Peer Reviewed: SPR of Ultrathin Organic Films

Frutos, Anthony G.; Corn, Robert M.

doi:10.1021/ac981909r

Cited by 103 publications

(98 citation statements)

References 30 publications

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“…SPCE displays similar properties of SPR 17,18 except that the surface plasmons are created by interactions with the excited fluorophores. These plasmons then emit into the glass prism, which is possible because the wavevector of the plasmons can be matched with far-field radiation according to eq 6.…”

Section: Theorymentioning

confidence: 95%

“…17,18 In reality SPR does not occur only at a single angle but over a relatively narrow range of angles determined by the optical constants and resonance response of the metal.…”

Section: Theorymentioning

confidence: 99%

“…The conditions for SPR absorption are satisfied when (6) SPR only occurs for p-polarized incident light. 17,18 In reality SPR does not occur only at a single angle but over a relatively narrow range of angles determined by the optical constants and resonance response of the metal.…”

Section: Theorymentioning

confidence: 99%

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DNA hybridization using surface plasmon-coupled emission

Malicka

Gryczyński

et al. 2004

SPIE Proceedings

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We describe a new approach to measuring DNA hybridization using surface plasmon-coupled emission (SPCE). Excited fluorophores are known to couple with surface oscillations of electrons in thin metal films, typically 50 nm thick silver on a glass prism. These surface plasmons then radiate into the glass at a sharply defined angle determined by the emission wavelength and the optical properties of the glass and metal. This radiation has the same spectral profile as the emission spectrum of the fluorophores. We studied the emission due to Cy3-labeled DNA oligomers bound to complementary unlabeled oligomers which were themselves bound to the metal surface. Hybridization resulted in SPCE due to Cy3-DNA into the prism. Directional SPCE was observed whether the sample was illuminated from the sample side or through the glass substrate at the surface plasmon angle for the excitation wavelength. A large fraction of the total potential emission is coupled to the surface plasmons resulting in improved sensitivity. When illuminated through the prism at the surface plasmon angle, the sensitivity is increased due to the enhanced intensity of the resonance evanescent field. It is known that SPCE depends on proximity to the silver surface. As a result, changes in emission intensity are observed due to fluorophore localization even if hybridization does not affect the quantum yield of the fluorophore. The use of SPCE resulted in suppression of interfering emission from a noncomplementary Cy5-DNA oligomers due to weaker coupling of the more distant fluorophores with the surface plasmons. We expect SPCE to have numerous applications to nucleic acid analysis and for the measurement of bioaffinity reactions.Measurement of DNA hybridization is now a central component of biotechnology and medical diagnostics. A variety of approaches are available to detect DNA hybridization including the use of intercalating fluorophores, 1,2 dyes which displayed increased quantum yields upon binding to double stranded (ds) DNA, 3,4 fluorescence resonance energy transfer, 5,6 and excimer formation. 7 In all these methods hybridization is detected by a change in the emission spectral properties of the probe which occurs upon formation of double-stranded DNA, typically an increased quantum yield of the fluorophore or an increase in resonance energy transfer.We now describe a new approach to measurement of nucleic acid hybridization which does not depend on a spectral change in the fluorophores. In our approach the intensity change is due to localization of the fluorophore near a thin metal surface by the binding reaction. In several recent reports we described a phenomenon called surface plasmon-coupled emission (SPCE). [8][9][10] From theoretical studies it is known that this phenomenon occurs for excited fluorophores within about 200 nm of a thin continuous metallic surface, 11,12 in our case 50 nm thick silver on a glass substrate. A small number of experimental reports of SPCE have appeared, [13][14][15][16] the metal surface. The surface p...

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Section: Theorymentioning

confidence: 95%

“…17,18 In reality SPR does not occur only at a single angle but over a relatively narrow range of angles determined by the optical constants and resonance response of the metal.…”

Section: Theorymentioning

confidence: 99%

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DNA hybridization using surface plasmon-coupled emission

Malicka

Gryczyński

et al. 2004

SPIE Proceedings

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“…Over recent years SPR devices have been commercially exploited and widely applied to biosensing applications 3,5,6 taking advantage of the high surface sensitivity of the technique. Typically these applications use thin evaporated gold films coated with selective chemistries to detect the binding or reaction of target analytes from solution.…”

Section: Introductionmentioning

confidence: 99%

Waveguide surface plasmon resonance studies of surface reactions on gold electrodes

et al. 2002

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We describe the fabrication and characterisation of gold-coated graded index channel waveguide sensors designed for simultaneous electrochemical and surface plasmon resonance studies. The active sensing electrode area is a thin gold film between 0.5 and 5 mm in length and 200 mm wide deposited on top of a 3 mm wide waveguide which forms one arm of a Y-junction while the other arm of the Y-junction serves as a reference. Using these devices we have measured simultaneously the changes in transmittance through the device whilst carrying out cyclic voltammetry in either sulfuric or perchloric acid solution or during the deposition of an UPD layer of copper at the gold surface. In all cases we obtain stable and reproducible results which demonstrate the very high sensitivity of the devices to sub-monolayer changes occurring at the gold electrode surface. The response of these integrated optoelectrochemical devices is discussed in terms of a numerical model for the propagation of light within the waveguide structure.

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“…Esta característica de aumento de sensibilidade, importante para sistemas com rendimentos quânticos baixos, tem sido explorada na elaboração de técnicas analíticas e de sensores químicos por emissão de fluorescência [20,21].…”

Section: Palavras Chave: Nanopartículas De Prata Corantes Fluorescêunclassified

Modulação de fluorescência de amino coumarinas e acridinas por nanopartículas de prata

Sabatini¹

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A Deus pelo presente de viver.Ao Prof. Marcelo, pela orientação, confiança, paciência e ensinamentos.Aos amigos Manu, Ana, Robson pela amizade e por estarem sempre presentes.Aos amigos Suzana, Janildo, Sidney (Puff), Denis (MP), Josy, Bia, Marins, Glauco, Guilherme, Maira e Lívia.Ao Mauro, pelo auxílio e paciência.Ao pessoal da Seção de Informática, pelo auxílio e paciência.Ao Instituto de Química, pela estrutura e facilidades oferecidas.Ao CNPq, pela bolsa concedida.

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Peer Reviewed: SPR of Ultrathin Organic Films

Cited by 103 publications

References 30 publications

DNA hybridization using surface plasmon-coupled emission

DNA hybridization using surface plasmon-coupled emission

Waveguide surface plasmon resonance studies of surface reactions on gold electrodes

Modulação de fluorescência de amino coumarinas e acridinas por nanopartículas de prata

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