Pengaruh Glukosa Tinggi terhadap Proliferasi, Migrasi dan Ekspresi Gen OCT-4 pada Kultur Sel Dermal Fibroblast Manusia

Abstract

Human dermal fibroblasts (HDF) termasuk sel mesenchymal yang diisolasi dari lapisan dermis kulit. HDF berpotensi digunakan untuk pengobatan penyembuhan luka berdasarkan pengobatan regeneratif. Peningkatan konsentrasi glukosa dapat merusak fungsi sel dan menghambat terapi penyembuhan luka. Penelitian ini dilakukan untuk mengkaji pengaruh glukosa tinggi pada proliferasi, migrasi dan ekspresi gen OCT-4 sel HDF sebagai model penyembuhan luka diabetes in vitro. Pada penelitian eksperimental ini, fibroblast diisolasi dari kulit setelah sirkumsisi, kemudian ditumbuhkan dalam medium Minimal Essential Medium (DMEM) Dulbecco lengkap dengan serum 10%. Untuk menguji efek glukosa tinggi pada proliferasi sel HDF dilakukan dengan uji CCK-8. Migrasi sel HDF dievaluasi menggunakan uji scratch-assay. RT-PCR digunakan untuk menentukan ekspresi gen OCT-4. Hasilnya menunjukkan bahwa glukosa tinggi (25 mM-50 mM) meningkatkan kemampuan proliferasi dan migrasi sel HDF. Efek glukosa tinggi tergantung pada dosis. Perlakuan glukosa pada dosis 75 mM akan menghambat kemampuan pertumbuhan HDF. Ekspresi gen OCT-4 meningkat secara bermakna pada pemberian glukosa dosis 25-50 mM. Hasil penelitian ini memberikan dasar untuk pengembangan terapi dalam kondisi diabetes bahwa terapi sel HDF dapat meningkatkan penyembuhan luka meskipun dalam kondisi glukosa tinggi.

The Subchronic Effect Of Water Extract Leaves Ofsoursop (Annona Muricata L.) Bax Expression Of Neuron Rat Hippocampus Gyrus Dentatus (Rattus Norvegicus)

“…Other processes of cell apoptosis may occur; 1) As a result of an intrinsic trigger from within the cell, 2) Triggered by an external "death activator" attached to receptors on the cell surface, and 3) Triggered by oxidizing agents from outside the cell (Hadi, 2011).…”

“…Another mechanism acetogenin can stop the cancer cell cycle in the G1 phase and inhibit the progress of the cell cycle towards the S phase by inducing the expression of p53, p21, Bax, and Bad in cancer cell lines (Hadi, 2011). Based on the description above acetogenin compounds can cause damage to some healthy cells through the mechanism of apoptosis which is thought to involve Bax, so it is necessary to research the effect of subchronic administration of soursop leaf water extract (Annona muricata) on Bax expression in neurons of the dentate gyrus hippocampus of rats (Rattus norvegicus) (Asyiyah, 2005).…”

Effects of Green Tea Leaf Extract on Viability, Apoptosis, and Expression of Interleukin-6 in Keloid Fibroblasts

“…Keloid fibroblast (KF) cell cultures were grown in 96 and 24 well culture dishes that were given growth media, namely Gibco Dulbecco's Modified Eagle Medium (DMEM), which has been mixed with Fetal Bovine Serum (FBS) 10% and Antibiotic-Antimicotic (AA) 1% and then incubated in a 5% Co2 incubator, 37°C with a cell count of 10,000 cells/cm 2 . 17 Each was divided into six treatment groups with four replicates, namely: DMEM-serum control (KS) as negative control, dexamethasone 100 µM (Dexa 100) as positive control, and four groups of green tea leaf extract 100 µg/mL (GTE 100), 200 µg/mL (GTE 200), 400 µg/mL (GTE 400) and 800 µg/mL (GTE 800).…”