Background: Cholangitis, or acute inflammation of the bile ducts, is associated with infection in the bile ducts during severe obstruction. Cholangitis is considered a clinical condition that requires urgent treatment. Despite a wide range of surgical methods, the search for the most effective method to treat cholangitis efficiently continues today. Against this background, studies on the portal triad structures are undoubtedly relevant.Objectives: This study aims to study the morphological changes of the biliary vascular structures of the portal triad and the hepatic veins during experimental cholangitis. Methods: Twenty-five rat models were used for experimental cholangitis. Morphological changes of the portal triad structures and hepatic veins were studied by histological (hematoxylin and eosin staining), immunohistochemical (Pan Cytokeratin AE1/AE3 staining), and histochemical (Masson's Trichrome staining) methods. Results: There are infiltration areas around the portal triad; the bile ducts are dilated, infiltrative changes are observed on the atrophied walls, and sharp sinusoidal dilatation, edema, and hemorrhage are revealed. Microthrombi and inflammatory processes with lymphocytes and neutrophils are detected in the central vein. Bacterial colonies are present in the bile ducts. Conclusions: During cholangitis, pathological processes in the liver develop very quickly. The bile is infected; due to many bacteria and a high concentration of endotoxins, inflammatory reactions develop in the sinusoids. Severe venous outflow impairment takes place.