Pegylated granulocyte colony-stimulating factor mobilizes CD34+ cells with different stem and progenitor subsets and distinct functional properties in comparison with unconjugated granulocyte colony-stimulating factor

Abstract

BackgroundPegylated granulocyte colony-stimulating factor (G-CSF) has recently been introduced as a new compound for mobilization of CD34 + hematopoietic stem and progenitor cells. In this study, we compared the molecular and functional characteristics of CD34 + cells mobilized by pegylated G-CSF with those mobilized by unconjugated G-CSF.

Mobilization of Progenitor Cells into the Blood by Immobilized Granulocytic Colony-Stimulating Factor

“…Our results are in complete agreement with published data indicating stimulation of hemopoietic precursors release into peripheral blood under the effect of G-CSF, in parallel with the increase in functional activity of CFU-GM [1,2,8,13].…”

Low RPS14 expression is common in myelodysplastic syndromes without 5q- aberration and defines a subgroup of patients with prolonged survival

“…6 RNA was purified as described 6 and samples were stored at -80°C. For detection and quantification of mRNA levels of RPS14 and GAPDH the LightCycler 1.2 technology (Roche, Mannheim, Germany) was used.…”

Allogeneic hematopoietic stem cell donation—standardized assessment of donor outcome data: A consensus statement from the Worldwide Network for Blood and Marrow Transplantation (WBMT)

“…It is important to realize that G-CSF, PEG-G-CSF and CXCR-4 antagonists recruit different cell populations according to global gene and mRNA expression levels. [34][35][36] Finally, it is possible that biosimilars of G-CSF and EPO will also be applied in healthy donors although recent statements from the European Group for Blood and Marrow Transplantation (EBMT) and WMDA do not recommend it outside of the context of well set up safety studies. This emphasizes the need to include all current mobilizing agents as well as any new agents that will be introduced into clinical practice in the future in a prospective follow-up.…”