Penetrating Keratoplasty in the Rabbit

Khodadoust, Ali A.

doi:10.1016/0002-9394(68)92809-2

Cited by 36 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This conclusion was also supported by their experiments, in which they used the established techniques to study the rejection of full-thickness and lamellar corneal allografts in rabbits. 13 When a large penetrating corneal graft (8 mm) was placed centrally and the sutures were removed at the earliest possible time (10 days after grafting), vessels were found occasionally to reach the margin of the graft, but when a small penetrating corneal graft (5 mm) was employed, vascularisation of the recipi ent bed was limited to 1-2 mm near the limbus. These vessels became ghost vessels visible only by slit lamp examination.…”

Section: Analyses Of An 'Immunologically Privileged Site' In Sensitismentioning

confidence: 99%

The mechanisms of corneal graft failure in the rat

Katami¹

1995

Eye

View full text Add to dashboard Cite

SUMMARYThe success rate of corneal transplantation is very similar to that of other organ transplantations because corneal transplants can induce an allograft rejection similar to other organ transplants. Only the centre of the cornea can be considered an immunologically Medawar l -3 reported the histocompatibility relation ships between donor and recipient and the role of specific immunity in heterotopic skin graft rejection by using the anterior chamber of the eye. Medawa r described on the basis of the immunised rabbit model that, 'a skin homograft survives transplantation to the eye of a specifically and strongly immunised rabbit if, and only if, it remained unvascularised. The experiments provide further evidence for the general rule that transplantation immunity is the outcome of a systemic and not a local reaction; and they offer a rational explanation for the well-known clinical success of corneal homografts in human beings. ' However, the clinical demonstrations by Maume nee 4--6 that the corneal graft might be subject to immunological rejection, and that this process might account for a substantial proportion of those cases of late clouding of corneal allografts, prompted numer ous investigators to investigate each of the factors which might account for immunological privilege where it exists and for specific graft rejection when it occurs.Nowadays, recent improvements in microsurgery for orthotopic corneal transplantation in the rat (in

show abstract

Section: Analyses Of An 'Immunologically Privileged Site' In Sensitismentioning

confidence: 99%

The mechanisms of corneal graft failure in the rat

Katami¹

1995

Eye

View full text Add to dashboard Cite

show abstract

“…The fact that the systemic sensitization does not occur at all or is so weak that it cannot cause rejection of the grafted cornea speaks in fa vour of the corneal graft rejection reaction being a local immunological phenomenon [15]. The presence of T and B lymphocytes in the normal cornea also indicates that the cornea itself has its own immunological sys tem [14], It is very much possible that topi cally applied azathioprine as the solution has an immunosuppressive action on the immu nological system of the cornea and lymphoid structures of the limbus and adjacent tissue, causing immunologically competent cell pro liferation deceleration occurring after the an tigenic stimulation after corneal grafting.…”

Section: Discussionmentioning

confidence: 99%

Azathioprine and Cytarabine Applied Topically Prolong Corneal Graft Survival in Rabbits

Golubović¹

1991

Ophthalmic Res

View full text Add to dashboard Cite

Azathioprine and cytarabine applied topically as 0.1 % solution in the grafted rabbit eye significantly prolong corneal allograft survival time. Mean corneal graft survival time was 61 ± 27.76 days and 55 ± 26.79 days in the group treated with azathioprine and cytarabine, respectively, while mean corneal graft survival time in the control group of rabbits with no immunosuppressive therapy was 19 ± 4.47 days. After completion of the immunosuppressive treatment, the achieved azathioprine effect lasted longer than the cytarabine effect. Favourable azathioprine and cytarabine effects on corneal allograft survival in rabbits were obtained with no serious local and systemic side effects. Only moderate hyperemia of the palpebral and bulbar conjunctiva was recorded during the first days of treatment. No other side effects were observed.

show abstract

“…In preliminary trials protrusion of the bulbus for exposing the cornea as recom mended by Khodadoust [1968] lead to lens and vitreous expulsion. Perhaps our nega tive experience with protrusion was due to the fact that we operated on rather young animals which had extremely soft sclerae.…”

Section: Technique Of Corneal Grafting In Rabbitsmentioning

confidence: 99%

“…As stated by Khodadoust [ 1968] the prevention of clotting of aqueous humor is important. Since we induced no mydriasis the heparin could not reach the posterior chamber when applied topically.…”

Section: Technique Of Corneal Grafting In Rabbitsmentioning

confidence: 99%