PENETRATION, DEVELOPMENT, AND REPRODUCTION OF Meloidogyne enterolobii ON Psidium SPECIES AND INDUCED CELLULAR RESPONSES IN THE ROOTS

Abstract

Meloidogyne enterolobii has severely compromised the main guava producing regions in Brazil, thereby stimulating the development of resistant varieties for efficient management of this nematode. This study aimed to assess the penetration, development, reproduction, and induced cellular responses of M. enterolobii in roots of four species of the genus Psidium (P. guajava‘Paluma’, P. guineense, P. cattleyanum, and P. friedrichstalianum) from the Active Germplasm Bank (BAG) of the Center for Agricultural Research of the Semi-arid Tropics (Embrapa Semi-arid) located in Petrolina, PE, Brazil. Three experiments were carried out to assess the penetration, development, and reproduction of the nematode and, subsequently, histological analyses were performed. Nematode penetrated indistinctly in both resistant and susceptible species, but only in ‘Paluma’ an evolution was observed from vermiform juveniles to “sausage” and globose forms in experiment 1. In experiment 2, all species presented “sausage” forms of nematode, but only in ‘Paluma’, these forms reached adult female stages. In experiment 3, only ‘Paluma’ was susceptible to M. enterolobii, with reproduction factor above unity. Histological analyses showed that, except for P. guajava, the other Psidium species presented poorly developed feeding sites at 20 days after inoculation.

Reaction of Psidium guineense and Psidium guajava genotypes to infection of Meloidogyne enterolobii

“…Sousa et al [15], indicates that the resistance to M. enterolobii, observed for this species is given mainly by a developmental delay and life cycle extension. Thus, the 60-day pathogen infection period used in this study may be insuf icient to accurately measure the response of these genotypes, taking into account only this criterion.…”

“…After the infestation period and root extraction each genotype was evaluate to measure their reaction to the parasite. The cultivar Paluma chosen as a control genotype, which was previously reported to be susceptible to M. enterolobii [12][13][14][15]22] exhibited the highest value of galls index (GI=2.57).…”

“…Psidium species, such as P. guineense and P. cattleyanum can be a source of resistance, which makes them important for the control of the damages caused by the nematode in question [12][13][14]. In the various studies aiming at the analysis of M. enterolobii infection on fruit plants, the period between inoculation and root extraction ranged from 60 to 135 days, with 120 days being the most common interval used for this stage [11][12][13]15], which makes the process of evaluation quite long and costly due to the crop handling costs of the plants. The objective of this work was to evaluate the response of genotypes of P. guinense and P. guajava to the plant parasitic nematode M. enterolobii, in an early manner, with an inoculation period of 60 days.…”

Perspectivas Para O Mercado Da Goiaba

“…al., 2004, MAIA, 2021. Em relação a outras espécies deste grupo, muitas também apresentam interesse econômico, principalmente na produção de madeira (SOUSA et. al., 2017).…”

“…2017). A goiaba atingiu uma grande importância econômica no mundo todo, principalmente nas regiões tropicais e subtropicais, e isso se deve à grande presença de derivados da fruta nos alimentos (SOUSA et. al., 2017).…”