PEG hydrogel containing calcium-releasing particles and mesenchymal stromal cells promote vessel maturation

Nieva, Claudia; Weaver, Jessica D.; Clark, Amy Y.; Clift, Douglas A.; Pérez‐Amodio, Soledad; Castaño, Óscar; Zhou, Dennis W.; Garcı́a, Andrés J.; Engel, Elisabeth

doi:10.1016/j.actbio.2017.12.009

Cited by 20 publications

(13 citation statements)

References 74 publications

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“…8,11,12 Hydrogels can increase local cell retention and engraftment at the injection site compared to traditional liquid injections or infusion by acting as local niches to physically hold cells in place. 17 The development of shear-thinning hydrogels has enabled quicker, less invasive administration procedures by injection through a needle. 14 The benefits of encapsulating hMSCs in hydrogels have been shown in multiple clinical studies targeting an array of indications.…”

Section: Funding Informationmentioning

confidence: 99%

“…10,15,16 Yet, crosslinked hydrogels containing cells often require invasive implantation procedures. 17 The development of shear-thinning hydrogels has enabled quicker, less invasive administration procedures by injection through a needle. 16,[18][19][20][21][22][23] Injectable hydrogels enhance cell viability during the injection process compared to liquid vehicles by alleviating the mechanical forces cells experience when traveling through small diameter needles.…”

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confidence: 99%

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Injectable supramolecular polymer–nanoparticle hydrogels enhance human mesenchymal stem cell delivery

Grosskopf

Roth

Smith

et al. 2019

Bioengineering & Transla Med

121

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Stem cell therapies have emerged as promising treatments for injuries and diseases in regenerative medicine. Yet, delivering stem cells therapeutically can be complicated by invasive administration techniques, heterogeneity in the injection media, and/or poor cell retention at the injection site. Despite these issues, traditional administration protocols using bolus injections in a saline solution or surgical implants of cellladen hydrogels have highlighted the promise of cell administration as a treatment strategy. To address these limitations, we have designed an injectable polymernanoparticle (PNP) hydrogel platform exploiting multivalent, noncovalent interactions between modified biopolymers and biodegradable nanoparticles for encapsulation and delivery of human mesenchymal stem cells (hMSCs). hMSC-based therapies have shown promise due to their broad differentiation capacities and production of therapeutic paracrine signaling molecules. In this work, the fundamental hydrogel mechanical properties that enhance hMSC delivery processes are elucidated using basic in vitro models. Further, in vivo studies in immunocompetent mice reveal that PNP hydrogels enhance hMSC retention at the injection site and retain administered hMSCs locally for upwards of 2 weeks. Through both in vitro and in vivo experiments, we demonstrate a novel scalable, synthetic, and biodegradable hydrogel system that overcomes current limitations and enables effective cell delivery. K E Y W O R D Scell delivery, human mesenchymal stem cell, hydrogel, injectable

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Section: Funding Informationmentioning

confidence: 99%

mentioning

confidence: 99%

Injectable supramolecular polymer–nanoparticle hydrogels enhance human mesenchymal stem cell delivery

Grosskopf

Roth

Smith

et al. 2019

Bioengineering & Transla Med

121

View full text Add to dashboard Cite

show abstract

“…The same sol–gel‐derived phosphate glass‐ceramic nanoparticles were also used in combination with a hydrogel of PEG‐maleimide seeded with human MSCs. [ 84 ] The composite was tested in an ex ovo CAM assay as well as an in vivo mouse model where the hydrogels were implanted in the epididymal fat pad. The addition of the nanoparticles enhanced blood vessel formation and maturation in vivo through the improved release of angiogenetic factors and greater extent of cell implant survival.…”

Section: Bioactive Sol–gel Phosphate‐based Glasses (Sgpgs)mentioning

confidence: 99%

A Review of Phosphate and Borate Sol–Gel Glasses for Biomedical Applications

Lepry

Nazhat

2021

Advanced NanoBiomed Research

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The sol–gel processing method revolutionizes the biomedical materials field, allowing for the customized creation of nano‐ and porous materials to help treat the toughest challenges in human health. However, this process and the subsequently produced materials have mostly been based on silica, particularly in terms of biomedical glasses. Yet, within the last two decades, there has been increased interest in silica‐free glass chemistries, such as those based on borate or phosphate. Attributable to their distinct properties which allows for linear and complete degradation, these glass compositions have shown great promise for both hard and soft tissue engineering applications, albeit with only a limited number of studies on glasses created through the sol–gel process. Therefore, this review provides an overview of the advancement of nonsilica sol–gel glasses, by focusing on borate and phosphate chemistries, for biomedical applications. A comprehensive review of these materials, including the challenges in processing as well as the current uses and future potential, is discussed.

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“…44,45 In previous studies we have demonstrated that calcium-releasing particles stimulate the release of angiogenic growth factors in endothelial progenitor cells 46 and mesenchymal stromal cells. 47 Hence, it is possible that the improved wound healing effects observed by the SG5-mats might be attributable, in part, to a stimulation of angiogenic factor production that resulted in increased angiogenesis. In chronic wounds, the formation of new blood vessels is limited by several factors, including diabetes and obesity.…”

Section: ____-____0 ____+mentioning

confidence: 99%

Polymeric Composite Dressings Containing Calcium-Releasing Nanoparticles Accelerate Wound Healing in Diabetic Mice

Pérez‐Amodio

Rubio

Vila

et al. 2021

Advances in Wound Care

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Objective: Wound healing is a complex process that involves the interaction between different cell types and bioactive factors. Impaired wound healing is characterized by a loss in synchronization of these interactions, resulting in nonhealing chronic wounds. Chronic wounds are a socioeconomic burden, one of the most prominent clinical manifestations of diabetes, however, they lack satisfactory treatment options. The objective of this study was to develop polymeric composites that deliver ions having wound healing properties and evaluate its performance using a pressure ulcer model in diabetic mice. Approach: To develop a polymeric composite wound dressing containing ionreleasing nanoparticles for chronic wound healing. This composite was chemically and physically characterized and evaluated using a pressure ulcer wound model in diabetic (db/db) mice to explore their potential as novel wound dressing. Results: This dressing exhibits a controlled ion release and a good in vitro bioactivity. The polymeric composite dressing treatment stimulates angiogenesis, collagen synthesis, granulation tissue formation, and accelerates wound closure of ischemic wounds created in diabetic mice. In addition, the performance of the newly designed composite is remarkably better than a commercially available dressing frequently used for the treatment of lowexuding chronic wounds. Innovation: The developed nanoplatforms are cell-and growth factor free and control the host microenvironment resulting in enhanced wound healing. These nanoplatforms are available by cost-effective synthesis with a defined composition, offering an additional advantage in potential clinical application. Conclusion:Based on the obtained results, these polymeric composites offer an optimum approach for chronic wound healing without adding cells or external biological factors.

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PEG hydrogel containing calcium-releasing particles and mesenchymal stromal cells promote vessel maturation

Cited by 20 publications

References 74 publications

Injectable supramolecular polymer–nanoparticle hydrogels enhance human mesenchymal stem cell delivery

Injectable supramolecular polymer–nanoparticle hydrogels enhance human mesenchymal stem cell delivery

A Review of Phosphate and Borate Sol–Gel Glasses for Biomedical Applications

Polymeric Composite Dressings Containing Calcium-Releasing Nanoparticles Accelerate Wound Healing in Diabetic Mice

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