Peculiarities of modification by astaxanthin of radiation-induced damages in the genome of human blood lymphocytes exposed in vitro on different stages of the mitotic cycle

Kurinnyi, D. A.; Rushkovsky, Stanislav; Demchenko, O. M.; Pilinska, M A

doi:10.3103/s0095452718010073

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…Therefore, it is becoming increasingly important to find safe and effective radioprotective agents with high biocompatibility with human cells. ASX, which has come to the fore in recent years, is a powerful natural antioxidant and known to be the only carotenoid that can be transported to the brain by crossing the blood–brain barrier via transcytosis (Kurinnyi, Rushkovsky, et al, 2018; Xue et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

In vivo protective efficacy of astaxanthin against ionizing radiation‐induced DNA damage

Aşcı Çelik,

Toğay

2023

Chem Biol Drug Des

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Astaxanthin, a carotenoid pigment, is believed to be effective in the repair of DNA damage. Our study evaluates the effect of astaxanthin on DNA damage in rats exposed to whole‐body radiotherapy using the comet assay. Thirty‐two male rats were randomly divided into four groups (control, ionizing radiation, astaxanthin, and radiation+astaxanthin). The radiation and radiation+astaxanthin groups were exposed to X‐rays at a dose of 8 gray (0.62 gray/min). Astaxanthin was administered at 4 mg/kg by gavage for 7 days starting from irradiation. The %TailDNA parameter was chosen as an indicator of DNA damage and the results were compared using one‐way ANOVA. %TailDNA was 3.24 ± 3.12 in the control group, 2.85 ± 2.73 in the astaxanthin group, 4.11 ± 7.90 in the radiation group, and 3.59 ± 4.05 in the radiation+astaxanthin group. There was a significant increase in DNA damage in the radiation group, compared with the control and astaxanthin groups (p < .001). DNA damage was reduced in the radiation+astaxanthin group compared with the radiation group (p < .05). Although this decrease did not reduce damage to the level of the control group, it was significant. The decrease in radiation‐induced DNA damage by astaxanthin administration in our study supports the hypothesis that astaxanthin is a promising agent for against/reducing DNA damage.

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Section: Discussionmentioning

confidence: 99%

In vivo protective efficacy of astaxanthin against ionizing radiation‐induced DNA damage

Aşcı Çelik,

Toğay

2023

Chem Biol Drug Des

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“…The results showed that astaxanthin has radioprotective activity by reducing unstable cytogenetics markers (Kurinnyi et al 2016 ). The second study used human blood lymphocytes exposed to 1 Gy of γ radiation, and found that astaxanthin at 20 µg/ml has the ability to reduce DNA damage and apoptosis level (Kurinnyia et al 2017 ). The third study was conducted on human peripheral blood lymphocytes.…”

Section: In Vitro and In Vivo Studies Based On Radiation-induced Damagementioning

confidence: 99%

Advances on marine-derived natural radioprotection compounds: historic development and future perspective

Abraham

Alghazwi

Liang

et al. 2021

Mar Life Sci Technol

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Natural extracts and compounds from marine resources have gained intensive scientific and industry attention for radioprotective activities in the past ten years. However, the marine-derived radioprotectants have been studied against UV-rays, gamma (γ)-rays and X-rays for more than 30 years. This review aims to identify key marine-derived extracts/compounds and their modes of action studied for radioprotective activities from 1986 to 2019. A comprehensive survey was conducted to establish the trend in terms of the publications each year and the countries of origin. A total of 40 extracts and 34 natural compounds showing radioprotective activities against UV-rays, gamma (γ)-rays and X-rays were identified from a range of marine plants and animals. These extracts and compounds are broadly categorized into polysaccharides, phlorotannins, carotenoids and mycosporine-like amino acids (MAAs). Macroalgae and microalgae were found to be the dominant sources of polysaccharides, phlorotannins and carotenoids. MAAs were mainly identified in algae, sponges, sea cucumber and corals that showed significant UV-absorbing activities. A number of radioprotective mechanisms were shown by these compounds, predominantly free radicals scavenging, inhibition of apoptosis, UV-ray absorption and DNA damage-repair signaling pathways. While these bio-discoveries warrant further investigation and development of radioprotective therapeutics, however, the lack of clinical studies is a major obstacle to be tackled in the future.

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“…The decrease in the intensity of radiation-induced genome damages on the chromosomal and molecular levels was selected as an indicator of radioprotective effect of astaxanthin. The studies were conducted using a combination of the methods of classical cytogenetic analysis (G 0 -radiation sensitivity assay and G 2 -radiation sensitivity assay) and the method of single-cell electrophoresis (comet assay) [25][26][27][28][29].…”

Section: Investigation Of Radioprotective Properties Of Astaxanthinmentioning

confidence: 99%

“…Irr, irradiated in dose 1.0 Gy cultures; Irr + A, irradiated and supplemented with 20.0 μg/ml astaxanthin cultures. In one to ten groups of "comets," 10% (bold line) is the control value for all groups [28].…”

Section: Progress In Carotenoid Researchmentioning

confidence: 99%

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Astaxanthin as a Modifier of Genome Instability after γ-Radiation

Kurinnyi¹,

Rushkovsky²,

Demchenko³

et al. 2018

Progress in Carotenoid Research

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The chapter is devoted to study the effects of astaxanthin on the frequency of chromosomal aberrations and the level of DNA damages in human peripheral blood lymphocytes under ionizing radiation exposure in vitro. To achieve the purpose of the research, a combination of classical cytogenetic methods (G 0-and G 2-radiation sensitivity assays) and method of single-cell electrophoresis (comet assay) was used. The specificity of the modifying effect of astaxanthin on radiation-induced genomic injuries depending on the stage of the cell cycle had been determined. Significant weakening of the negative effect of ionizing radiation on the G 0 stage and the absence of a radioprotective effect on the S and G 2 stages of the cell cycle may be associated with activation by astaxanthin of apoptosis in irradiated cells with a critically high level of the genome damages. The research results not only testify about strong radioprotective effect of astaxanthin but also demonstrate the feasibility of the parallel use of cytogenetic and molecular genetic methods to assess the impact as mutagens as well as factors that modify the effect of mutagens on genome stability.

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Peculiarities of modification by astaxanthin of radiation-induced damages in the genome of human blood lymphocytes exposed in vitro on different stages of the mitotic cycle

Cited by 11 publications

References 23 publications

In vivo protective efficacy of astaxanthin against ionizing radiation‐induced DNA damage

In vivo protective efficacy of astaxanthin against ionizing radiation‐induced DNA damage

Advances on marine-derived natural radioprotection compounds: historic development and future perspective

Astaxanthin as a Modifier of Genome Instability after γ-Radiation

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