Peer Review #2 of "Histological evaluation of five suture materials in the telson ligament of the American horseshoe crab (Limulus polyphemus) (v0.1)"

Abstract

An ideal suture material supports healing, minimizes inflammation, and decreases the likelihood of secondary infection. While there are published recommendations for suture materials in some invertebrates, there are no published recommendations for Limulus polyphemus or any chelicerate. This study evaluates the histological reaction of horseshoe crabs to five commonly used suture materials: monofilament nylon, silk, poliglecaprone, polydioxanone, and polyglycolic acid. None of the materials were superior with regards to holding nor was there any dehiscence. Nylon evoked the least amount of tissue reaction. This work also provides a histopathological description of the soft membrane at the hinge area between the opisthosoma and telson (telson ligament) and comments on euthanasia with intracardiac eugenol.