PEDV-spike-protein-expressing mRNA vaccine protects piglets against PEDV challenge

Zhao, Yongxiang; Fan, Baochao; Song, Xu; Gao, Jie; Guo, Rongli; Yi, Cheng; He, Zhaoming; Hu, Hongpeng; Jiang, Jianhao; Zhao, Lixiang; Zhong, Tianyi; Li, Bin

doi:10.1128/mbio.02958-23

Cited by 7 publications

(3 citation statements)

References 49 publications

(40 reference statements)

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“…A slightly more distant but mRNA vaccine-based veterinary example targeted porcine epidemic diarrhea virus (PEDV) [57]. PEDV is an enteric coronavirus of pigs and causes significant morbidity and mortality in piglets, representing significant losses in the pig industry [58].…”

Section: Discussionmentioning

confidence: 99%

Feline Infectious Peritonitis mRNA Vaccine Elicits Both Humoral and Cellular Immune Responses in Mice

Brostoff,

Savage,

Jackson

et al. 2024

Vaccines

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Feline infectious peritonitis (FIP) is a devastating and often fatal disease caused by feline coronavirus (FCoV). Currently, there is no widely used vaccine for FIP, and many attempts using a variety of platforms have been largely unsuccessful due to the disease’s highly complicated pathogenesis. One such complication is antibody-dependent enhancement (ADE) seen in FIP, which occurs when sub-neutralizing antibody responses to viral surface proteins paradoxically enhance disease. A novel vaccine strategy is presented here that can overcome the risk of ADE by instead using a lipid nanoparticle-encapsulated mRNA encoding the transcript for the internal structural nucleocapsid (N) FCoV protein. Both wild type and, by introduction of silent mutations, GC content-optimized mRNA vaccines targeting N were developed. mRNA durability in vitro was characterized by quantitative reverse-transcriptase PCR and protein expression by immunofluorescence assay for one week after transfection of cultured feline cells. Both mRNA durability and protein production in vitro were improved with the GC-optimized construct as compared to wild type. Immune responses were assayed by looking at N-specific humoral (by ELISA) and stimulated cytotoxic T cell (by flow cytometry) responses in a proof-of-concept mouse vaccination study. These data together demonstrate that an LNP–mRNA FIP vaccine targeting FCoV N is stable in vitro, capable of eliciting an immune response in mice, and provides justification for beginning safety and efficacy trials in cats.

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Section: Discussionmentioning

confidence: 99%

Feline Infectious Peritonitis mRNA Vaccine Elicits Both Humoral and Cellular Immune Responses in Mice

Brostoff,

Savage,

Jackson

et al. 2024

Vaccines

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“…For example, an mRNA lipid nanoparticle (mRNA-LNP) vaccine created by Li’s group, which contains the complete PEDV spike (S) protein, demonstrated the ability to stimulate strong PEDV-specific immune responses in piglets. This vaccine not only effectively shielded actively immunized piglets from PEDV infection but also imparted passive anti-PEDV immunity to neonatal piglets through the transfer of colostrum-derived antibodies from immunized sows [ 157 ]. mRNA vaccines have shown great efficacy in fighting novel coronavirus infections in humans.…”

Section: Types Of Vaccine Development Platforms and The Trialed Or Ge...mentioning

confidence: 99%

Towards a Safer Future: Enhancing Vaccine Development to Combat Animal Coronaviruses

Si,

Yu,

Dong

et al. 2024

Vaccines

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Coronaviruses (CoVs) are a large class of positively stranded RNA viruses that pose a significant threat to public health, livestock farming, and wild animals. These viruses have the ability to cross species barriers and cause devastating epidemics. Animals are considered to be intermediate hosts for many coronaviruses, and many animal coronaviruses also have the potential for cross-species transmission to humans. Therefore, controlling the epidemic transmission of animal coronaviruses is of great importance to human health. Vaccination programs have proven to be effective in controlling coronaviruses infections, offering a cost-effective approach to reducing morbidity and mortality, so the re-emergence of lethal coronaviruses emphasizes the urgent need for the development of effective vaccines. In this regard, we explore the progress in animal coronavirus vaccine development, covering the latest taxonomy of the main animal coronaviruses, spillover events, diverse vaccine development platforms, potential main targets for animal coronavirus vaccine development, and primary challenges facing animal coronavirus vaccines. We emphasize the urgent need to create a “dual-effect” vaccine capable of eliciting both cellular and humoral immune responses. The goal is to highlight the contributions of veterinary scientists in this field and emphasize the importance of interdisciplinary collaboration between the veterinary and medical communities. By promoting communication and cooperation, we can enhance the development of novel and super vaccines to combat human and animal coronavirus infections in the future.

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“…In pregnant sows, mRNA PED vaccination based on the receptor binding region (RBD) heterodimer generated neutralizing antibody levels comparable to those of the available inactivated vaccines [ 129 ]. Another PEDV vaccine that targets the spike (S) protein of PEDV induces a strong antibody response and antigen-specific T-cell responses in immunized piglets [ 130 ]. Furthermore, an mRNA-vectored Nipah virus (NiV) vaccine expressing soluble G glycoprotein candidates in pigs was developed using lipid nanoparticles.…”

Section: Nucleic-acid-based Vaccinesmentioning

confidence: 99%

A Comprehensive Review of Our Understanding and Challenges of Viral Vaccines against Swine Pathogens

Kamboj,

Dumka,

Saxena

et al. 2024

Viruses

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Pig farming has become a strategically significant and economically important industry across the globe. It is also a potentially vulnerable sector due to challenges posed by transboundary diseases in which viral infections are at the forefront. Among the porcine viral diseases, African swine fever, classical swine fever, foot and mouth disease, porcine reproductive and respiratory syndrome, pseudorabies, swine influenza, and transmissible gastroenteritis are some of the diseases that cause substantial economic losses in the pig industry. It is a well-established fact that vaccination is undoubtedly the most effective strategy to control viral infections in animals. From the period of Jenner and Pasteur to the recent new-generation technology era, the development of vaccines has contributed significantly to reducing the burden of viral infections on animals and humans. Inactivated and modified live viral vaccines provide partial protection against key pathogens. However, there is a need to improve these vaccines to address emerging infections more comprehensively and ensure their safety. The recent reports on new-generation vaccines against swine viruses like DNA, viral-vector-based replicon, chimeric, peptide, plant-made, virus-like particle, and nanoparticle-based vaccines are very encouraging. The current review gathers comprehensive information on the available vaccines and the future perspectives on porcine viral vaccines.

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PEDV-spike-protein-expressing mRNA vaccine protects piglets against PEDV challenge

Cited by 7 publications

References 49 publications

Feline Infectious Peritonitis mRNA Vaccine Elicits Both Humoral and Cellular Immune Responses in Mice

Feline Infectious Peritonitis mRNA Vaccine Elicits Both Humoral and Cellular Immune Responses in Mice

Towards a Safer Future: Enhancing Vaccine Development to Combat Animal Coronaviruses

A Comprehensive Review of Our Understanding and Challenges of Viral Vaccines against Swine Pathogens

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