Pediococcus pentosaceus IM96 Exerts Protective Effects against Enterohemorrhagic Escherichia coli O157:H7 Infection In Vivo

Li, Haixin; Xie, Xinqiang; Liu, Ying; Chen, Moutong; Xue, L.; Wang, Juan; Zhang, Jumei; Wu, Shi; Ye, Qinghua; Zhang, Shuhong; Yang, Rongrong; Zhao, Hui; Wu, Lei; Liang, Tingting; Ding, Yu; Wu, Qingping

doi:10.3390/foods10122945

Cited by 11 publications

(4 citation statements)

References 46 publications

(49 reference statements)

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“…It has been reported that Pediococcus pentosaceus CECT 8330 protects DSS‐induced colitis and increased the fecal SCFAs level in mice (Dong et al., 2022). P. pentosaceus IM96 alleviated intestinal mucosal damage and increased the level of SCFAs in mice infected by EHEC O157:H7 (Li et al., 2021). Pediococcus acidilactici FZU106 intervention significantly increased the SCFAs levels in high‐fat diet‐fed rats, alleviating lipid metabolism disorder (Zhang et al., 2022).…”

Section: Resultsmentioning

confidence: 99%

Synthesis of queuine by colonic gut microbiome via cross‐feeding

Yan,

Xiang,

Shi

et al. 2023

Food Frontiers

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Queuine (Que) is an essential micronutrient, and its deficiency will lead to errors in tRNA modification and protein misfold, further to diseases. Meanwhile, the content of Que in food will be further reduced due to the modern planting pattern and processing. In this study, an idea, synthetic biology of gut microbiome, was introduced to meet people's demand for Que. Further, various food can be used as a media for promoting the growth of synthetic biology of flora and the concentrations of Que. Through this research, gut bacteria (Escherichia coli K12, Bacteroides fragilis ATCC25285, and Lactobacillus reuteri DSM20058) with abilities of Que independent synthesis in vitro were selected, and higher productivity of Que was fulfilled through the above three strains cocultivation via cross‐feeding. Moreover, the effect of Que on the structure of microbiome was also studied, in order to figure out the continuous synthesis of Que by this coculture system in colonic situation. Que could enhance bacterial α‐diversity, increase the abundance of Limosilactobacillus, Pediococcus, and Lactobacillus, while decrease the abundance of Eschi‐Shigella. Three bacteria combination intervention changed intestinal microbial composition, and distribution of enzymes transglycozyme, QueA, and QueG that involved in last three steps of Que synthesis in microorganisms was significantly changed. These results provide an experimental basis for colonic synthesis of Que via food intervention to solve the deficiency Que in human body. Here, we regard gut microbes as a fermentation system in human body, providing a constant supply of tiny but important substance that humans need.

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Section: Resultsmentioning

confidence: 99%

Synthesis of queuine by colonic gut microbiome via cross‐feeding

Yan,

Xiang,

Shi

et al. 2023

Food Frontiers

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“…The agar diffusion assay was used to identify the bacteriostatic ability of cell-free supernatant (CFS) by Zhang and Li et al [ 27 , 28 ]. A 15 mL aliquot of 0.4% soft agar with 2% bacterial culture medium was poured into a sterile Petri dish, and sterile Oxford cups (ϕ = 7.64 mm) were put on the surface of the medium.…”

Section: Methodsmentioning

confidence: 99%

Dissecting of the AI-2/LuxS Mediated Growth Characteristics and Bacteriostatic Ability of Lactiplantibacillus plantarum SS-128 by Integration of Transcriptomics and Metabolomics

Qian

et al. 2022

Foods

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Lactiplantibacillus plantarum could regulate certain physiological functions through the AI-2/LuxS-mediated quorum sensing (QS) system. To explore the regulation mechanism on the growth characteristics and bacteriostatic ability of L. plantarum SS-128, a luxS mutant was constructed by a two-step homologous recombination. Compared with ΔluxS/SS-128, the metabolites of SS-128 had stronger bacteriostatic ability. The combined analysis of transcriptomics and metabolomics data showed that SS-128 exhibited higher pyruvate metabolic efficiency and energy input, followed by higher LDH level and metabolite overflow compared to ΔluxS/SS-128, resulting in stronger bacteriostatic ability. The absence of luxS induces a regulatory pathway that burdens the cysteine cycle by quantitatively drawing off central metabolic intermediaries. To accommodate this mutations, ΔluxS/SS-128 exhibited lower metabolite overflow and abnormal proliferation. These results demonstrate that the growth characteristic and metabolism of L. plantarum SS-128 are mediated by the AI-2/LuxS QS system, which is a positive regulator involved in food safety. It would be helpful to investigate more bio-preservation control potential of L. plantarum, especially when applied in food industrial biotechnology.

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“…Blood was collected by heart puncture after fasting for about 16 h in order to conduct the serum biochemical analysis. The concentrations of biological indexes related to hypertension were determined by the commercial enzyme linked immunosorbent assay (ELISA) kits from Beijing Dongge Biotechnology Co., Ltd. (Beijing, China) following the manufacturer’s protocols [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Antihypertensive Activity of Milk Fermented by Lactiplantibacillus plantarum SR37-3 and SR61-2 in L-NAME-Induced Hypertensive Rats

Yuan

Chen

et al. 2022

Foods

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Probiotic fermented milk can lower the incidence rate of hypertension and is beneficial to the regulation of the intestinal microecology. However, the underlying molecular mechanism remains elusive. Here, we evaluated the role of the gut microbiota and its metabolites in the antihypertensive effect of milk fermented by the Lactiplantibacillus plantarum strains SR37-3 (PFM-SR37-3) and SR61-2 (PFM-SR61-2) in Ng-nitro-L-arginine methyl ester induced hypertensive rats. The results showed that PFM-SR37-3 and PFM-SR61-2 intervention significantly lowered the blood pressure (BP) of NG-nitro-L-arginine methyl ester induced hypertensive rats and attenuated renal injury. In particular, long-term administration of PFM inhibited a progressive elevation in SBP (170.22 ± 8.40 and 133.28 ± 6.09 by model group and PFM-SR37-3 treated model group, respectively, at the end of the 4 weeks; p < 0.01 PFM-SR37-3 treated model group versus model group) and DBP (133.83 ± 5.91 and 103.00 ± 6.41 by model group and PFM-SR37-3 treated model group, respectively, at the end of the 4 weeks; p < 0.01 PFM-SR37-3 treated model group versus model group). PFM-SR37-3 and PFM-SR61-2 reshaped the gut microbiome and metabolome, and especially regulated the metabolic levels of L-phenylalanine, L-methionine and L-valine in the intestine and blood circulation. The analysis of the target organ’s aortic transcriptome indicated that the protective effects of PFM-SR37-3 and PFM-SR61-2 were accompanied by the modulation of the BP circadian rhythm pathway, which was conducive to cardiovascular function. Vascular transcriptomic analysis showed that circadian rhythm and AMPK might be potential targets of hypertension. In addition, the ACE inhibition rates of Lactiplantibacillus plantarum SR37-3 and Lactiplantibacillus plantarum SR61-2 in vitro were 70.5% and 68.9%, respectively. Our research provides new insights into novel and safe options for hypertension treatment.

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Pediococcus pentosaceus IM96 Exerts Protective Effects against Enterohemorrhagic Escherichia coli O157:H7 Infection In Vivo

Cited by 11 publications

References 46 publications

Synthesis of queuine by colonic gut microbiome via cross‐feeding

Synthesis of queuine by colonic gut microbiome via cross‐feeding

Dissecting of the AI-2/LuxS Mediated Growth Characteristics and Bacteriostatic Ability of Lactiplantibacillus plantarum SS-128 by Integration of Transcriptomics and Metabolomics

Antihypertensive Activity of Milk Fermented by Lactiplantibacillus plantarum SR37-3 and SR61-2 in L-NAME-Induced Hypertensive Rats

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