Pedigree and Molecular Analyses in the Assessment of Genetic Variability of the Polish Greyhound

Abstract

The aim of the study was to assess the genetic variability of the Polish Greyhound population based on pedigree analysis and molecular DNA testing and to determine the degree of relatedness among individuals in the population. Pedigree data of 912 Polish Greyhounds recorded in pedigree books since they were opened for this breed were analyzed. For molecular testing, DNA was obtained from cheek swabs taken from 235 dogs of the tested breed. A panel of 21 markers (Short Tandem Repeat—STR) was used. The mean inbreeding determined for the Polish Greyhound population based on pedigree analyses was low and amounted to 11.8%, but as many as 872 individuals of the 912 dogs in the studied population were inbred. A total of 83 founders (at least one unknown parent) were identified, among which 27 founders had both unknown parents. Full-sibling groups consisted of 130 individuals, with a minimum and maximum litter size of 2 and 16, respectively. The average litter size was 5.969. Gene diversity calculated based on the mean kinship matrix was 0.862 and the population mean kinship was 0.138. The founder genome equivalent based on the mean kinship matrix was 3.61; the founder genome surviving level was 12.34; the mean Ne was estimated at 21.76; and the Ne/N ratio was 0.135. The FIS inbreeding coefficient for 21 STR was negative, and the mean FIS value for all loci had a low negative value (−0.018). These values suggest a low level of inbreeding in the examined breed as well as the avoidance of mating related animals.

Kazakh national dog breed Tazy: What do we know?

“…The average polymorphism value based on all Tazy dogs was 0.739, a value very similar to that obtained for Greyhounds from South Korea (0.73) [13]. In contrast, the PIC value in a Polish Greyhound population was much lower (0.597), as reported by Goleman et al [31]. The highest level of polymorphism was observed at the REN169D01 and AHT121 loci, where the PIC value exceeded 0.850.…”

Genetic Diversity and Trends of Ancestral and New Inbreeding in Deutsch Drahthaar Assessed by Pedigree Data

“…Czech Spotted Dogs (F = 0.36), Nova Scotia Duck Tolling Retrievers (F = 0.26), American dog guides (F = 0.15/0.26) and Polish Lowland Sheepdogs (F = 0.18) had particularly high inbreeding coefficients [ 15 , 16 , 17 , 18 ]. Other highly inbred dog breeds included Polish Hunting Dogs and Greyhounds, Lancashire Heeler, Ibizan hounds, Bichon frise, Lundehund and Hungarian Border Collies [ 13 , 16 , 19 , 20 , 21 , 22 ]. To our knowledge, no pedigree analysis of genetic diversity and inbreeding measures was conducted for DD dogs.…”