Pediococcus lolii sp. nov., isolated from ryegrass silage

Doi, Kunio; Nishizaki, Yousuke; Fujino, Yasuhiro; Ohshima, Toshihisa; Ohmomo, Sadahiro; Ogata, Seiya

doi:10.1099/ijs.0.005793-0

Cited by 25 publications

(23 citation statements)

References 13 publications

(8 reference statements)

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“…These include Lactobacillus taiwanensis (Wang et al 2009) and Pediococcus lolii (Doi et al 2009). analysed laboratory silages made with Italian ryegrass, maize, guinea grass and rhodes grass ensiled with and without L. plantarum or Lactobacillus brevis inoculants.…”

Section: New Microbial Species In Silagementioning

confidence: 99%

Recent advances in silage microbiology

Muck

2013

AFSci

171

123

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Recent advances in silage microbiology are reviewed. Most new techniques in silage microbiology use the polymerase chain reaction (PCR) to make copies of a portion of the DNA in microorganisms. These techniques allow us to identify and quantify species as well as do community analysis. The PCR-based techniques are uncovering new species, both bacteria and fungi, during storage and feeding. Silage inoculants are widely available, but of greater interest has been research investigating why inoculants are so successful. Various inoculant strains have been found to produce bacteriocins and other compounds that inhibit other bacteria and fungi, improving their chances for success. In vitro ruminal fermentation research is showing that some inoculated silages affect rumen microorganisms, reducing methane in some cases and increasing microbial biomass production in others. Better understanding of silage microbiology will allow us to better manage silos and develop better inoculants to improve silage quality.

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Section: New Microbial Species In Silagementioning

confidence: 99%

Recent advances in silage microbiology

Muck

2013

AFSci

171

123

View full text Add to dashboard Cite

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“…The species description was consulted when these sources were inconsistent (24)(25)(26)(27)(28)(29)(30). The assignment of the fermentation type of pediococci was based on the work of Franz et al (21) and the description of the species (31)(32)(33)(34)(35).…”

Section: Methodsmentioning

confidence: 99%

A Genomic View of Lactobacilli and Pediococci Demonstrates that Phylogeny Matches Ecology and Physiology

Zheng

Ruan

Sun

et al. 2015

Appl Environ Microbiol

211

210

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cLactobacilli are used widely in food, feed, and health applications. The taxonomy of the genus Lactobacillus, however, is confounded by the apparent lack of physiological markers for phylogenetic groups of lactobacilli and the unclear relationships between the diverse phylogenetic groups. This study used the core and pan-genomes of 174 type strains of Lactobacillus and Pediococcus to establish phylogenetic relationships and to identify metabolic properties differentiating phylogenetic groups. The core genome phylogenetic tree separated homofermentative lactobacilli and pediococci from heterofermentative lactobacilli. Aldolase and phosphofructokinase were generally present in homofermentative but not in heterofermentative lactobacilli; a twodomain alcohol dehydrogenase and mannitol dehydrogenase were present in most heterofermentative lactobacilli but absent in most homofermentative organisms. Other genes were predominantly present in homofermentative lactobacilli (pyruvate formate lyase) or heterofermentative lactobacilli (lactaldehyde dehydrogenase and glycerol dehydratase). Cluster analysis of the phylogenomic tree and the average nucleotide identity grouped the genus Lactobacillus sensu lato into 24 phylogenetic groups, including pediococci, with stable intra-and intergroup relationships. Individual groups may be differentiated by characteristic metabolic properties. The link between phylogeny and physiology that is proposed in this study facilitates future studies on the ecology, physiology, and industrial applications of lactobacilli. Lactobacilli are significant members of animal and human microbiota and of the plant phyllosphere. Owing to their stable association with humans as well as raw material for food production, lactobacilli also occur in many or most food fermentations. Lactobacilli are at the interface of aerobic and anaerobic life. Many lactobacilli retain the conditional capacity for respiration (1), but their ecology and physiology are mainly related to the fermentative conversion of sugars to organic acids (2, 3). Lactobacilli employ the Embden-Meyerhof pathway (glycolysis) and/or the phosphoketolase pathway for conversion of hexoses (2). These pathways have a low energetic efficiency; lactobacilli compensate for this disadvantage by rapid depletion of carbon sources and by accumulation of organic acids to inhibit competitors. The evolution and ecology of lactobacilli are shaped by niche adaptation and reduction of genome size (4). Many species, e.g., Lactobacillus plantarum, maintain a genetic diversity that enables occupation of diverse ecological niches (5). Other species are highly specialized and reduce their genomes more extensively. Examples include the insect-associated Lactobacillus fructivorans (6); Lactobacillus iners, a species specialized for the female human urogenital tract (7); and Lactobacillus reuteri, a host-specific intestinal symbiont of vertebrate animals (8). Lactobacillus delbrueckii has undergone a very recent reduction of genome size to adapt to dairy fermentations ...

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“…T , for which they proposed the name Pediococcus lolii (Doi et al, 2009). The description was based on a single strain isolated from ryegrass silage that was deposited in the DSMZ and JCM culture collections as DSM 19927 T and JCM 15055 T , respectively.…”

mentioning

confidence: 99%

Pediococcus lolii DSM 19927T and JCM 15055T are strains of Pediococcus acidilactici

Wieme

Cleenwerck

Landschoot

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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Strain NGRI 0510QT , isolated from ryegrass silage, was recently classified as a representative of a novel Pediococcus species, Pediococcus lolii Doi et al. 2009. It was deposited in the DSMZ and JCM culture collections as DSM 19927 T and JCM 15055 T , respectively. A polyphasic taxonomic study, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, pheS and 16S rRNA gene sequence analysis, fluorescent amplified fragment length polymorphism and DNA-DNA hybridization, was used to prove that both subcultures of the type, and only, strain of this species are strains of Pediococcus acidilactici.In 2009, Doi and colleagues reported on a novel strain belonging to the genus Pediococcus, NGRI 0510QT , for which they proposed the name Pediococcus lolii (Doi et al., 2009). The description was based on a single strain isolated from ryegrass silage that was deposited in the DSMZ and JCM culture collections as DSM 19927 T and JCM 15055 T , respectively. Their study revealed that the strain exhibited distinct phenotypic characteristics, divergent sequences of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region, and low rates of DNA-DNA hybridization in comparison with the type strains of Pediococcus acidilactici DSM 20284 T (5LMG 11384 T ) and Pediococcus pentosaceus DSM 20336 T (5LMG 11488 T ).The present study was initiated upon analysis of the P. lolii type strain accessioned from the JCM culture collection, JCM 15055 T (5LMG 25667 T ), by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which failed to discriminate P. lolii LMG 25667T from P. acidilactici strains. Subsequently, DSM 19927 T (5LMG 27029 T ) was accessioned from the DSMZ culture collection and the polyphasic taxonomic study described below was performed.All strains were grown on MRS agar (Oxoid) at 28 uC in anaerobic atmosphere, except strain P. acidilactici LMG 11384T , which was cultured in an aerobic atmosphere. Prior to MALDI-TOF MS analysis, strains were subcultured twice. Five to ten milligrams of wet cells were suspended in MilliQ-water comprising 75 % pure ethanol. Subsequently, formic acid and acetonitrile were added in a 1 : 1 (v/v) ratio to the bacterial cell pellet. After shaking vigorously, 1 ml supernatant (5the cell extract) was spotted onto a MALDI-TOF MS stainless steel target plate. Spots were overlaid with 1 ml matrix, which consisted of 5 mg acyano-4-hydroxycinnamic acid dissolved in 1 ml acetonitrile/trifluoroacetic acid/MilliQ-solvent (50 : 2 : 48). Prior to analysis, the mass spectrometer was externally calibrated using a peptide mixture of adrenocorticotropic hormone (fragment 18-39) (Sigma-Aldrich), insulin (Sigma-Aldrich), ubiquitin (Sigma-Aldrich), cytochrome C (Sigma-Aldrich) and myoglobin (Sigma-Aldrich). A 4800 Plus MALDI TOF/TOF Analyser (Applied Biosystems) was used in linear mode and covered a mass range of 2-20 kDa. The mass spectrometer used a 200 Hz frequency tripled Nd : YAG laser, operating at a wavelength of 355 nm. Generated ...

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Pediococcus lolii sp. nov., isolated from ryegrass silage

Cited by 25 publications

References 13 publications

Recent advances in silage microbiology

Recent advances in silage microbiology

A Genomic View of Lactobacilli and Pediococci Demonstrates that Phylogeny Matches Ecology and Physiology

Pediococcus lolii DSM 19927T and JCM 15055T are strains of Pediococcus acidilactici

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