Penetration ability of different irrigants into dentinal tubules

Berutti, Elio; Marini, R; Angeretti, A

doi:10.1016/s0099-2399(97)80342-1

Cited by 234 publications

(187 citation statements)

References 13 publications

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“…21,22 A previous study showed that some canal irrigants could penetrate up to 130 µm from the canal lumen and eliminate bacteria. 23 However, heavy bacterial infection inside dentinal tubules may be present at depths of 400 µm. 24 For this reason, the present study evaluated reduction in dentin microhardness at 100 µm and 500 µm from the canal lumen, similarly to the study of Saghiri et al 12 Table 1.…”

Section: Discussionmentioning

confidence: 99%

Effect of final irrigation protocols on microhardness reduction and erosion of root canal dentin

et al. 2017

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This study aimed to evaluate the effect of final irrigation protocols on microhardness reduction and erosion of root canal dentin. Sixty root canals from mandibular incisors were instrumented and randomly divided into six groups (n = 10) according to the irrigant used: QMiX, 17% EDTA, 10% citric acid (CA), 1% peracetic acid (PA), 2.5% NaOCl (solution control), and distilled water (negative control). The chelating solutions were used to irrigate the canal followed by 2.5% NaOCl as a final flush. After the irrigation protocols, all specimens were rinsed with 10 mL of distilled water to remove any residue of the chemical solutions. Before and after the final irrigation protocols, dentin microhardness was measured with a Knoop indenter. Three indentations were made at 100 µm and 500 µm from the root canal lumen. Afterwards, the specimens were prepared for scanning electron microscopic analysis and the amount of dentin erosion was examined. Wilcoxon and Kruskal-Wallis tests were used to analyze the results with a significance level set at 5%. At 100 µm, all protocols significantly reduced dentin microhardness (p < .05), while at 500 µm, this effect was detected only in the EDTA and QMiX groups (p < .05). CA was the irrigant that caused more extensive erosion in dentinal tubules, followed by PA and EDTA. QMiX opened dentinal tubules, but did not cause dentin erosion. Results suggest that QMiX and 17% EDTA reduced dentin microhardness at a greater depth. Additionally, QMiX did not cause dentin erosion.

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Section: Discussionmentioning

confidence: 99%

Effect of final irrigation protocols on microhardness reduction and erosion of root canal dentin

et al. 2017

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“…The bacteria with their by-products and necrotic debris in smear layers compromise the disinfection process (5) and decrease dentin permeability, hampering the diffusion of antimicrobial agents like irrigants into root dentin (6).…”

Section: Discussionmentioning

confidence: 99%

“…EDTA has been suggested and widely used as an irrigation solution because it chelates calcium ions and removes the mineralized portion of smear layers (5,6,8). Although EDTA has a long history as an endodontic chelating agent, it has limited or no antibacterial activity (10) and a high surface tension value (16).…”

Section: Discussionmentioning

confidence: 99%

“…The smear layer may delay or prevent penetration of antimicrobial agents, such as endodontic irrigants. In an ex vivo histological study on human teeth, it has been found that the bactericidal effectiveness of endodontic irrigants depends on their ability to penetrate the infected dentinal tubules and this process is undoubtedly influenced by the presence or absence of smear layer (6). Fogel and Pashley (7) have previously shown that formation of smear layer reduced root dentin permeability after treatment with 5% NaOCl, but it increased many times after treatment with citric acid.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Effect and Surface Tension of Some Chelating Solutions with Added Surfactants

2016

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This study assessed the antimicrobial efficacy and surface tension of established irrigating solutions with a new experimental chelating solution in infected dentin tubes. Twenty-five specimens were randomly assigned to each of the irrigating solutions. Twenty specimens were used as negative and positive controls. After 21 days of contamination with E. faecalis, the irrigating solutions MTAD, QMiX and Tetraclean NA were delivered into each infected root canal. The solutions were removed and dentin samples were withdrawn from the root canals with sterile low-speed round burs with increasing ISO diameters. The dentin powder samples obtained with each bur were immediately collected in separate test tubes containing 3 mL of BHI broth. After that, 100 μL from each test tube was cultured on blood agar. The grown colonies were counted and recorded as colony-forming units (CFU). The surface tension of the irrigants was measured using a Cahn DCA-322 Dynamic Contact Angle Analyzer. A Kruskal Wallis nonparametric ANOVA and a Friedman test were used (p<0.05). Tetraclean NA showed lower surface tension and CFU values than MTAD and QMiX. Better antibacterial action and low surface tension were observed for Tetraclean NA, probably due to the improved penetration into the root canal and dentinal tubes

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“…Although various fungi, viruses, archaea contribute to the microbial diversity in endodontic infections, but the most common micro organism occurring in these infections are bacteria's [1]. Some microorganisms are resistant to antimicrobial treatment, it includes various Gram negative anaerobic rods such as Fusobacterium nucleatum, Prevotella species and gram positive bacterias such as Streptococcus gordonii, Enterococcus faecalis, Actinomyces species, etc [2]. The Microbial infection produce by these organisms plays an important role in the development of necrosis in dental pulp and leading to the formation of periapical lesions [3].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Photodynamic Therapy (PDT) in Disinfection of Deeper Dentinal Tubules in a Root Canal System: An In Vitro Study

Bumb

Bhaskar

Agali

et al. 2014

JCDR

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Context: The success of endodontic treatment therapy depends on how well we eliminate pathogenic microflora from the root canal system as micro organism as the major cause of root canal infection. Conventional root canal treatment can fail if microorganisms cannot be removed sufficiently by thorough cleaning, shaping of root canal. Newer modalities such as photodynamic therapy are being tried now a days for disinfection of root canals. Aim & Objectives:The basic aim of this study was assessment of the antimicrobial efficacy of Photodynamic Therapy in deeper dentinal tubules for effective disinfection of root canals using microbiological and scanning electron microscopic examination in vitro. Materials and Methods:The study was conducted at Teerthanker Mahaveer Dental College & Research Centre. The teeth required for study was collected from Department of Oral and Maxillofacial Surgery. Only freshly extracted 20 intact, non carious single rooted teeth which were indicated for orthodontic treatment were taken for this study. Statistical analysis was done using Student's Unpaired t-test were at (p<0.001) was found to be highly significant. Microbiological examination of samples were done and colony forming units were counted to assess the disinfection potential of photodynamic therapy. Scanning electron microscopic examination of samples was done to check penetration of bacteria's into deeper dentinal tubules.Results: On examination, there was a marked reduction in microbial growth after use of photodynamic therapy. On scanning electron microscopic examination, it was observed that there were less number of bacteria's in deeper dentinal tubules in case of PDT group as compared to control group. Conclusion:The results of the present study indicate that PDT can be effectively used during antimicrobial procedures along with conventional disinfection procedure for sterilization of root canals.

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Penetration ability of different irrigants into dentinal tubules

Cited by 234 publications

References 13 publications

Effect of final irrigation protocols on microhardness reduction and erosion of root canal dentin

Effect of final irrigation protocols on microhardness reduction and erosion of root canal dentin

Antimicrobial Effect and Surface Tension of Some Chelating Solutions with Added Surfactants

Assessment of Photodynamic Therapy (PDT) in Disinfection of Deeper Dentinal Tubules in a Root Canal System: An In Vitro Study

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