Peer Review #3 of "Expression patterns and the prognostic value of the EMILIN/Multimerin family members in low-grade glioma (v0.1)"

Abstract

The management of Low-grade glioma (LGG) remains a challenge because of the infiltrating nature of the tumor, which cannot be cured by surgical resection. The EMILIN/Multimerins containing the gC1q signature which were involved in many processes of tumor development. However, the expression of EMILIN/Multimerins in LGG and its prognostic value remains unclear. This study investigates the expression pattern, prognostic significance and function of EMILIN/Multimerins in LGG patients based on integrated bioinformatics analysis. The transcriptional levels and prognosis of EMILIN/Multimerins in LGG were analyzed by the ONCOMINE, GEPIA and UALCAN. The EMILIN/Multimerins' mutation, co-expression neighboring genes were analyzed via cBioProtal. TIMER and Metascape were used to reveal the potential mechanism of EMILIN/Multimerins in LGG. And we found that most EMILIN/Multimerins were overexpressed in LGG and share a clear association with immune cells. GEPIA confirmed that high levels of EMILIN/Multimerins were closely correlated with poor prognosis in LGG patients, except the MMRN2 in disease-free survival (DFS). The expression of EMILIN/Multimerins was related to different pathological grades. Potential mechanisms of different EMILIN/Multimerins in regulating LGG was revealed. Our findings showed that EMILIN/Multimerins might serve as novel prognostic biomarkers and possibly be a highpriority therapeutic target for LGG patients.