Pediocin SA-1, an antimicrobial peptide from Pediococcus acidilactici NRRL B5627: Production conditions, purification and characterization

Anastasiadou, Sofia; Papagianni, Maria; Filiousis, George; Ambrosiadis, Ioannis; Koidis, P.

doi:10.1016/j.biortech.2007.11.015

Cited by 101 publications

(77 citation statements)

References 37 publications

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“…and found to be very effective against the anaerobic Clostridium sporogenes and Clostridium thiaminolyticum (Papagianni and Anastasiadou, 2009). This bacteriocin was heat stable for up to 60 min at 121°C, not impaired even following incubation at 30°C for 1 week at pH values ranging between 3.0 and 12.0, and found to be resistant to treatment with trypsin, α-chymotrypsin, pepsin, and papain, but not to proteinase K (Anastasiadou et al, 2008).…”

Section: Reduction Of Histamine Accumulation By the Bacteriocin Of Labmentioning

confidence: 99%

Inhibitory effect of bacteriocin-producing lactic acid bacteria against histamine-forming bacteria isolated from Myeolchi-jeot

Lim

2016

Fish Aquatic Sci

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The objectives of this study were to identify the histamine-forming bacteria and bacteriocin-producing lactic acid bacteria (LAB) isolated from Myeolchi-jeot according to sequence analysis of the 16S rRNA gene, to evaluate the inhibitory effects of the bacteriocin on the growth and histamine accumulation of histamine-forming bacteria, and to assess the physico-chemical properties of the bacteriocin. Based on 16S rRNA gene sequences, histamine-forming bacteria were identified as Bacillus licheniformis MCH01, Serratia marcescens MCH02, Staphylococcus xylosus MCH03, Aeromonas hydrophila MCH04, and Morganella morganii MCH05. The five LAB strains identified as Pediococcus acidilactici MCL11, Leuconostoc mesenteroides MCL12, Enterococcus faecium MCL13, Lactobacillus sakei MCL14, and Lactobacillus acidophilus MCL15 were found to produce an antibacterial compound with inhibitory activity against the tested histamine-producing bacteria. The inhibitory activity of these bacteriocins obtained from the five LAB remained stable after incubation at pH 4.0-8.0 and heating for 10 min at 80°C; however, the bacteriocin activity was destroyed after treatment with papain, pepsin, proteinase K, α-chymotrypsin, or trypsin. Meanwhile, these bacteriocins produced by the tested LAB strains also exhibited histamine-degradation ability. Therefore, these antimicrobial substances may play a role in inhibiting histamine formation in the fermented fish products and preventing seafood-related food-borne disease caused by bacterially generated histamine.

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Section: Reduction Of Histamine Accumulation By the Bacteriocin Of Labmentioning

confidence: 99%

Inhibitory effect of bacteriocin-producing lactic acid bacteria against histamine-forming bacteria isolated from Myeolchi-jeot

Lim

2016

Fish Aquatic Sci

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“…Moreover, BLSs from M3, M4 and M6 were stable after autoclaving at 121 C for 15 min. Other bacteriocins, such as Pediocin SA-1 (Anastasiadou et al, 2008) and enterocin Se-K4 (Eguchi et al, 2001), are also heat-stable bacteriocins. This means that the obtained supernatants can be used under fi eld conditions of different temperatures.…”

Section: Discussionmentioning

confidence: 99%

Isolation of bacteriocin-like substances producing bacteria from finished cattle-manure compost and activity evaluation against some food-borne pathogenic and spoilage bacteria

Abdel-Mohsein

Yamamoto

Otawa

et al. 2010

J. Gen. Appl. Microbiol.

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IntroductionComposting is one of the most natural processes capable of stabilizing organic wastes. Proper composting effectively destroys pathogens and weed seeds through the high temperature achieved by the metabolic heat generated by microorganisms. Because animal waste possibly contains viral, bacterial, and protozoan zoonotic pathogens, the application of untreated livestock waste could be a hygienic risk for humans (Hanajima et al., 2006). Application of cow manure or composted manure in agricultural practice could potentially cause contamination of foodstuffs with pathogenic bacteria such as E. coli O157:H7, Listeria monocytogenes, Clostridia sp., Mycobacterium sp., and Yersinia enterocolitica. The pathogens in animal manure are placed in an un-J. Gen. Appl. Microbiol., 56, 151 161 (2010) A fi nished compost sample was examined for bacteriocin-like substance production against fi ve pathogenic bacteria: Salmonella typhimurium EF 85-9, Escherichia coli O157:H7 ATCC 43888, Enterococcus faecalis JCM 8726, Staphylococcus aureus JCM 2151, and Yersinia enterocolitica JCM 7577. At the preliminary detection of bacterial stains exhibiting antimicrobial activity from the compost sample, thirteen strains could be isolated. Screening of the inhibitory activity was done using agar-well diffusion assay and Microtiter plate growth assay. Six bacterial strains from the compost showed an antimicrobial activity against one or more of the tested indicator strains. Four strains (M1 M4) belonged to Shigella species and the other two strains (M5 and M6) belonged to Salmonella species. The antimicrobial activity was sensitive for α-chymotrypsin and papain. The antimicrobial substances from M3, M4 and M6 were heat stable when heated for 15 min at 121 C with 100% relative activity. The bacteriocin-like substance produced by strain M2 was partially characterized. It exhibited an inhibitory activity against the tested food-borne pathogenic and spoilage bacteria, except Enterobacter aerogenes JCM 1235 and Lactobacillus plantarum subsp. plantarum JCM 1149. It was stable at a wide range of pH (3 11). There was no loss of activity for up to 3 weeks when stored at 4 and 20 C or for up to 2 weeks when stored at 28 and 80 C. This is the fi rst report indicating the presence of bacteriocin-like activity in animal manure compost.

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“…1). This decrease was probably due to the effect of extracellular proteases, adsorption to cell surfaces, and to feedback regulation (ANASTASIADOU et al, 2008). …”

Section: Identifi Cation Of Bacteriocin-producing Lab Strain and Bactmentioning

confidence: 98%

Antilisterial active compound from lactic acid bacteria present on fresh iceberg lettuce

Ramos¹,

Ferreira²,

Brandão³

et al. 2016

Acta Alimentaria

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Pediococcus pentosaceus DT016, a bacteriocin producing strain, was isolated from fresh lettuce. A protein with antilisterial activity (bacDT016), between 11 to 17 kDa, was identifi ed and characterized as the bioactive substance from the LAB culture. Highest bacteriocin production was noted after 15 h of growth. Antibacterial activity was affected by some enzymes and detergents, as well as by temperatures equal or above 80 ºC. DT016 strain contains an 1110 bp DNA fragment with homology to pediocin AcH/PA-1.

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Pediocin SA-1, an antimicrobial peptide from Pediococcus acidilactici NRRL B5627: Production conditions, purification and characterization

Cited by 101 publications

References 37 publications

Inhibitory effect of bacteriocin-producing lactic acid bacteria against histamine-forming bacteria isolated from Myeolchi-jeot

Inhibitory effect of bacteriocin-producing lactic acid bacteria against histamine-forming bacteria isolated from Myeolchi-jeot

Isolation of bacteriocin-like substances producing bacteria from finished cattle-manure compost and activity evaluation against some food-borne pathogenic and spoilage bacteria

Antilisterial active compound from lactic acid bacteria present on fresh iceberg lettuce

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