Pengaruh BAP Dan NAA Terhadap Waktu Pertumbuhan Tanaman Kantong Semar(Nepenthes adrianii) Secara In-Vitro

Nuryadin, Egi

doi:10.23969/biosfer.v3i1.1199

Cited by 2 publications

(2 citation statements)

References 1 publication

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“…Shoots appear when the ratio between cytokinin hormone is higher than auxin hormone so that the cell does cell division continuously and differentiates towards the formation of shoots Enni Suwarsi Rahayu & Nabila Cyntia Banowati / Biosaintifika 14 (3) (2022): 417-421 (Urry et al, 2017). Nuryadin (2018) reported that the time of shoot initiation was not influenced by the interaction of the BAP hormone with NAA but from the BAP hormone independently. The number of shoots was influenced by BAP concentration, sucrose concentration, and their interactions.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Multiplication of Nepenthes mirabilis Lour (Druce) with Variations Concentration of Sucrose and BAP

Banowati

Rahayu

2022

J Bio Bio Edu

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Nepenthes mirabilis (Lour) Druce is a rare ornamental plant and has high economic value so it needs to be preserved. This study aimed to analyze effect of sucrose and BAP concentrations and their optimal interactions in the in vitro multiplication of Nepenthes mirabilis. Explants in the form of in vitro plantlets from Laboratory of Plant Tissue Culture, Department of Biology, Semarang State University. The treatment was carried out by adding sucrose and BAP to murashige skoog (MS) media with various concentrations. The treatments were arranged in two factor completely randomized design with four replications. Plantlets were planted in the treatment medium for 10 weeks, then every week the growth of shoots and leaves of N. mirabilis was observed. The data were analyzed by two-way analysis of variance and Duncan's test. The results showed that concentration of sucrose, BAP, and their interactions affected the growth of explants. Plantlets grown on media with sucrose concentration 20 g/l produced the highest number of shoots, media with concentrations BAP 3 and 2 ppm produced the highest number of shoots and number of leaves. Medium ½ MS with varying concentrations of sucrose 20 g/l with 3 ppm BAP and sucrose 20 g/l with 2 ppm BAP resulted in the highest growth in the number of shoots and number of leaves. The composition of this medium can be used for the multiplication of N. mirabilis.

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Section: Resultsmentioning

confidence: 99%

In Vitro Multiplication of Nepenthes mirabilis Lour (Druce) with Variations Concentration of Sucrose and BAP

Banowati

Rahayu

2022

J Bio Bio Edu

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“…Nepenthes can be propagated conventionally using seeds and stem cuttings or nonconventional propagation using tissue culture techniques (Devi et al, 2013;Dinarti et al, 2010;Rosmaina and Aryani, 2015;Sukamto, 2011;Nuryadin, 2018). However, Nepenthes is a dioecious plant whose insect such as Diptera and Hymenoptera assists the pollination, or sometimes aided by the wind, and many pitchers have offspring from crosses of several species and the resulting impurity seed (Cheek and Jebb, 2001).…”

Section: Nepenthes Reinwardtiana Miq) Through Callus Inductionmentioning

confidence: 99%

PROPAGATION OF PITCHER PLANTS (Nepenthes gracilis KORTH. AND Nepenthes reinwardtiana MIQ.) THROUGH CALLUS INDUCTION

Novitasari

Isnaini²

2021

Agric

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In vitro propagation of pitcher plants is still limited only using seeds, while the other in vitro methods using leaf explants such as embryogenesis, organogenesis, and callus culture has not been widely reported. The research aims to study the growth response of leaf explants in two species of pitcher plants (Nepenthes gracilis and Nepenthes reinwardtiana), which formed callus in several treatment media combinations. Leaf pieces were taken from a 4-month pitcher plant culture were grown on the treatment media, namely modified media of Murashige & Skoog with a half concentration (½ MS) added 2.4-Dichlorophenoxoxyacetate (2.4-D) with a concentration of 0, 0.5, 1, 1.5, and 2 mg L-1 and kinetin (0.5 mg L-1). The treatment was then stored in a dark environment to induce callus formation. The observation for 12 weeks showed that the time of callus formation in two species of pitcher plant observed was not significantly different. Both species of pitcher plants begin to form callus in the fourth week after treatment. None of the leaf explants were planted on the control medium without hormones formed callus. The best medium for callus induction in N. gracilis is ½ MS medium added with 2 mg L-1 2,4-D and 0.5 mg L-1 kinetin, with callus morphology brownish-white with friable texture. In comparison, the optimum callus media from leaf explant of N. reinwardtiana has not been obtained yet. Thus further research is still needed.

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Pengaruh BAP Dan NAA Terhadap Waktu Pertumbuhan Tanaman Kantong Semar(Nepenthes adrianii) Secara In-Vitro

Cited by 2 publications

References 1 publication

In Vitro Multiplication of Nepenthes mirabilis Lour (Druce) with Variations Concentration of Sucrose and BAP

In Vitro Multiplication of Nepenthes mirabilis Lour (Druce) with Variations Concentration of Sucrose and BAP

PROPAGATION OF PITCHER PLANTS (Nepenthes gracilis KORTH. AND Nepenthes reinwardtiana MIQ.) THROUGH CALLUS INDUCTION

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