Peer Reviewed: Analytical Bioluminescence and Chemiluminescence

Roda, Aldo; Guardigli, Massimo; Michelini, Elisa; Mirasoli, Mara; Pasini, Patrizia

doi:10.1021/ac031398v

Cited by 138 publications

(108 citation statements)

References 27 publications

(36 reference statements)

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“…In this technique, for example, the electrophoresis should be conducted with the usage of non-denatured gel and running at low temperature to keep the proteins active; otherwise CL signal cannot be seen. CL-imaging methods offer many advantages for high-throughput evaluation, high sensitivity, simple instrumentation, and exclusion of the usage of radioisotopes [8,9]. We reported a CL immunoassay for the sensitive detection of a CYP3A4 protein utilizing two antibodies and a dextran-luminol-biotin (DLB) polymer [10].…”

Section: Introductionmentioning

confidence: 99%

Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer

Zhang¹,

Shibata²,

Krawczyk³

et al. 2009

Talanta

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Facile and non-radioactive methods are desired for the sensitive detection and quantification of various proteins. Herein we describe a novel chemiluminescence (CL)-detection method of particular proteins based on direct binding of a dextran-luminol-biotin (DLB) CL polymer to the proteins on a poly(vinylidene difluoride) membrane. Among 32 kinds of the proteins screened, several proteins such as drug-metabolizing enzymes, cytochrome p450 (CYP)1A2, CYP2E1, and CYP3A4 had the ability to bind directly to the DLB polymer. The binding site in the polymer was owing to the framework of the modified dextran, which underwent oxidation and reduction procedures. This interaction might be the comprehensive effect of both electrostatic interaction and steric complementarities. CL intensity of the proteins detected by the polymer could be further enlarged by the mediation of avidin. The proposed CL-imaging method possesses potential as a rapid, facile, inexpensive and selective detection of the proteins.

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Section: Introductionmentioning

confidence: 99%

Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer

Zhang¹,

Shibata²,

Krawczyk³

et al. 2009

Talanta

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“…As it has been shown in numerous studies many drugs inhibit the bioluminescence of luminescent bacteria in a concentration-dependent manner, thus demonstrating its toxicity, antibacterial properties and other biological activities [6] [7]. It made bioluminescent inhibition test as one of the most common among others ones.…”

Section: Resultsmentioning

confidence: 99%

Determination of Binding Parameters of Miramistin to Serum Albumin by the Bioluminescent Method

Starodub¹,

Katsev²,

Abduramanova³

et al. 2014

ABC

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Bioassay based on the luminescent bacteria was used as a new method for the determination of the kinetic constants of ligand binding to the complexion agent. Miramistin as the cationic surface-active antiseptic and serum blood albumin was taken as a model of interacting agent's. It was concluded that this approach might have a wide range for applications, namely, in all cases where the ligand is an inhibitor of bacterial luminescence and its complex dose not effect the intensity of this process.

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“…Several thorough review papers have been published recently on analytical CL [69,70], its biotechnical and clinical applications [71,72], analytical ECL [73], and luminescence methods generally [74,75]. Also, CL detection for CE and LC was recently reviewed [76], along with ECL detection for microfluidic chips [77] and, more specifically, the current status of tris-(2,2'-bipyridyl) ruthenium (II) (Ru(bpy) 3 21 ) and its derivatives for ECL detection [78].…”

Section: Chemiluminescence and Electrochemiluminescence Detectionmentioning

confidence: 99%

Unconventional detection methods for microfluidic devices

2006

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The direction of modern analytical techniques is to push for lower detection limits, improved selectivity and sensitivity, faster analysis time, higher throughput, and more inexpensive analysis systems with ever-decreasing sample volumes. These very ambitious goals are exacerbated by the need to reduce the overall size of the device and the instrumentation -the quest for functional micrototal analysis systems epitomizes this. Microfluidic devices fabricated in glass, and more recently, in a variety of polymers, brings us a step closer to being able to achieve these stringent goals and to realize the economical fabrication of sophisticated instrumentation. However, this places a significant burden on the detection systems associated with microchip-based analysis systems. There is a need for a universal detector that can efficiently detect sample analytes in real time and with minimal sample manipulation steps, such as lengthy labeling protocols. This review highlights the advances in uncommon or less frequently used detection methods associated with microfluidic devices. As a result, the three most common methods -LIF, electrochemical, and mass spectrometric techniques -are omitted in order to focus on the more esoteric detection methods reported in the literature over the last 2 years.

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Peer Reviewed: Analytical Bioluminescence and Chemiluminescence

Cited by 138 publications

References 27 publications

Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer

Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer

Determination of Binding Parameters of Miramistin to Serum Albumin by the Bioluminescent Method

Unconventional detection methods for microfluidic devices

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