapeliiflorum Pada Media MS dengan Penambahan BAP Secara In Vitro

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