Pengaruh Ekstrak Etanol 90% terhadap Kadar Malondialdehid (MDA) dan Superoksida Dismutase (SOD) Mencit Tikus yang Dipapar Asap Rokok

Prayitno, Sutrisno Adi; Kusnadi, Joni; Muritni, Erni Sofia

doi:10.18860/al.v6i1.5018

Cited by 2 publications

(2 citation statements)

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“…12 Pengaruh dari radikal bebas tersebut dapat ditekan melaui pemberian antioksidan. 13 Salah satu antioksidan yang sedang berkembang serta banyak beredar dan diminati oleh masyarakat adalah VCO. 14 Di Indonesia sendiri penggunaan VCO di tengah masyarakat dipercaya sebagai antivirus, antibakteri, antiprotozo, meningkatkan imunitas terhadap penyakit-penyakit dan kerap dipercaya dapat mencegah terjadinya obesitas.…”

Section: Pendahuluanunclassified

Pengaruh Pemberian Virgin Coconut Oil (VCO) Terhadap Kadar Hemoglobin dan Jumlah Eritrosit Tikus Putih Jantan Galur Wistar Yang Dipapar Asap Rokok

Simorangkir

2022

Scientificj

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Abstrak Pendahuluan: Virgin Coconut Oil (VCO) merupakan minyak kelapa murni yang berasal dari olahan daging kelapa segar. VCO memiliki kandungan antioksidan yang tinggi yang dapat berperan sebagai radical scavengers atau peredam reactive oxygen spesies (ROS). ROS terbesar adalah asap rokok yang dapat menyebabkan stress oksidatif bagi eritrosit. Tujuan penelitian: untuk mengetahui bagaimana pemberian VCO berpengaruh sebagai antioksidan terhadap kadar hemoglobin dan jumlah eritrosit tikus putih jantan galur wistar yang dipapar asap rokok. Metode: Desain penelitian ini adalah posttest only control group design. Sampel dalam penelitian sebanyak 24 ekor tikus jantan galur wistar usia 2-3 bulan yang dibagi menjadi 4 kelompok yang diberi perlakuan berbeda selama 14 hari yaitu kelompok kontrol negatif diberi pakan standart, kelompok kontrol positif diberi pakan standart dan dipapar asap rokok, kelompok perlakuan 1 (P1) dipapar asap rokok dan diberi VCO sebanyak 0,45 ml, kelompok P2 dipapar asap rokok dan diberi VCO sebanyak 0,9 ml. Pada hari ke-15 tikus dibedah untuk diperiksa darahnya. Kemudian data dianalisis dengan uji Anova pada tingkat kepercayaan 95%. Hasil: Secara statistik tidak terdapat perbedaan yang signifikan rerata kadar hemoglobin dan jumlah eritrosit tikus putih jantan galur wistar. Namun berdasarkan hasil pemeriksaan darah, kadar hemoglobin dan jumlah eritrosit kelompok control positif lebih rendah dibandingkan 3 kelompok lainnya. Serta kadar hemoglobin dan jumlah eritrosit yang diberi VCO dengan dosis maksimal 0,9 ml lebih tinggi dibandingkan dengan menggunakan dosis 0,45 ml. Kesimpulan: Pemberian VCO tidak dapat mencegah penurunan kadar hemoglobin dan jumlah eritrosit tikus putih jantan galur wistar yang dipapar asap rokok. Abstract Introduction: Virgin Coconut Oil(VCO) is virgin coconut oil which is derived from processed fresh coconut meat. VCO has a high antioxidant content where these antioxidants act as radical scavengers or reducer of reactive oxygen species (ROS). The biggest ROS is cigarette smoke which can causing oxidative stress for erythrocytes. Aims : to determine how the administration of VCO has an effect as an antioxidant on hemoglobin levels and the number of erythrocytes of male white rats of the Wistar strain exposed to cigarette smoke.Method: The design of this study was a posttest only control group design. The sample in this study were 24 male wistar rats aged 2-3 months. This study was divided into 4 groups that were given different treatments for 14 days, namely the negative control group was given standard feed, the positive control group was given standard feed and was exposed to cigarette smoke, treatment group 1 (P1) was exposed to cigarette smoke and given virgin coconut oil (VCO). 0.45 ml, group P2 was exposed to cigarette smoke and given 0.9 ml of VCO. On the 15th day the rats were dissected to check their blood. Then the data were analyzed by Anova test at 95% confidence level. Results: Statistically, there was no significant difference in the mean hemoglobin level and erythrocyte count of Wistar male white rats. However, based on the results of blood tests, the hemoglobin and erythrocyte counts in the positive group were lower than the other 3 groups. As well as hemoglobin levels and the number of erythrocytes given VCO with a maximum dose of 0.9 ml higher than using a dose of 0.45 ml. Conclusion: Giving VCO could not prevent a decrease in hemoglobin levels and the number of erythrocytes of male Wistar strain rats exposed to cigarette smoke.

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Section: Pendahuluanunclassified

Pengaruh Pemberian Virgin Coconut Oil (VCO) Terhadap Kadar Hemoglobin dan Jumlah Eritrosit Tikus Putih Jantan Galur Wistar Yang Dipapar Asap Rokok

Simorangkir

2022

Scientificj

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“…Luteinizing Hormone (LH) produced by anterior hypophysis serves to stimulate Leydig cells in testis to produce testosterone. Meanwhile, FSH, which is also produced by anterior hypophysis, influences Sertoli cells to secrete growth factor and form androgen binding protein (ABP) serving to bind intratesticular testosterone produced by leydig cells and triggering spermatogenesis (Sutikno, 2014). Therefore, decreased LH and FSH levels resulting from exposure to MSG may cause reduced sperm quality with mediation of reduced spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)

Rahmawati

Nasihun

Isradji

2019

Sains Med. J. Kedokt. dan Kesehat.

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Introduction: ROS is the most common cause of male infertility. Monosodium glutamate (MSG) is a compound which produces ROS and decreases FSH and LH levels. Continuous Multistage Countercurrent Extraction (CMCE) propolis extract as an antioxidant is evidently capable of reducing ROS concentration. Objective: this research aims at evaluating the effect of CMCE propolis extract administration on Leydig cells and Sertoli cells counts and sperm quality of MSG-induced male wistar rats. Methods: employing the Post Test Only Control Group Design, 18 rats were divided into 3 groups: control group, treated only with MSG (MS-G), and treatment groups, respectively treated with CMCE propolis extract at doses 8.3mg/day (CM8-G) and 10.8mg/day (CM10-G). CMCE propolis extract was given orally for 21 days. At the end of treatment, sperm and testis were taken to evaluate the Leydig cells and Sertoli cells counts and sperm quality. The sperm obtained from epididymis was analyzed using the WHO standards, while Leydig cells and Sertoli cells were counted from preparation using the HE stain method. Results: the Post Hoc analysis result shows that the Leydig and Sertoli cells counts and sperm quality of CM8-G and CM10-G are significantly higher than those of MS-G, p<0.05. The Leydig cells and Sertoli cells counts and sperm quality of CM10-G are significantly higher than those of CM8-G, p<0.05. Conclusion: CMCE propolis extract administration evidently increases the Leydig cells and Sertoli cells counts and sperm quality from the perspective of the count, motility and morphology of spermatozoa of MSG-induced male wistar rats.

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Pengaruh Ekstrak Etanol 90% terhadap Kadar Malondialdehid (MDA) dan Superoksida Dismutase (SOD) Mencit Tikus yang Dipapar Asap Rokok

Cited by 2 publications

References 13 publications

Pengaruh Pemberian Virgin Coconut Oil (VCO) Terhadap Kadar Hemoglobin dan Jumlah Eritrosit Tikus Putih Jantan Galur Wistar Yang Dipapar Asap Rokok

Pengaruh Pemberian Virgin Coconut Oil (VCO) Terhadap Kadar Hemoglobin dan Jumlah Eritrosit Tikus Putih Jantan Galur Wistar Yang Dipapar Asap Rokok

The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)

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