Pehuén (Araucaria araucana) seed residues are a valuable source of natural antioxidants with nutraceutical, chemoprotective and metal corrosion-inhibiting properties

Gallia, María Celeste; Bachmeier, Evelin; Ferrari, Alejandro; Queralt, I.; Mazzeo, M; Bongiovanni, Guillermina A.

doi:10.1016/j.bioorg.2020.104175

Cited by 10 publications

(5 citation statements)

References 58 publications

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“…Considering the nutraceutical potential of piñón callus, cytotoxicity of the extract was determined. As expected, no early cytotoxic effects were found in VERO cells exposed to piñón callus extract, such as it was reported by Gallia et al [ 15 ] with piñón embryo, endosperm, and coat. This results agree with the historical medicinal and nutritional uses of these seeds by regional communities [ 12 ] and other research about cytoprotective effects of phenolic extracts of species of the genus Araucaria [ 34 , 35 ].…”

Section: Discussionsupporting

confidence: 84%

“…This result suggests that the biomass of piñones produced in vitro would be an interesting nutraceutical candidate for human consumption. Additionally, our previous results [ 15 ] showed that pulp (edible part composed by embryo and endosperm; mean weight 1.8 g per piñón) of one piñón contains 1.55 mg AAE, while 0.208 mg AAE can be provided from callus of one embryo. However, 540 days (18 months) are necessary to obtain one matured seed, while in vitro production is continuous (24/7).…”

Section: Discussionmentioning

confidence: 99%

“…African green monkey ( Cercopithecus aethiops ) kidney cells (VERO cell line ATCC n° CCL-81) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) completed with 10% fetal bovine serum, 100 U/ml penicillin-G, and 40 μg/ml gentamycin sulfate, incubated at 37 °C in a 5% CO 2 atmosphere [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…However, plants need from 25 to 30 years for optimal development and sexual maturity, although in xeric environment as Argentinian Patagonia sexual differentiation would occur at 75–100 years of age [ 14 ]. Piñones are eaten raw, boiled, and toasted and contain antioxidants, polyphenols, flavonoids, and oligoelements like P, K, Mn, Fe, Cu, and Zn [ 15 ]. In view of this and many other reasons, A. araucana populations have suffered unsustainable exploitation and significant reduction in their distribution area.…”

Section: Introductionmentioning

confidence: 99%

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Sustainable and efficient protocols for in vitro germination and antioxidants production from seeds of the endangered species Araucaria araucana

Gallia

Sarto

Bongiovanni

2021

Journal of Genetic Engineering and Biotechnology

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Background The Pehuén or Monkey puzzle tree (Araucaria araucana) is an evergreen coniferous tree, which has been historically used for social, medicinal, and nutritional purposes. We have recently showed the value of A. araucana seeds as a rich source of micronutrients and antioxidants. This endemic species present in a reduced area in Argentina and Chile is endangered because of the low germination rate and the overexploitation of its edible seeds. Thus, the massive extraction of its seeds is ecologically non-viable resulting in limited availability of its active metabolites. However, biotechnological approaches are attractive strategies of production of valuable metabolites and healthy specimens of endangered plants. The aim of this work was to develop a protocol for in vitro production of antioxidants derived from A. araucana seeds and to obtain healthy plants by optimized seed germination. Results Calli of Pehuén seeds were induced in Murashige and Skoog medium with different combinations of auxins and cytokinins, in light and dark conditions. Callus from embryonic axes developed in medium with 1 mg/l α-naphthaleneacetic acid and 1.5 mg/l 6-benzylaminopurine in light conditions had efficient biomass production, antioxidant activity, high phenolic, and flavonoid content and no cytotoxicity on mammalian cells. Additionally, 100 % germination was obtained in vitro and healthy plants were acclimatized to non-sterile conditions. Conclusion In conclusion, in vitro culture of A. araucana could provide new and sustainable options for production of its valuable metabolites with possible therapeutic and nutritional uses. Also, optimized plant germination and acclimatization of endangered species can contribute to the preservation of pristine environments.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sustainable and efficient protocols for in vitro germination and antioxidants production from seeds of the endangered species Araucaria araucana

Gallia

Sarto

Bongiovanni

2021

Journal of Genetic Engineering and Biotechnology

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“…21 Ensayos previos de nuestro laboratorio a nivel clínico y experimental han demostrado en otras drogas antineoplásicas no solo alteración de los niveles de lipoperoxidación a través de la medición de malondialdehido, sino también de la batería antioxidante enzimática y no enzimática por medio de la determinación de la actividad de Superóxido Dismutasa y la determinación de los niveles de Ácido Úrico respectivamente. [22][23] En el presente trabajo, Carboplatino en la dosis ensayada de 100mg/Kg de peso corporal provocó un aumento significativo de la lipoperoxidación en glándula submandibular de ratas. Esto podría desencadenar un incorrecto funcionamiento de los mecanismos de secreción salival de la glándula submandibular responsable del 70% de la secreción basal.…”

Section: Discussionunclassified

Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats

Bachmeier¹,

Goitea²,

Linares³

et al. 2021

RevFacOdont

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The objective of the present work was to evaluate the effect of carboplatin (Cp) on submandibular gland homogenate of Wistar rats through the determination of malondialdehyde levels, as the main end product of lipoperoxidation, in an experimental model.Sixteen three-month-old male Wistar rats were used, housed in individual cages, with controlled temperature and lighting and free diet. A completely randomized design was used and two experimental groups were established: 1) Control (C), administering an intraperitoneal dose of saline solution for one day, n: 8, 2) Animals treated with carboplatin (Cp) applying a dose i.p. of 100 mg / Kg of body weight for one day, n: 8. The animals were fasted for 24 hours and subsequently anesthetized. Then both submandibular glands were removed. Malondialdehyde levels were analyzed in submandibular gland homogenatein both experimental groups. Variations between the groups analyzed were evaluated using the Student's t test for paired samples, setting a p-value <0.05 for statistical significance. Project approved by CICUAL. Faculty of Medical Sciences (UNC).The group of Rats Co showed a concentration of 7.32± 0.48μmol / mg of gland. The Cp group had a concentration of 12.57 ± 0.71 μmol / mg of gland, expressing a significant decrease compared to the control group p <0.0006. Cp at the dose tested would cause a decrease in lipoperoxidation in the submandibular gland of rats. Possibly the glandular antioxidant battery would neutralize the oxidative stress of acinar cells. These results suggest a future evaluation of superoxide dismutase (SOD) activity and uric acid (UA) levels

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Forest-Based Edible Seeds and Nuts for Health Care and Disease Control

Godeto

Bachheti

Husen

et al. 2021

Non-Timber Forest Products

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Pehuén (Araucaria araucana) seed residues are a valuable source of natural antioxidants with nutraceutical, chemoprotective and metal corrosion-inhibiting properties

Cited by 10 publications

References 58 publications

Sustainable and efficient protocols for in vitro germination and antioxidants production from seeds of the endangered species Araucaria araucana

Sustainable and efficient protocols for in vitro germination and antioxidants production from seeds of the endangered species Araucaria araucana

Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats

Forest-Based Edible Seeds and Nuts for Health Care and Disease Control

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