Penetration kinetics of four mouthrinses into Streptococcus mutans biofilms analyzed by direct time-lapse visualization

Wakamatsu, Rika; Takenaka, Shoji; Ohsumi, Tatsuya; Terao, Yutaka; Ohshima, Hayato; Okiji, Takashi

doi:10.1007/s00784-013-1002-7

Cited by 26 publications

(31 citation statements)

References 57 publications

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“…However, the conventional tests of antibiotic susceptibilities, such as the Kirby-Bauer (disc diffusion) test or broth dilution measurements exclude the conditions of biofilm formation and thus do not reflect the effectiveness of antimicrobials in vivo. Moreover, fluorescence-induced confocal laser scanning microscopy (CLSM) has been shown to be a powerful technique that reveals a three-dimensional structural image with microscopic resolution and allows for quantitative assessment of the bioðlm constituents [4][5][6][7]. However, since the extracellular polymeric substance (EPS) is a complex mixture of different biopolymers with a large number of potential binding sites [8], it is difficult to design a suitable protocol to stain the entire EPS, which limits the application of CLSM in EPS identification and quantification.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antibiotic effects on Pseudomonas aeruginosa biofilm using Raman spectroscopy and multivariate analysis

Jung

Nam

Choi

et al. 2014

Biomed. Opt. Express

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Abstract:We investigate the mode of action and classification of antibiotic agents (ceftazidime, patulin, and epigallocatechin gallate; EGCG) on Pseudomonas aeruginosa (P. aeruginosa) biofilm using Raman spectroscopy with multivariate analysis, including support vector machine (SVM) and principal component analysis (PCA). This method allows for quantitative, label-free, non-invasive and rapid monitoring of biochemical changes in complex biofilm matrices with high sensitivity and specificity. In this study, the biofilms were grown and treated with various agents in the microfluidic device, and then transferred onto gold-coated substrates for Raman measurement. Here, we show changes in biochemical properties, and this technology can be used to distinguish between changes induced in P. aeruginosa biofilms using three antibiotic agents. The Raman band intensities associated with DNA and proteins were decreased, compared to control biofilms, when the biofilms were treated with antibiotics. Unlike with exposure to ceftazidime and patulin, the Raman spectrum of biofilms exposed to EGCG showed a shift in the spectral position of the CH deformation stretch band from 1313 cm −1 to 1333 cm −1 , and there was no difference in the band intensity at 1530 cm −1 (C = C stretching, carotenoids). The PCA-SVM analysis results show that antibiotic-treated biofilms can be detected with high sensitivity of 93.33%, a specificity of 100% and an accuracy of 98.33%. This method also discriminated the three antibiotic agents based on the cellular biochemical and structural changes induced by antibiotics with high sensitivity and specificity of 100%. This study suggests that Raman spectroscopy with PCA-SVM is potentially useful for the rapid identification and classification of clinically-relevant antibiotics of bacteria biofilm. Furthermore, this method could be a powerful approach for the development and screening of new antibiotics.

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Section: Introductionmentioning

confidence: 99%

Evaluation of antibiotic effects on Pseudomonas aeruginosa biofilm using Raman spectroscopy and multivariate analysis

Jung

Nam

Choi

et al. 2014

Biomed. Opt. Express

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“…Diluted solutions were centrifuged at 2,000 g for 10 minutes to remove any debris, and the supernatants were filter sterilized. The saliva solution was used to coat discs with salivary pellicles [18,23]. All study protocols were approved by the Ethics Committee for Clinical Research of Niigata University (approval no.…”

Section: Saliva Collection and Processingmentioning

confidence: 99%

Adjunct use of mouth rinses with a sonic toothbrush accelerates the detachment of a Streptococcus mutans biofilm: an in vitro study

Ohsumi

Takenaka²,

Sakaue

et al. 2020

Preprint

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Background: The aim of this in vitro study was to examine the possible enhancement of the biofilm peeling effect of a sonic toothbrush following the use of an antimicrobial mouth rinse. Methods:The biofilm at a noncontact site in the interdental area was treated by sound wave convection with the test solution or by immersion in the solution. The biofilm peeling effect was evaluated by determining the bacterial counts and performing morphological observations. A Streptococcus mutans biofilm was allowed to develop on composite resin discs by cultivation with stirring at 50 rpm for 72 hours. The specimens were then placed in recesses located between plastic teeth and divided into an immersion group and a combination group. The immersion group was treated with phosphate buffer, Peridex™ mouth rinse or Listerine ® mouth rinse. The combination group was treated with Peridex™ or Listerine ® and a sonic toothbrush.

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“…Wakamatsu et al have reported the penetration kinetics of mouthrinses into in vitro Streptococcus mutans biofilms by direct time-lapse microscopic analysis. The antimicrobial penetration was critically restricted within 30 s of exposure; the average penetration velocity was ranging from 4.2 to 30.1 µm/min [13]. This phenomenon can be explained by retarded penetration due to degradation and/or modification by the biofilm matrix.…”

Section: Introductionmentioning

confidence: 97%

Adverse Influences of Antimicrobial Strategy against Mature Oral Biofilm

Takenaka¹,

Oda²,

Domon³

et al. 2016

Microbial Biofilms - Importance and Applications

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Penetration kinetics of four mouthrinses into Streptococcus mutans biofilms analyzed by direct time-lapse visualization

Abstract: The mouthrinses tested showed different levels of biofilm penetration. The essential oil rinse was superior to other rinses by all three of the in vitro measurements performed.

Cited by 26 publications

References 57 publications

Evaluation of antibiotic effects on Pseudomonas aeruginosa biofilm using Raman spectroscopy and multivariate analysis

Evaluation of antibiotic effects on Pseudomonas aeruginosa biofilm using Raman spectroscopy and multivariate analysis

Adjunct use of mouth rinses with a sonic toothbrush accelerates the detachment of a Streptococcus mutans biofilm: an in vitro study

Adverse Influences of Antimicrobial Strategy against Mature Oral Biofilm

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