Penetration and maturation of Schistosoma mansoni in suckling and adult Swiss Webster and DBA/2 mice

Activity of liver microsomal enzymes during the chronic phase of murine schistosomiasis

“…The differences between the two strains could be explained at least in part by differences in the number of parasite eggs harbored in the liver parenchyma. As demonstrated by Fidalgo-Neto et al (11) in mice infected with 100 cercariae on postnatal day 10, although skin penetration by cercariae was very effective and did not differ between the SW and DBA/2 strains, the proportion of penetrating cercariae that further developed into adult worms -and thus the number of eggs trapped in the host's liver on PID90 -was higher in the former strain.…”

“…Statistical comparisons within each mouse strain were made by the Kruskal-Wallis test followed by the Mann-Whitney U-test, with the level of significance set at P < 0.05. SW and DBA/2 mice were infected with S. mansoni (BH strain) cercariae as described in detail elsewhere (11). Briefly, 10-day-old pups were transferred to Petri dishes (5 cm in diameter, one pup per dish) containing 3 mL dechlorinated water with 100 cercariae.…”

Modulation of expression and activity of cytochrome P450s and alteration of praziquantel kinetics during murine schistosomiasis

“…Parasites and infection -Mice were infected with a Belo Horizonte isolate of S. mansoni as described previously by Fidalgo-Neto et al (2004). Briefly, 10-day old pups were transferred to Petri dishes (5 cm in diameter, 1 pup per dish) containing 3 mL of dechlorinated water with 100 recently-shed cercariae.…”

“…Eggs were recovered and counted according to previously described methods (Cheever 1968, Fidalgo-Neto et al 2004. Briefly, livers were excised, placed into conical flasks and digested with a 5% potassium hydroxide solution at 37ºC for 16 h. Aliquots (50 µL) of the digests were then placed onto microscope slides underneath a cover slip and the eggs were counted with a 100x magnification.…”

“…In experimentally infected mice, S. mansoni matures over a five-week period and the egg laying period begins approximately 30 days after infection (Fidalgo-Neto et al 2004). If the infection continues after this time, granulomas are formed around the schistosome eggs harboured in the liver parenchyma.…”