PEG–PLGA nanoparticles for encapsulating ciprofloxacin

Watcharadulyarat, Natsorn; Rattanatayarom, Monthira; Ruangsawasdi, Nisarat; Patikarnmonthon, Nisa

doi:10.1038/s41598-023-27500-y

Cited by 8 publications

(7 citation statements)

References 57 publications

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“…67 A similar increase in size was noticed too when drugs were encapsulated in PEG− PLGA-based nanoparticles when compared with drug-free nanoparticles. 50 The R h of ZIF particles was found to be 450 ± 40 nm, close to the literature-reported value found by Yahia et al and others. 68 The encapsulation of ZIF increased the overall size of the polymeric particles.…”

Section: Preparation and Characterization Of Lysozyme-encapsulated Na...supporting

confidence: 89%

“…Standard curves were prepared using BSA (Sigma-Aldrich) in concentrations ranging from 0.2 to 1 mg/mL. Lysozyme encapsulation efficiency was calculated according to eq . , Details of nanoparticle characterization are presented in the Supporting Information (SI). % encapsulation efficiency 0.25em ( % EE ) = amount 0.25em of 0.25em protein 0.25em in 0.25em NPs initial 0.25em amount 0.25em of 0.25em protein 0.25em in 0.25em water × 100 …”

Section: Methodsmentioning

confidence: 99%

“…The PEG−PLGA nanoparticles were spherical, with a smaller mean diameter than lysozyme-encapsulated PEG−PLGA nanoparticles, supporting the DLS analysis and in accordance with the literature. 50,71 The TEM image of ZIFs (Figure 2E) showed a hexagonal morphology inside a PEG− PLGA nanoparticle. This hexagonal shape is formed by distorting the water-driven hydrogen-bonding interactions between the monodentate methylimidazole in one direction and the free imidazole in another direction.…”

Section: Preparation and Characterization Of Lysozyme-encapsulated Na...mentioning

confidence: 99%

“…Lysozyme encapsulation efficiency was calculated according to eq 1. 50,51 Details of nanoparticle characterization are presented in the Supporting Information (SI).…”

Section: Nanoscale Characterization Ofmentioning

confidence: 99%

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Metal–Organic Framework Induced Stabilization of Proteins in Polymeric Nanoparticles

Khan,

Armstrong,

Lenertz

et al. 2024

ACS Appl. Mater. Interfaces

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Developing protein confinement platforms is an attractive research area that not only promotes protein delivery but also can result in artificial environment mimicking of the cellular one, impacting both the controlled release of proteins and the fundamental protein biophysics. Polymeric nanoparticles (PNPs) are attractive platforms to confine proteins due to their superior biocompatibility, low cytotoxicity, and controllable release under external stimuli. However, loading proteins into PNPs can be challenging due to the potential protein structural perturbation upon contacting the interior of PNPs. In this work, we developed a novel approach to encapsulate proteins in PNPs with the assistance of the zeolitic imidazolate framework (ZIF). Here, ZIF offers an additional protection layer to the target protein by forming the protein@ZIF composite via aqueous-phase cocrystallization. We demonstrated our platform using a model protein, lysozyme, and a widely studied PNP composed of poly(ethylene glycol)poly(lactic-co-glycolic acid) (PEG−PLGA). A comprehensive study via standard loading and release tests as well as various spectroscopic techniques was carried out on lysozyme loaded onto PEG−PLGA with and without ZIF protection. As compared with the direct protein encapsulation, an additional layer with ZIF prior to loading offered enhanced loading capacity, reduced leaching, especially in the initial stage, led to slower release kinetics, and reduced secondary structural perturbation. Meanwhile, the function, cytotoxicity, and cellular uptake of proteins encapsulated within the ZIF-bound systems are decent. Our results demonstrated the use of ZIF in assisting in protein encapsulation in PNPs and established the basis for developing more sophisticated protein encapsulation platforms using a combination of materials of diverse molecular architectures and disciplines. As such, we anticipate that the protein-encapsulated ZIF systems will serve as future polymer protein confinement and delivery platforms for both fundamental biophysics and biochemistry research and biomedical applications where protein delivery is needed to support therapeutics and/or nutrients.

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Section: Preparation and Characterization Of Lysozyme-encapsulated Na...supporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Preparation and Characterization Of Lysozyme-encapsulated Na...mentioning

confidence: 99%

“…Lysozyme encapsulation efficiency was calculated according to eq 1. 50,51 Details of nanoparticle characterization are presented in the Supporting Information (SI).…”

Section: Nanoscale Characterization Ofmentioning

confidence: 99%

See 2 more Smart Citations

Metal–Organic Framework Induced Stabilization of Proteins in Polymeric Nanoparticles

Khan,

Armstrong,

Lenertz

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…The clinic bacteria E. coli (BL21) and drug-resistant MRSA (ATCC43300) were chosen for the labeling experiment utilizing DAPI and PI, which would further confirm whether PAC-NPs would indeed destroy the membranes’ structure. Thus, following the previously reported double staining methods, 49 the bacteria were stained utilizing DAPI and PI before and after treatment with PAC-NPs, respectively.…”

Section: Resultsmentioning

confidence: 99%