Penapisan Aktivitas dan Senyawa Antidiabetes Ekstrak Air Daun Dandang Gendis (Clinacanthus nutans)

Abstract

An investigation of the antidiabetic activity of Aqueous Extract and its fractions from a different polarity solvent: ethanol, ethyl acetate, and n-hexane of Dandang Gendis Leaves (Clinacanthus nutans Lindau) on Mile SwissWebster Mice has been carried out. The effect of aqueous extract of dose of 50, 100, and 150 mg/kg BW was tested on glucose serum level of alloxan induced that mice. Its fractions were tested by glucose tolerance method. Aqueous extract (150 mg/kg BW) significantly decreased blood glucose serum level (pd"0,05) that had the same effect as oral glibenclamide (0,65 mg/kg BW) administrated once a day for 9 days. Aqueous extract that was fractionated has 5 fraction. Fraction of ethanol extract that was insoluble in ethyl acetate at a dose of 100 mg/kg BW was the most active fraction, that significantly (p<0,05) decreased blood glucose serum level of mice at glucose tolerance method of 18,4% in 3 hours after 2 g/kg BW of glucose administration. Thin layer chromatography (TLC) of that fraction ethanol 95%-chloroform 9:2 as eluent gave 5 spots under ultraviolet ray and showed spesific compounds by qualitative test. Spot 1 (steroid) gave Rf 0,14; spot 2 and spot 3 that gave Rf 0,53 and 0, 67 were flavonoid, spot 4 (triterpenoid) gave Rf 0,77; and spot 5 (chlorophyll) gave Rf 0,89. This research show that this plant can used for hipoglicemic traditional medicine.

Phenolic Rich Extract from Clinacanthus nutans Attenuates Hyperlipidemia‐Associated Oxidative Stress in Rats

“…This plant has been traditionally used in Asia to treat oxidative stress-related diseases such as CVD, diabetes, and various kinds of cancers. It is not surprising, therefore, that in recent years its extracts have been demonstrated to have blood glucose-lowering [ 11 ], antioxidant [ 12 , 13 ], antiproliferative [ 13 ], and anti-inflammatory effects [ 14 ]. Numerous bioactives have been demonstrated in C. nutans , including phenolics, flavonoids, β -sitosterol, stigmasterol, and chlorophyll derivatives [ 15 ].…”

Komponen Bioaktif dan Aktivitas Antioksidan Kerang Balelo (Conomurex sp.)

“…Senyawa steroid digunakan sebagai bahan baku obat dalam bidang pharmaceutical (Mukhriani, 2014). Nurulita et al (2008) menyatakan bahwa senyawa steroid fukosterol yang diisolasi dari biota laut bersifat tidak toksik dan berpotensi untuk menurunkan kolesterol, menurunkan gula dalam darah, mengatasi gangguan menstruasi, antibakteri dan antivirus…”

Analisis Rendemen dan Skrining Fitokimia Ekstrak Cacing Laut Eunice siciliensis

“…. Nurulita et al (2008) menyebutkan senyawa steroid merupakan komponen aktif yang telah digunakan untuk pengobatan penyakit diabetes, gangguan menstruasi, sebagai antibakteri dan antivirus.…”