Pedagogy

Ryndak, Valentina G.

doi:10.12737/25026

Cited by 2 publications

(6 citation statements)

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“…IrtA/IrtB is a heterdimeric protein involved in both the import of ferric-carboxymycobactin into the Mtb cytoplasm and the reduction of iron in the ferric-carboxymycobactin complex to release iron into the cytoplasm. 97,98 3.6.2 Potential protein players in the periplasm-The current consensus is that hydrophobic mycobactins are cell-surface receptors or cell-wall associated and are involved in transporting ferric iron from the extracellular environment through the cell-wall environment. In support of this hypothesis, experiments show that ferric-carboxymycobactin can transfer its ferric iron cargo to mycobactin.…”

Section: 6mentioning

confidence: 99%

“…112 IrtA-NTD is predicted to be a ferric-siderophore reductase. 98 Additionally, IrtA-NTD possesses a RXYS(T) motif, which has been associated with flavin adenine dinucleotide (FAD) binding in ferric oxidoreductases, Figure 8. 98 Notably, unlike IrtA, IrtB lacks an analogous domain.…”

Section: 64mentioning

confidence: 99%

“…98 Additionally, IrtA-NTD possesses a RXYS(T) motif, which has been associated with flavin adenine dinucleotide (FAD) binding in ferric oxidoreductases, Figure 8. 98 Notably, unlike IrtA, IrtB lacks an analogous domain. 97 Results from in vitro binding experiments show that IrtA-NTD binds FAD in a 1:1 ratio.…”

Section: 64mentioning

confidence: 99%

“…97 Results from in vitro binding experiments show that IrtA-NTD binds FAD in a 1:1 ratio. 98 To show that the FAD-dependent IrtA-NTD domain is required for fully functional IrtA/IrtB carboxymycobactin-dependent iron uptake, a mutational analysis of the conversed FADbinding motif (Arg70, Tyr72 and Thr73) was carried out. The results demonstrated that mutating IrtA-NTD Tyr72 and Thr73 to Ala resulted in diminished growth under low iron conditions, while the IrtA-NTD Arg70Ala mutation did not affect growth, suggesting that Tyr72 and Thr73 are critical for IrtA-NTD FAD binding and activity.…”

Section: 64mentioning

confidence: 99%

“…Taken together the results suggest that FAD binding to IrtA-NTD is required for fully functional IrtA/IrtB, and, furthermore, since IrtA-NTD is located on the cytoplasmic side of the inner membrane, IrtA-NTD is a cytoplasmic ferric-siderophore reductase. 98 In addition to IrtA-NTD, the Mtb proteome is predicted to encode another ferric-siderophore reductase, ViuB, (Figure 8), which is homologous to IrtA-NTD 98 and a ferric-siderophore reductase ViuB from Vibrio cholerea. 113 Mtb ViuB is induced under low iron conditions 110 and in bacillus Calmette-Guérin (BCG, an attenuated strain of Mycobacterium bovis) replicating in mouse macrophages.…”

Section: 64mentioning

confidence: 99%

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Iron Acquisition in Mycobacterium tuberculosis

Chao¹,

Sieminski²,

Owens

et al. 2018

Chem. Rev.

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The highly contagious disease tuberculosis (TB) is caused by the bacterium Mycobacterium tuberculosis (Mtb), which has been evolving drug resistance at an alarming rate. Like all human pathogens, Mtb requires iron for growth and virulence. Consequently, Mtb iron transport is an emerging drug target. However, the development of anti-TB drugs aimed at these metabolic pathways have been restricted by the dearth of information on Mtb iron acquisition. In this review, we describe the multiple strategies utilized by Mtb to acquire ferric iron and heme-iron. Mtb iron uptake is a complex process, requiring biosynthesis and subsequent export of Mtb siderophores, followed by ferric iron scavenging and ferric-siderophore import into Mtb. Additionally, Mtb possesses two possible heme uptake pathways, and an Mtb-specific mechanism of heme degradation that yields iron and novel heme-degradation products. We conclude with perspectives for potential therapeutics that could directly target Mtb heme and iron uptake machineries. We also highlight how hijacking Mtb heme and iron acquisition pathways for drug import may facilitate drug transport through the notoriously impregnable Mtb cell-wall.

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Section: 6mentioning

confidence: 99%

Section: 64mentioning

confidence: 99%

Section: 64mentioning

confidence: 99%

Section: 64mentioning

confidence: 99%

Section: 64mentioning

confidence: 99%

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Iron Acquisition in Mycobacterium tuberculosis

Chao¹,

Sieminski²,

Owens

et al. 2018

Chem. Rev.

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Psychological and Pedagogical Support Experience for the Formation of Students' Psychological Readiness for the Final Certification

Pikalova¹

2021

VOGU

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Today, there is a wide variety of models of psychological and pedagogical support, each of which is focused on providing assistance and support to all subjects of the educational process, taking into account the specifics of the identified problems. Purpose of the study: to substantiate the experience of psychological and pedagogical support for the formation of psychological readiness for final certification, using a theoretical analysis of scientific literature, questionnaires, testing, ascertaining and forming an experiment, qualitative and quantitative analysis of the results. I have studied the experience of preparing and implementing a program for organizing psychological and pedagogical support for the formation of psychological readiness for final certification, presented in an interactive form of group interaction. This made it possible to identify the possibilities of psychological and pedagogical support for the formation of psychological readiness of students for the final certification and to determine the form and methods of support in the form of trainings. It was revealed that for effective support of the formation of psychological readiness, conditions such as the readiness of the student to change and a favorable educational environment are necessary. The prospect of research on the formation of psychological readiness is seen in the expansion of the forms and methods of accompanying students in preparation for the final certification; creation of psychological and pedagogical conditions in the educational organization, necessary and sufficient for the effective formation of all components of psychological readiness, accompanying students.

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Pedagogy

Cited by 2 publications

References 0 publications

Iron Acquisition in Mycobacterium tuberculosis

Iron Acquisition in Mycobacterium tuberculosis

Psychological and Pedagogical Support Experience for the Formation of Students' Psychological Readiness for the Final Certification

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