PEG-dialdehyde-the new cross-linking agent for collagen/elastin hydrogels

Skopińska-Wiśniewska, Joanna; Bajek, Anna; Maj, Małgorzata; Sionkowska, Alina

doi:10.1002/pat.3970

Cited by 7 publications

(5 citation statements)

References 36 publications

(46 reference statements)

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“…Collagen hydrogels were proposed for 3D multicellular microfluidic chip construction applied in an in vitro skin model which can function as a more physiologically realistic platform for testing skin reactions to cosmetic products and drugs [16]. Collagen-based hydrogels can be cross-linked with EDC/ NHS, starch dialdehyde, tannic acid, squaric acid, PEG-dialdehyde UV radiation and many other cross-linking agents [17][18][19][20][21].…”

Section: Collagen In Cosmeticsmentioning

confidence: 99%

Biopolymers for hydrogels in cosmetics: review

Mitura

Sionkowska

Jaiswal³

2020

J Mater Sci: Mater Med

186

114

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Hydrogels are cross-linked networks of macromolecular compounds characterized by high water absorption capacity. Such materials find a wide range of biomedical applications. Several polymeric hydrogels can also be used in cosmetics. Herein, the structure, properties and selected applications of hydrogels in cosmetics are discussed in general. Detailed examples from scientific literature are also shown. In this review paper, most common biopolymers used in cosmetics are presented in detail together with issues related to skin treatment and hair conditioning. Hydrogels based on collagen, chitosan, hyaluronic acid, and other polysaccharides have been characterized. New trends in the preparation of hydrogels based on biopolymer blends as well as bigels have been shown. Moreover, biopolymer hydrogels employment in encapsulation has been mentioned.

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Section: Collagen In Cosmeticsmentioning

confidence: 99%

Biopolymers for hydrogels in cosmetics: review

Mitura

Sionkowska

Jaiswal³

2020

J Mater Sci: Mater Med

186

114

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“…This may also be the cause of low cytotoxicity results obtained for our nontreated hAM and, as a consequence, also for cross-linked materials. Our previous works showed that the cross-linking factors used in this research support cells' attachment and growth when used for collagen, elastin, or gelatin crosslinking, and they can be applied for modification of biomaterials for tissue regeneration and used as a safe alternative for popular cross-linking agents [18][19][20]27]. We expect a similar effect in the case of hAM modification, but further studies should be conducted considering how the amniotic membrane is stored.…”

Section: Discussionmentioning

confidence: 64%

“…This is proved by the increase in the thermal degradation temperature of cross-linked materials, as well as the changes observed in the FTIR spectra. The increase in Amide I and Amide II intensity indicates the formation of new amide bonds as a result of reactions between aldehyde groups of DAS, PEG-A, and amino groups of hAM [18,19], and also mediated by EDC/NHS and PEG-NHS [4]. As a result, the mechanical strength of cross-linked human amniotic membranes is improved.…”

Section: Discussionmentioning

confidence: 99%

“…Also, PEG-A and PEG-NHS are amine-reactive compounds. They are formed as a result of modification of the hydroxyl end groups of the polyethylene glycol chain-the water-soluble, neutral, and biocompatible polymer widely used in pharmacy [18,19]. We also used squaric acid (SQ) (3,4-dihydroxy 3-cyclobutene 1,2-dione), which is a strong aromatic acid.…”

Section: Introductionmentioning

confidence: 99%

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Modification of the Human Amniotic Membrane Using Different Cross-Linking Agents as a Promising Tool for Regenerative Medicine

Skopinska-Wisniewska,

Michalak,

Tworkiewicz

et al. 2023

Materials

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Human amniotic membranes (hAMs) obtained during cesarean sections have proven to be clinically useful as an interesting biomaterial in a wide range of tissue engineering applications such as ocular surface reconstruction, burn treatments, chronic wounds, or bedsore ulcers. It presents antimicrobial properties, promotes epithelization, reduces inflammation and angiogenesis, contains growth factors, and constitutes the reservoir of stem cells. However, variability in hAM stiffness and its fast degradation offers an explanation for the poor clinical applications and reproducibility. In addition, the preparatory method of hAM for clinical use can affect its mechanical properties, and these differences can influence its application. As a directly applied biomaterial, the hAM should be available in a ready-to-use manner in clinical settings. In the present study, we performed an analysis to improve the mechanical properties of hAM by the addition of various reagents used as protein cross-linkers: EDC/NHS, PEG-dialdehyde, PEG-NHS, dialdehyde starch, and squaric acid. The effect of hAM modification using different cross-linking agents was determined via infrared spectroscopy, thermal analyses, mechanical properties analyses, enzymatic degradation, and cytotoxicity tests. The use of PEG-dialdehyde, PEG-NHS, dialdehyde starch, and squaric acid increases the mechanical strength and elongation at the breaking point of hAM, while the addition of EDC/NHS results in material stiffening and shrinkage. Also, the thermal stability and degradation resistance were evaluated, demonstrating higher values after cross-linking. Overall, these results suggest that modification of human amniotic membrane by various reagents used as protein cross-linkers may make it easier to use hAM in clinical applications, and the presented study is a step forward in the standardization of the hAM preparation method.

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“…Other natural macromolecules can be introduced to obtain COL‐based composites with better properties. [ 6 ]…”

Section: Introductionmentioning

confidence: 99%

Nanofiber formation of self‐crosslinking dialdehyde carboxymethyl cellulose/collagen composites

Tian

Wang

2021

Polymer Engineering & Sci

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Self‐crosslinking dialdehyde carboxymethyl cellulose (DCMC)/collagen (COL) composites were prepared by blending DCMC and COL at different ratios, and the effect of DCMC amounts on composite nanofiber formation and stability were investigated. The more was the DCMC amount, the higher became the cross‐linking degree, thus demonstrating that self‐crosslinking system was successfully constructed. At low amount of DCMC, self‐assembled DCMC/COL composite exhibited typical nanofiber structure similar to COL nanofibers, and the mean diameter of nanofibers increased from 68 ± 12 to 84 ± 15 nm; additionally, the final turbidity of DCMC/COL composite was higher than that of COL, reflecting more nanofibers formed. We conjectured that nanofiber formation was promoted via adding a small amount of DCMC. However, the inhibition of nanofiber formation was detected by reductions in turbidity at 0.3:1 < DCMC/COL ≤ 2.5:1, and the mean diameter of nanofibers decreased to 54 ± 13 nm at a DCMC/COL ratio of 2.5:1. Meanwhile, crosslinking occurred again between adjacent nanofibers during self‐assembly process; therefore, partial nanofibers presented in pairs. At a DCMC/COL ratio of 5:1, nanofiber formation was completely inhibited and DCMC/COL composite consisted of aggregates. In summary, nanofiber formation was first promoted and then inhibited with increased DCMC amounts. Furthermore, low‐ and high‐temperature stability of DCMC/COL nanofibers was better than those of COL nanofibers due to the formation of crosslinks.

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PEG-dialdehyde-the new cross-linking agent for collagen/elastin hydrogels

Cited by 7 publications

References 36 publications

Biopolymers for hydrogels in cosmetics: review

Biopolymers for hydrogels in cosmetics: review

Modification of the Human Amniotic Membrane Using Different Cross-Linking Agents as a Promising Tool for Regenerative Medicine

Nanofiber formation of self‐crosslinking dialdehyde carboxymethyl cellulose/collagen composites

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