Penetration of Fluoride into Natural Plaque Biofilms

Abstract

Caries occurs at inaccessible stagnation sites where plaque removal is difficult. Here, the penetration through plaque of protective components, such as fluoride, is likely to be crucial in caries inhibition. We hypothesized that topically applied fluoride would readily penetrate such plaque deposits. In this study, plaque biofilms generated in vivo on natural enamel surfaces were exposed to NaF (1000 ppm F-) for 30 or 120 sec (equivalent to toothbrushing) or for 30 min. Biofilms were then sectioned throughout their depth, and the fluoride content of each section was determined with the use of a fluoride electrode. Exposure to NaF for 30 or 120 sec increased plaque fluoride concentrations near the saliva interface, while concentrations near the enamel surface remained low. Fluoride penetration increased with duration of NaF exposure. Removal of exogenous fluoride resulted in fluoride loss and redistribution. Penetration of fluoride into plaque biofilms during brief topical exposure is restricted, which may limit anti-caries efficacy.

Adhesion of Streptococcus sanguinis to Dental Implant and Restorative Materials in vitro

“…Thus, the fluoride released might have soundly contributed to the low vitality and adhesion of S. sanguinis cells. By way of practical application, the extent to which topically applied fluoride penetrates plaque biofilm is an important subject, since even limited fluoride penetration may serve to inhibit growth of plaque bacteria 26) . Therefore, a constant release of fluoride ions from a substratum like composite, in addition to topically applied fluoride, might result in an improved inhibition of plaque bacteria.…”

Wear and Corrosion Interactions on Titanium in Oral Environment: Literature Review

“…The exposure of structural materials to oral fluids, including acidic substances produced by bacterial metabolism, is associated to the corrosion of the implant fixture-abutment joint [16]. In addition, fluorides can be accumulated in biofilms depending on their structure and composition, physico-chemical properties of the solute, and biofilm thickness [96][97][98][99]. Due to the diffusion of F -ions through extracellular matrix, fluorides can also reach oral tissues and other micro-areas in the biofilm [96,97].…”

“…In addition, fluorides can be accumulated in biofilms depending on their structure and composition, physico-chemical properties of the solute, and biofilm thickness [96][97][98][99]. Due to the diffusion of F -ions through extracellular matrix, fluorides can also reach oral tissues and other micro-areas in the biofilm [96,97]. The lowering of pH caused by the release of lactic acid from microbial metabolism in the biofilm can be responsible for a considerable concentration of HF that can corrode titanium and feldspar-based porcelain surfaces of dental implant-supported prostheses.…”

Adherence of Streptococcus sanguinis and Streptococcus mutans to salivacoated S-PRG resin blocks

“…Also, the release of fluoride from GICs can prevent caries progression by interfering with the growth or metabolism of the remaining cariogenic bacteria 19,20) . Fluoride penetration into dental plaque is an important subject, since even limited fluoride penetration may serve to inhibit the growth of plaque bacteria 21) . Fluoride is known to inhibit the biosynthetic metabolism of bacteria, but these antimicrobial effects in caries prevention are often regarded as minor compared with the direct interactions of fluoride with the hard tissue during caries development.…”