Pengaruh berbagai formulasi pengencer dasar air kelapa terhadap kualitas semen cair sapi PO (Peranakan Ongole) selama simpan dingin

Muhammad, Dedi; Isnaini, Nurul; Kuswati, Kuswati; Yekti, Aulia Puspita Anugra; Aryogi, Aryogi; Lutfi, Muhammad; Lukman, Haris; Susilawati, Trinil

doi:10.21776//ub.jiip.2019.029.01.01

Cited by 1 publication

(2 citation statements)

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“…After this, it is heated at a temperature of 50°C for approximately 20 minutes to deactivate the enzymes present in the coconut water. Additionally, 10 and 20 mg/ml of fructose, 270 ISSN 2721-1304 (Print), ISSN 2721-1207 (online) Copyright © 2023, Journal La Lifesci, Under the license CC BY-SA 4.0 1 mg/ml of penicillin antibiotic, sorbitol in g/L, 1 mg/ml of streptomycin sulfate, and 0.05 g/ml of gentamicin are added (Muhammad et al, 2019). Technical term abbreviations will be explained upon first use.…”

Section: Preparation Of Red Fruit Extract + Coconut Water Combination...mentioning

confidence: 99%

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Integrity of Sperm Cell Bali Bull Preserved at 5°C Using a Diluent Formulation of Extracted Red Fruit and Coconut Water

Ervandi

2023

JLL

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This study aims to determine the role of the best diluent in protecting the integrity of the spermatozoa membrane after storage using an alternative diluent formulation of red fruit extract (Pandanus Conoideus Lamk) and coconut water. An alternative diluent formulation featuring red fruit extract (Pandanus Conoideus Lamk) and coconut water was utilized. Fresh semen from Bali Bull was evaluated in the Integrated Field Laboratory, Department of Animal Science of Universitas Muhammadiyah Gorontalo. Bali bull sperm of high quality was diluted using a combination diluent containing red fruit extract (Pandanus Conoideus Lamk), green coconut water, and CEP-2 mixed with 10% egg yolk. Technical term abbreviations were explained at their initial use. Spermatozoa membrane integrity was assessed using the hypoosmotic swelling test (HOST), while chlortetracycline (CTC) fluorescent dye was used to evaluate spermatozoa capacitation and acrosome reaction. The randomized group design (RAK) was used in the research, consisting of four conditions and 10 replications. P0 served as the control group, which received CEP-2 plus 10% egg yolk. P1 was administered EBM diluent AK enriched with 15% KT, while P2 received EBM diluent AK plus 15% KT and fructose 1 mg/ml. P3 was given EBM diluent AK plus 15% KT and fructose 2 mg/ml. Analysis of variance was used to examine the resulting data. The findings indicated that CEP-2 plus 10% egg yolk diluent was more effective than the diluent formulations containing red fruit extract and coconut water. Spermatozoa with intact membranes and no capacitation remain high, while those with capacitation and acrosome reaction remain low.

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Section: Preparation Of Red Fruit Extract + Coconut Water Combination...mentioning

confidence: 99%

“…These can potentially harm spermatozoa due to exposure to chemical residues (Salim, et al, 2019). The inclusion of coconut water in the diluent can provide adequate simple carbohydrates, minerals and other substances necessary for the survival of spermatozoa (Muhammad et al, 2019;Farapti and Sayogo, 2018).…”

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confidence: 99%

Integrity of Sperm Cell Bali Bull Preserved at 5°C Using a Diluent Formulation of Extracted Red Fruit and Coconut Water

Ervandi

2023

JLL

View full text Add to dashboard Cite

show abstract

Pengaruh berbagai formulasi pengencer dasar air kelapa terhadap kualitas semen cair sapi PO (Peranakan Ongole) selama simpan dingin

Cited by 1 publication

References 3 publications

Integrity of Sperm Cell Bali Bull Preserved at 5°C Using a Diluent Formulation of Extracted Red Fruit and Coconut Water

Integrity of Sperm Cell Bali Bull Preserved at 5°C Using a Diluent Formulation of Extracted Red Fruit and Coconut Water

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