Penetration and preferential binding of charged nanoparticles to mixed lipid monolayers: interplay of lipid packing and charge density

Chaudhury, Anurag; Debnath, Koushik; Bu, Wei; Jana, Nikhil R.; Basu, J. K.

doi:10.1039/d0sm01945c

Cited by 5 publications

(7 citation statements)

References 73 publications

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“…This can be ascribed to the lowest surface charge density of O1G1 among all the cases, evident from the highest mean molecular area value of its corresponding monolayer at 32 mN/m. The charge density of DPPG domains in two-component mixed floating lipid monolayers with similar compositions to those used in this work has recently been quantified by our group in a separate work, 67 which also reveals the lowest charge density of the DPPG phase in DOPC/DPPG-based membranes.…”

Section: ■ Results and Discussionmentioning

confidence: 60%

Compressibility of Multicomponent, Charged Model Biomembranes Tunes Permeation of Cationic Nanoparticles

et al. 2021

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Cells respond to external stress by altering their membrane lipid composition to maintain fluidity, integrity and net charge. However, in interactions with charged nanoparticles (NPs), altering membrane charge could adversely affect its ability to transport ions across the cell membrane. Hence, it is important to understand possible pathways by which cells could alter zwitterionic lipid composition to respond to NPs without compromising membrane integrity and charge. Here, we report in situ synchrotron X-ray reflectivity (XR) measurements to monitor the interaction of cationic NPs in the form of quantum dots, with phase-separated supported lipid bilayers of different compositions containing an anionic lipid and zwitterionic lipids having variable degrees of stiffness. We observe that the extent of NP penetration into the respective membranes, as estimated from XR data analysis, is inversely related to membrane compression moduli, which was tuned by altering the stiffness of the zwitterionic lipid component. For a particular membrane composition with a discernible height difference between ordered and disordered phases, we were able to observe subtle correlations between the extent of charge on the NPs and the specificity to bind to the charged and ordered phase, contrary to that observed earlier for phase-separated model biomembranes containing no charged lipids. Our results provide microscopic insight into the role of membrane rigidity and electrostatics in determining membrane permeation. This can lead to great potential benefits in rational designing of NPs for bioimaging and drug delivery applications as well as in assessing and alleviating cytotoxicity of NPs.

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Section: ■ Results and Discussionmentioning

confidence: 60%

Compressibility of Multicomponent, Charged Model Biomembranes Tunes Permeation of Cationic Nanoparticles

et al. 2021

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“…Although the presence of buffer with salt can considerably screen the electrostatic interactions and the high zeta potential of the CQDs, the estimated Debye screening length of ∼0.8 nm (ref. 57) for the buffer used and the high charge density of the lipid membranes 43 suggest that the effective interaction strength would still be significant. Providing an exact quantification of the interaction energy between the QDs and the membrane as well as between the respective QDs would be challenging 13 given the complex geometry of the QDs and is outside the scope of this work.…”

Section: Discussionmentioning

confidence: 99%

“…DPPC:DPPG-based 2-component membranes have a higher charge density than the membranes having a fluidic component in lieu of DPPC. 43 An earlier theoretical study of oppositely charged proteins with a mixed lipid membrane having charged/uncharged lipids revealed that the protein coverage as well as the free adsorption energy is higher when the adsorbate molecules bind onto a more densely charged membrane. 69 Thus, the higher charge density of P1G1 not only leads to a higher adsorbed concentration of QDs on P1G1 but also a stronger binding affinity.…”

Section: Discussionmentioning

confidence: 99%

“…13,33,34 While most of these studies involved the use of single-component lipid membranes, 25,26,[35][36][37][38][39] some reports by us 40 and others 20,34,41,42 considered multi-component systems to observe the interplay of the different parameters that contribute to the binding phenomenon. In our recent work, we observed that fluid-like two-component lipid membranes 43,44 were disrupted to a larger extent by cationic quantum dots (CQDs) as compared to more ordered membranes. It was also observed that these CQDs had a higher binding affinity to the membrane (with one of the lipid components as negatively charged) that had higher packing and, hence, higher charge density.…”

Section: Introductionmentioning

confidence: 99%

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Spontaneous unbinding transition of nanoparticles adsorbing onto biomembranes: interplay of electrostatics and crowding

Chaudhury,

Debnath,

Jana

et al. 2024

Nanoscale

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“…Therefore, any dysfunction on the LS mechanical properties or modification of its composition may be harmful for health [32][33][34][35][36][37][38]. However, to date there is an important lack of knowledge about the true impact of inhaled particles in the respiratory cycle, even though it is clear that the deposition of particles on LS alters the breathing mechanics, the use of particles, even inhaled ones, in therapy and diagnosis is undergoing continuous growth [39][40][41][42][43]. Therefore, it is necessary to deepen the current understanding of the interactions of different types of particulate pollutants with LS for predicting their potential toxicity [44][45][46].…”

Section: Introductionmentioning

confidence: 99%

Fluid Films as Models for Understanding the Impact of Inhaled Particles in Lung Surfactant Layers

Guzmán

2022

Coatings

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Pollution is currently a public health problem associated with different cardiovascular and respiratory diseases. These are commonly originated as a result of the pollutant transport to the alveolar cavity after their inhalation. Once pollutants enter the alveolar cavity, they are deposited on the lung surfactant (LS) film, altering their mechanical performance which increases the respiratory work and can induce a premature alveolar collapse. Furthermore, the interactions of pollutants with LS can induce the formation of an LS corona decorating the pollutant surface, favoring their penetration into the bloodstream and distribution along different organs. Therefore, it is necessary to understand the most fundamental aspects of the interaction of particulate pollutants with LS to mitigate their effects, and design therapeutic strategies. However, the use of animal models is often invasive, and requires a careful examination of different bioethics aspects. This makes it necessary to design in vitro models mimicking some physico-chemical aspects with relevance for LS performance, which can be done by exploiting the tools provided by the science and technology of interfaces to shed light on the most fundamental physico-chemical bases governing the interaction between LS and particulate matter. This review provides an updated perspective of the use of fluid films of LS models for shedding light on the potential impact of particulate matter in the performance of LS film. It should be noted that even though the used model systems cannot account for some physiological aspects, it is expected that the information contained in this review can contribute on the understanding of the potential toxicological effects of air pollution.

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Penetration and preferential binding of charged nanoparticles to mixed lipid monolayers: interplay of lipid packing and charge density

Abstract: Designing of nanoparticles (NPs) for biomedical applications or mitigating their cytotoxic effects requires microscopic understanding of their interactions with cell membranes.

Cited by 5 publications

References 73 publications

Compressibility of Multicomponent, Charged Model Biomembranes Tunes Permeation of Cationic Nanoparticles

Compressibility of Multicomponent, Charged Model Biomembranes Tunes Permeation of Cationic Nanoparticles

Spontaneous unbinding transition of nanoparticles adsorbing onto biomembranes: interplay of electrostatics and crowding

Fluid Films as Models for Understanding the Impact of Inhaled Particles in Lung Surfactant Layers

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