Pembuatan Kitosan Dari Limbah Cangkang Udang Serta Aplikasinya Dalam Mereduksi Kolesterol Lemak Kambing

Hargono, Hargono; Abdullah, Abdullah; Sumantri, Indro

doi:10.14710/reaktor.12.1.53-57

Cited by 22 publications

(19 citation statements)

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“…In addition, the positive chitosan is able to react with the surface of negative anionic polymer, so it can facilitate the migration of inflammatory cells. 22,23 Based on several studies on chitosan as described above, it can be said that chitosan still needs to be developed since it has some potential functional characters for broad use in dentistry. Finally, it can be concluded that the deacetylation degree of chitosan derived from white shrimp shells could reach 85.165%.…”

Section: Methodsmentioning

confidence: 99%

Degrees of chitosan deacetylation from white shrimp shell waste as dental biomaterials

Sularsih

Yuliati

2012

Dent. J. (Majalah Kedokteran Gigi)

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Section: Methodsmentioning

confidence: 99%

Degrees of chitosan deacetylation from white shrimp shell waste as dental biomaterials

Sularsih

Yuliati

2012

Dent. J. (Majalah Kedokteran Gigi)

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“…Chitin was prepared from shrimp shell [9], and colloidal chitin was prepared by the addition of HCl [10]. The chitin broth for chitinase production was made of KH2PO4 (0.03%), K2HPO4 (0.07%), MgSO4.5H2O (0.05%), ZnSO4 (0.0001%), MnCl2 (0.0001%), starch (1.5%), yeast extract (0.15%) and colloidal chitin (1.5%).…”

Section: Medium and Inoculum Preparationmentioning

confidence: 99%

Optimization of Agitation Rate in Bioreactor Increases Chitinase Activity ofSerratia marcescensPT6

et al. 2020

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Serratia marcescens PT6 is a Gram-negative bacteria isolated from shrimp pond sediment that capable of producing chitinase. This study aimed to observe the effect of agitation rate on growth and chitinase activity of S. marcescens PT-6 in a bioreactor. The production of chitinase was done in 1.5 l bioreactor using colloidal chitin broth at the condition of pH 7, the temperature of 30°C, aeration of 0.04 vvm, and variation of agitation rate (200, 350, 500 rpm). Bacterial growth was measured by colonies counting in agar medium, while chitinase activity was measured by means of colorimetric every day for four days incubation. The results of ANOVA analysis show that the agitation rate had no effect on bacterial growth, but a significant effect (P<0.05) was observed on chitinase activity. The highest growth and chitinase activity were obtained at 200 rpm, with the highest chitinase activity of 0.006 ± 0.001 U/ml was at day-2. This study implies that the optimized agitation rate in the bioreactor increased the chitinase activity produced by S. marcescens PT-6.

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“…Pembuatan kitin mengacu pada Hargono & Sumantri (2008), sedangkan koloidal kitin mengacu pada Arnold & Solomon (1986).…”

Section: Metode Penelitianunclassified

Bioformation of N-Acetylglucosamine from Shrimp Shell Chitin by Serratia marcescens PT-6 Cultured in various pH and Temperature

Sari¹,

Isnaini²,

Puspita³

et al. 2017

JFS

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AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh pH dan suhu dalam pembentukan N-Asetilglukosamin (NAG) dari kitin cangkang udang oleh Serratia marcescens PT-6 yang difermentasi dalam media kitin cair. Parameter yang diuji dalam proses pembentukan NAG meliputi pertumbuhan bakteri, aktivitas kitinase (U/ml), serta konsentrasi NAG dalam medium (mg/ml). Pertumbuhan bakteri diukur berdasarkan kekeruhan (OD 600 ), sedangkan aktivitas kitinase dan konsentrasi NAG dalam medium dianalisis secara kuantitatif dengan metode kolorimetri. Variasi nilai pH yang dicobakan dalam media kitin cair adalah pH 5; 6; 7; 8, sedangkan variasi suhu yang dicobakan adalah 30°C; 37°C; dan 40°C. Hasil penelitian menunjukkan bahwa konsentrasi NAG maksimum yang dihasilkan oleh Serratia marcescens PT-6 sebesar 33,86 µg/ml pada hari ke-3 inkubasi, dengan kondisi pH 7 dan suhu 30°C. Aktivitas kitinase pada waktu yang sama mencapai nilai maksimum sebesar 0,002 U/ ml, sedangkan nilai OD 600 sebesar 0,42 menunjukkan bakteri berada pada fase logaritmik. Hasil penelitian ini menunjukkan bahwa Serratia marcescens PT-6 berpotensi untuk dikembangkan lebih lanjut dalam proses biokonversi NAG dari limbah cangkang udang secara enzimatis dengan memperhatikan kondisi kulturnya. Kata kunci: Kitinase, kitin, N-Asetilglukosamin, Serratia marcescens PT-6 AbstractThe study aimed to determine the effect of pH and temperature on N-Acetylglucosamine (NAG) formation from shrimp shell chitin by Serratia marcescens PT-6 fermented in chitin broth medium. Parameters examined on NAG formation include bacteria growth, chitinase activity (U/ml), and NAG concentration in medium (mg/ ml). Bacteria growth was measured by turbidity (OD 600 ), while chitinase activity and NAG concentration in the medium were analyzed quantitatively by colorimetric assay. The variation of initial pH examined in chitin broth medium was 5; 6; 7; 8, while temperature variation was 30°C; 37°C; dan 40°C. The results show that maximum concentration of NAG formed by Serratia marcescens PT-6 was 33.86 µg/ml on day-3 of fermentation, at pH 7 and temperature of 30°C. Chitinase activity on the same day was 0.002 U/ml, while OD 600 value of the culture was 0.42 indicating the bacteria were in the log phase. This research implies that Serratia marcescens PT-6 is potential to be optimized further for the bioconversion process of NAG from shrimp shell waste through an enzymatic method by modifying its culture condition.

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Pembuatan Kitosan Dari Limbah Cangkang Udang Serta Aplikasinya Dalam Mereduksi Kolesterol Lemak Kambing

Cited by 22 publications

References 0 publications

Degrees of chitosan deacetylation from white shrimp shell waste as dental biomaterials

Degrees of chitosan deacetylation from white shrimp shell waste as dental biomaterials

Optimization of Agitation Rate in Bioreactor Increases Chitinase Activity ofSerratia marcescensPT6

Bioformation of N-Acetylglucosamine from Shrimp Shell Chitin by Serratia marcescens PT-6 Cultured in various pH and Temperature

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