Peculiarities of the secondary structure of bacteriophage DNA in situ. V. Change in DNA conformation inside the phages under the influence of formaldehyde

Tikchonenko, T.I.; Kislina, O S; Добров, Е. Н.

doi:10.1016/s0003-9861(74)80002-0

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1988

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Cited by 8 publications

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Study of the circular dichroism of bacteriophage ϕ6 and ϕ6 nucleocapsid

1986

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SynopsisWe have studied the CD of both bacteriophage 4 6 and 4 6 nucleocapsid in order to assess the in situ state of the viral, double-stranded RNA (dsRNA). The results showed that packaged (p6 RNA is slightly hyperdichroic ( 4 4 % ) at 264 nm relative to dsRNA in solution. Also, the CD of dsRNA within both the virus and the nucleocapsid was unlike CD spectra of any of the three types of dsRNA condensed in uitro, as described in the accompanying paper. Using a curve-fitting program described elsewhere (Edmondson, S.P. & Gray, D.M. (1983) Nucleic Acids Res. 11,175-192), we have fit reference CD spectra of (p6 RNA and protein to measured CD spectra of $6 intact phage and nucleocapsid. Results of the computer analysis indicated that 46 RNA in situ is spectrally similar to dsRNA that is slightly dehydrated.

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