PEG conjugation of a near-infrared fluorescent probe for noninvasive dual imaging of lung deposition and gene expression by pulmonary gene delivery

Okuda, Tomoyuki; Kobayashi, Yuko; Yanamoto, Shinya; Okamoto, Hirokazu

doi:10.3109/1061186x.2012.722639

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…In this study, we prepared conventional gel formulations with a fluorescent drug to investigate the fate of the drug after administering them intra-articularly. We traced the fate of indocyanine green (ICG), which is clinically used as an agent for liver/circulatory function tests and has also been used in pharmacokinetic studies as a fluorescent substance in laboratories, 7) after intraarticular administration in rats. ICG is highly soluble in water and is suitable as a tracer by fluorescence imaging.…”

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confidence: 99%

Development of Intra-knee Joint Sustained-Release Gel Formulation and Evaluation of Its Pharmacological Efficiency in Rats

Noda

Okuda

Ban

et al. 2017

Biological & Pharmaceutical Bulletin

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In the development of a drug for intra-articular administration, a sustained-release formulation is desirable since it is difficult to sustain the effects of conventional injections due to fast drug leakage from the joint cavity. In this study, we prepared sustained release gel formulations for intra-articular administration containing indocyanine green (ICG) as a model drug to follow its fate after intra-articular administration in rats with in-vivo imaging system (IVIS). ICG administered as an aqueous solution leaked from the joint cavity in a short time and was excreted out of the body within a day. On the other hand, ICG in the sustained-release formulations was retained and released in the joint cavity for a week. Next, we prepared a sustained-release formulation with hyaluronic acid (HA) as the gel base containing a pain-relief drug (Drug A). We had administered it and other formulations into the rat knee where we injected bradykinin to evaluate their walking distance after 1 and 3 d. The effect of an aqueous solution of Drug A disappeared on day 3. The HA gel formulation without Drug A was more effective than the aqueous solution. The HA gel formulation with Drug A was the most effective; the walking distance was about 85% of the baseline on day 3. This study showed that the gel formulations were effective to sustain the release of a drug in the knee joint, and that the combination of a pain-relief drug with HA gel was effective to improve the mobility of the acute pain model rats.

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mentioning

confidence: 99%

Development of Intra-knee Joint Sustained-Release Gel Formulation and Evaluation of Its Pharmacological Efficiency in Rats

Noda

Okuda

Ban

et al. 2017

Biological & Pharmaceutical Bulletin

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“…It should be noted that many other biocompatible, water soluble and biodegradable polymers have been developed as in vivo imaging probes 58 . For example, Poly(ethylene glycol) 59 , Poly (L-glutamic acid) 60 and N-(2-Hydroxypropyl) methacrylamide 61 with various functional groups have been used to fabricate probes with different targeting and therapeutic moieties. It is possible the FR-targeting probes can be made using different polymer carriers.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic Acid-Based Optical Probe for the Diagnosis of Human Osteoarthritic Cartilage

Hakamivala

Huang

et al. 2018

Nanotheranostics

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Osteoarthritis is typically caused by cartilage injury, followed by localized inflammatory responses and tissue deterioration. Early treatment of osteoarthritis is often impossible due to the lack of diagnostic options. Recent studies have supported that different imaging probes can be used for arthritis detection in mice. However, none of these diagnostic tools have been tested on human articular cartilage. To fill this gap, an optical imaging probe was developed to target activated macrophages and the accumulation of imaging probes on tissue was used to assess the severity of human osteoarthritis.Methods: The probe was fabricated using hyaluronic acid (HA) particles conjugated with near-infrared dye and folic acid (FA). The ability of the FA-HA probes to detect activated macrophages and quantify cartilage injury was evaluated using a cell culture model in vitro and human osteoarthritic cartilage explants ex vivo.Results: Our cell study results supported that the FA-HA probes are cell compatible (up to 0.5mg/mL) and can detect activated macrophages in 30 minutes. Using human articular cartilage, we verified the existence of activated macrophages on osteoarthritic cartilage with highly up-regulated expression of folate receptors (~13 folds by comparison with healthy control). In addition, we found that FA-HA probes had higher binding amounts (~3 folds) to osteoarthritic tissue than healthy ones. Histological analyses confirmed that there was a strong linear relationship (R=0.933) between the fluorescent intensity of tissue-associated probe and the extent of folate receptors on osteoarthritic cartilage. Finally, the co-localization of the imaging probe, folate receptors and cartilage degeneration on the tissue sections indicated the extraordinary accuracy and efficiency of this osteoarthritis diagnostic probe.Conclusions: Our results support the probe as an effective diagnostic tool to detect the area and severity of osteoarthritic human articular cartilage.

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“…A lung deposition and gene expression correlation study found that after 60 minutes, target gene expression was significant. 44 This study therefore highlights the importance of using a heptamethine dye, as opposed to an ICG dye, for improved antitumor efficacy when using a gene delivery strategy.…”

Section: Cancer Therapy Using Heptamethine Nirf Nanocomplexesmentioning

confidence: 93%

NIRFHeptamethine Cyanine Dye Nanocomplexes for Multi Modal Theranosis of Tumors

Thomas

Jeong

2017

Chonnam Med J

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Heptamethine cyanine dyes are categorized as a class of near infrared fluorescent (NIRF) dyes which have been discovered to have tumor targeting and accumulation capability. This unique feature of NIRF dye makes it a promising candidate for imaging, targeted therapy and also as a drug delivery vehicle for various types of cancers. The favored uptake of dyes only in cancer cells is facilitated by several factors which include organic anion-transporting polypeptides, high mitochondrial membrane potential and tumor hypoxia in cancer cells. Currently nanotechnology has opened possibilities for multimodal or multifunctional strategies for cancer treatment. Including heptamethine cyanine dyes in nanoparticle based delivery systems have generally improved its theranostic ability by several fold owing to the multiple functionalities and structural features of heptamethine dyes. For this reason, nanocomplexes with NIRF heptamethine cyanine dye probe are preferred over non-targeting dyes such as indo cyanine green (ICG). This review sums up current trends and progress in NIRF heptamethine cyanine dye, including dye properties, multifunctional imaging and therapeutic applications in cancer.

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PEG conjugation of a near-infrared fluorescent probe for noninvasive dual imaging of lung deposition and gene expression by pulmonary gene delivery

Cited by 7 publications

References 32 publications

Development of Intra-knee Joint Sustained-Release Gel Formulation and Evaluation of Its Pharmacological Efficiency in Rats

Development of Intra-knee Joint Sustained-Release Gel Formulation and Evaluation of Its Pharmacological Efficiency in Rats

Hyaluronic Acid-Based Optical Probe for the Diagnosis of Human Osteoarthritic Cartilage

NIRFHeptamethine Cyanine Dye Nanocomplexes for Multi Modal Theranosis of Tumors

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