Peer Review #1 of "Metamorphosis of the invasive ascidian Ciona savignyi: environmental variables and chemical exposure (v0.1)"
Abstract:In this study, the effects of environmental variables on larval metamorphosis of the solitary ascidian Ciona savignyi were investigated in a laboratory setting. The progression of metamorphic changes were tracked under various temperature, photoperiod, substrate, larval density, and vessel size regimes. Metamorphosis was maximised at 18°C, 12:12 h subdued light:dark, smooth polystyrene substrate, and 10 larvae mL-1 in a twelve-well tissue culture plate. Eliminating the air-water interface by filling culture ve… Show more
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