Peer Reviewed: HPLC: Past and Present.

Snyder, Lloyd R.

doi:10.1021/ac002846r

Cited by 36 publications

(18 citation statements)

References 16 publications

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“…significantly increased system pressures, drastic reductions in extra-column broadening, improved fittings, as well as faster and lower volume detectors. Interestingly these important developments, especially the use of smaller particles, shorter columns and higher pressures (up to 19,000 psi) precisely follow the historical trends in HPLC technology outlined by Snyder in his prescient article of 2000 1 although the “sub-two micron barrier” has now been breached. Indeed, many of these developments were anticipated in Giddings’s seminal work which compared the speed of separations in GC and LC 2 .…”

Section: Introductionmentioning

confidence: 77%

Perspectives on Recent Advances in the Speed of High-Performance Liquid Chromatography

2011

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Perhaps the most consistent trend in the development of HPLC since its inception in the 1960’s has been the continuing reach for ever faster analyses. The pioneering work of Knox, Horvath, Halasz, and Guiochon set forth a theoretical framework that was used early on to improve the speed of HPLC, primarily through the commercialization of smaller and smaller particles. Over the past decade, approaches to improving the speed of HPLC have become more diverse, and now practitioners of HPLC are faced with the difficult task of deciding which of these approaches will lead them to the fastest analysis for their application. Digesting the rich literature on the optimization of HPLC is a difficult task in itself, which is further complicated by contradictory marketing messages from competing commercial outlets for HPLC technology. In this perspectives article we provide an overview of the theoretical and practical aspects of the principal modern approaches to improving the speed of HPLC. We present a straightforward theoretical basis, informed by decades of literature on the problem of optimization, that is useful for comparing different technologies for improving the speed of HPLC. Through mindful optimization of conditions high performance separations on the sub-minute timescale are now possible and becoming increasingly common under both isocratic and gradient elution conditions, and the continued development of ultrafast separations will play an important role in the development of two-dimensional HPLC separations. Despite the relatively long history of HPLC as an analytical technique, there is no sign of a slow-down in the development of novel HPLC technologies.

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Section: Introductionmentioning

confidence: 77%

Perspectives on Recent Advances in the Speed of High-Performance Liquid Chromatography

2011

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“…(5) that an efficient way to reduce separation time is to use small particles as packing materials, since the separation time is proportional to the square of particle diameter. The evolution of HPLC has dictated the reduction of the particle size of column packings [34]. The size of the packing material has been reduced to current 1.5 -5 lm from greater than 100 lm employed in the 1960s.…”

Section: Separation Time Particle Size and Pressure Dropmentioning

confidence: 99%

Fundamental and practical aspects of ultrahigh pressure liquid chromatography for fast separations

Clausen

2007

J of Separation Science

161

115

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The ongoing development of HPLC has been focused on increasing the speed and efficiency of separations over the past decade. The advances in separation speed have been primarily related to the development of column technology and instrumentation. Relatively short columns packed with sub-2 microm particles provide high-speed separations while maintaining or increasing resolution. Ultrahigh pressure pump systems have been developed to overcome the high-pressure drop generated by such sub-2 microm packings. In this review, fundamental and practical aspects of ultrahigh pressure or ultrahigh performance liquid chromatography (U-HPLC) are discussed. Applications of fast U-HPLC separations are also presented.

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“…For a further background on the early days of HPLC, see [19,[31][32][33]. Additional historical details on the progress of HPLC after 1980 are provided by the collected biographies of several HPLC practitioners [34].…”

Section: A Short History Of Hplcmentioning

confidence: 99%

Introduction

2009

Introduction to Modern Liquid Chromatography

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Peer Reviewed: HPLC: Past and Present.

Cited by 36 publications

References 16 publications

Perspectives on Recent Advances in the Speed of High-Performance Liquid Chromatography

Perspectives on Recent Advances in the Speed of High-Performance Liquid Chromatography

Fundamental and practical aspects of ultrahigh pressure liquid chromatography for fast separations

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