Pediocin A, a bacteriocin produced by Pediococcus pentosaceus FBB61

Piva, Andrea; Headon, Denis R.

doi:10.1099/00221287-140-4-697

Cited by 77 publications

(55 citation statements)

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“…However, the reactivity for the P. acidilactici P20 strain was much lower than for the other P. acidilactici strains. A much lower reactivity, negligible reactivity or no reactivity was observed with the supernatants of Pediococcus pentosaceus FBB61, a pediocin A producer (Piva & Headon, 1994), E. faecium T136, an enterocin A and B producer , E. faecium P13, an enterocin P producer (Cintas et al, 1998), Lb. sakei LTH673, a sakacin P producer (Tichaczek et al, 1994), Lb.…”

Section: Immunoreactivity Of the Rabbit Antipeptide Antibodies To Difmentioning

confidence: 99%

Antibodies to a synthetic 1–9-N-terminal amino acid fragment of mature pediocin PA-1: sensitivity and specificity for pediocin PA-1 and cross-reactivity against Class IIa bacteriocins

Martínez

Herranz

et al. 1999

Microbiology

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Polyclonal antibodies specific for pediocin PA-1 (PedA1) were generated by immunization of rabbits with a chemically synthesized 1-9-N-terminal amino acid fragment of this bacteriocin (PH1) conjugated to the carrier protein keyhole limpet haemocyanin (KLH). The PH1 fragment holds a highly conserved amino acid sequence with closely related Class IIa bacteriocins. The sensitivity and specificity of the PH1-KLH-generated rabbit polyclonal antibodies were evaluated by the development of various ELISAs, such as a non-competitive indirect ELISA (NCI-ELISA), a competitive indirect ELISA (CI-ELISA), a competitive direct ELISA (CD-ELISA) and a sandwich ELISA (S-ELISA), and by protein slot-blotting and Western blotting. NCI-and CI-ELISA were valuable for detecting the existence of PedA1-specific antibodies in the sera of immunized rabbits. The limit of detection of PedA1 in MRS medium was found to be 05 µg ml N1 in NCI-ELISA, while CI-ELISA on plates coated with purified PedA1 increased the affinity of the PH1-KLH-generated antibodies for PedA1 ; the limit of detection of PedA1 was less than 001 µg ml N1 and 50 % binding inhibition was achieved with 01 µg PedA1 ml N1 . Similarly, the limits of detection of PedA1 in MRS medium were found to be 5 µg ml N1 by protein slotblotting and 001 µg ml N1 by Western blotting. Most importantly, PH1-KLHgenerated polyclonal antibodies detected the presence of PedA1 in the supernatants of the producing strains of Pediococcus acidilactici 347, Z102, A172, X13 and P20, with no reactivity or negligible immunoreactivity with the supernatants of other lactic acid bacteria producing or not producing closely related or different bacteriocins. The approaches taken for the selection of the bacteriocin peptide fragment, the generation of antibodies and the development of immunoassays could prove useful for the generation and evaluation of antibodies of adequate specificity for other bacteriocins of interest in the food industry.

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Section: Immunoreactivity Of the Rabbit Antipeptide Antibodies To Difmentioning

confidence: 99%

Antibodies to a synthetic 1–9-N-terminal amino acid fragment of mature pediocin PA-1: sensitivity and specificity for pediocin PA-1 and cross-reactivity against Class IIa bacteriocins

Martínez

Herranz

et al. 1999

Microbiology

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“…and Clostridia (Piva and Headon, 1994) , but it had no direct effect against any gram-negative bacteria tested.…”

Section: -mentioning

confidence: 99%

“…Interestingly, Clostridia are one of the most gas-producing group of bacteria in the intestine (Hartemink and Rombouts, 1997) and they are considered to be sensitive to pediocin A. Pediocin A modified tryptophan degradation by caecal microflora, causing a constant reduction of skatole concentration by 21.5% (P<0.05) between 16 and 48 h. Pediocin A also caused an unexpected increase (53%) in indole concentration between 4 and 48 h (P<0.05; Figure 3), but no other effect could be observed on any indolic compound analyzed. Interestingly, many strains of intestinal bacteria can produce indole from L-tryptophan, whereas only some strains of the Clostridium and Lactobacillus genera are reported to produce skatole (Jensen et al, 1995) and show sensitivity to pediocin A (Piva and Headon, 1994).…”

Section: -mentioning

confidence: 99%

“…Since the mode of action of some probiotics can be related to the production of antimicrobial substances, we investigated the effect of pediocin A (a bacteriocin produced by Pediococcus pentosaceus FBB61; Piva and Headon, 1994) in in vitro caecal fermentation (Piva et al, 1995).The antimicrobial spectrum of this bacteriocin includes several strains of Listeria monocytogenes (Spelhaug and Harlander, 1989), Lactobacillus and Staphylococcus spp. and Clostridia (Piva and Headon, 1994) , but it had no direct effect against any gram-negative bacteria tested.…”

Section: -mentioning

confidence: 99%

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Non-conventional feed additives

Piva¹

1998

J. Anim. Feed Sci.

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It is important that alternatives to antimicrobials for animal growth promotion are found, raising the need for a deeper understanding of the microflora physiology and interaction with the host. In this perspective, non-conventional feed additives do not have to dramatically alter the activity of the intestinal microflora, but rather have to modulate the ecosystem in which potentially toxic compounds are produced, and improve the balance of beneficial bacteria showing interesting and welldefined phenotypes. Dietary characteristics, environmental hygiene, and the risk of inducing a resistant response should all be carefully considered when designing a long-term strategy. The roles of organic acids, non-digestible oligosaccharides and natural antimicrobials are discussed.

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“…pentosaceus ATCC 43200, also known as FBB61, was isolated in 1953 from cucumber fermentation and produced pediocin A (Fleming, Etchells, Costilow, 1975), which was shown to belong to the class III bacteriocins (Klaenhammer, 1993), with molecular weight of 80 kDa and a broad range of activity against Gram-positive bacteria (Piva, Headon, 1994).…”

Section: Introductionmentioning

confidence: 99%

Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms

Azevedo

Molinari

Oliveira

2018

Braz. J. Pharm. Sci.

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Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria, which exhibit a large spectrum of action against spoilage bacteria and foodborne pathogens. Successful application of techniques for quantitative or qualitative bacteriocin determination relies not only on the sensitivity of the test-microorganisms, but also on the agar-medium employed. Cell free supernatants are routinely used to preliminary screen for antimicrobial activity of bacteria by means of the agar well diffusion method, but the supernatant may also include other molecules (such as medium components and/or intracellular compounds) accidentally released during cell free supernatant preparation, which may interfere with the assay. Reproducibility of bacteriocin activity against the same test-microorganisms is an important factor to be considered. Unfortunately, no specific information about bioassays standardization to determine bacteriocin activity is available in the literature. In this work, growth inhibition by means of the agar well diffusion assays were carried out on different agar-media showing a strong dependence on the agar-medium used, indicating that the inhibitory effects could also depend on the diffusion of exudates that are included in the cell-free supernatant. The results presented in this communication show that selection of the agar-medium is crucial for the bioassay response.

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Pediocin A, a bacteriocin produced by Pediococcus pentosaceus FBB61

Cited by 77 publications

References 14 publications

Antibodies to a synthetic 1–9-N-terminal amino acid fragment of mature pediocin PA-1: sensitivity and specificity for pediocin PA-1 and cross-reactivity against Class IIa bacteriocins

Antibodies to a synthetic 1–9-N-terminal amino acid fragment of mature pediocin PA-1: sensitivity and specificity for pediocin PA-1 and cross-reactivity against Class IIa bacteriocins

Non-conventional feed additives

Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms

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