Peculiarities of Microscopic Changes in the Tissue of the Human Organism at Different Periods of Death

Sokol, V. K.

doi:10.24061/2707-8728.1.2022.4

Cited by 3 publications

(7 citation statements)

References 5 publications

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“…Savka et al (2021), V.K. Sokol (2022) note that the vast majority of these methods were invented by scientists in the field of forensic medicine. These methods are primarily intended for the study of objects such as human corpses.…”

Section: Literature Reviewmentioning

confidence: 99%

Determination of the time of death of a domestic cat by measuring the area of a wet spot

Shkundia,

Serdioucov

2024

Ukrainian journal of veterinary sciences

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Animal cruelty is a criminal offence under the current legislation of Ukraine. When investigating criminal proceedings related to animal cruelty, including domestic cats, it is often necessary to establish the time of death. Therefore, the development of new methods for determining it and improving existing ones is extremely relevant. The purpose of the study was to establish the relationship between the obtained values of wet spot area indicators and the time of death. To achieve this goal, the authors propose a new method for determining the age of death of domestic cats based on the results of measuring the area of a wet spot obtained from skeletal muscle tissue samples of corpses (Shkundia method). To do this, samples of muscle tissue were taken from the corpses of cats, a wet spot preparation was obtained by pressing and its area was calculated to establish a correlation between the values of this indicator and the time that has passed since death. The study of the obtained indicators established that these values are constant, and the features of fluctuations in the values of this indicator with the time elapsed since the death of the animals were established and shown graphically. Using a number of statistical methods, the absence of dependence of wet spot area indicators on the muscles from which samples were taken for research and animal breeds was determined. The nature of changes in the values of the wet spot area indicator depending on time is established and the features of their fluctuations in different periods during 27 days from the moment of animal death were explained. Reference values of these indicators depending on the time elapsed since the death of animals are presented, which can be used by forensic veterinary experts in practical work to determine the time of the occurrence of death of domestic cats. Due to a number of advantages, this method can be widely implemented in the practice of forensic veterinary medicine

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Section: Literature Reviewmentioning

confidence: 99%

Determination of the time of death of a domestic cat by measuring the area of a wet spot

Shkundia,

Serdioucov

2024

Ukrainian journal of veterinary sciences

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“…A method for determining the time of death based on the results of microscopic examination, as reported by V.K. Sokol (2022), is based on visual and morphometric assessment of tissue-level changes caused by tertiary cadaveric changes, namely autolysis and putrefaction. The development of destructive changes directly depends on the time of death.…”

Section: Literature Reviewmentioning

confidence: 99%

“…I.G Savka et al (2021),. V.K Sokol (2022). note that the vast majority of these methods were invented by scientists in the field of forensic medicine.…”

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confidence: 99%

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2024

Ukrainian journal of veterinary sciences

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“…A large number of methods have been developed for identifying time of death (Hryhorian, 2019;Voronov, 2022). We should note that most of them have been designed for the sphere of forensic expertise of human corpses (Sarkisova, 2021;Savka et al, 2021;Davydenko, 2022;Lytvynenko, 2022;Sokol, 2022;Voronov, 2022), and only a small proportion of them are used to examine animal corpses (Sapienza et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…A separate mention should be made for identification of time of death according to the results of histological studies of the tissue samples from a corpse, used in both forensic medicine and forensic veterinary expertise (Ceciliason et al, 2021;Delgado et al, 2021;Saber et al, 2021;Sokol, 2022). The method consists in evaluation of autolysis-and decay-related (breakdown changes) changes in the tissues.…”

Section: Introductionmentioning

confidence: 99%

Identification of time of death of cats according to histological changes in some organs

Serdioucov,

Shkundia,

Kruchynenko

2023

Regul. Mech. Biosyst.

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Determining the time of an animal’s death is an extremely relevant subject in the practice of modern forensic medicine, because the issue often arises when investigating crimes related to animal abuse, in particular, with domestic cats Felis silvestris catus (Linnaeus, 1758). Methods of identifying time of cats’ death are currently developed insufficiently and their introduction into the veterinary-forensic practice has been limited, including the histological method we have employed. We used five corpses of cats, from which we periodically, after certain time periods, gathered the material for histological study, sampling such organs as the liver, kidneys, lungs, heart (myocardium), and the skeletal muscles. From the collected material, we made histological sections and stained them with hematoxylin and eosin. We determined the general patterns in changes occurring in the internal organs of cats over time, namely changes in the staining intensity of the cell elements and intercellular structures on the histopreparations; formation of detritus-filled cavities; a number of distinctive changes in the connective-tissue stroma of the organs; emergence of baciliform bacteria in the tissues and formation of their colonies. In the muscle tissue, we found specific features such as loss of alignment and emergence of transversal grooves with subsequent fragmentation. The study revealed that the development of those changes in each organ clearly correlated with time that has passed since each animals had died. Thus, a complex evaluation of detected changes can give a more accurate – compared with other existing methods – assessment of the time of death of a corpse submitted to forensic veterinary examination. We recommend using the histological method for animals presumed to have been dead for no more than 18 days, because later the tissue disintegration reaches such a level that complicates the correlation of changes with time. We consider it promising to study the time of death of domestic cats using other methods for identifying criteria for postmortem intervals longer than 18 days, and also identifying time of death in other species of animals.

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Peculiarities of Microscopic Changes in the Tissue of the Human Organism at Different Periods of Death

Cited by 3 publications

References 5 publications

Determination of the time of death of a domestic cat by measuring the area of a wet spot

Determination of the time of death of a domestic cat by measuring the area of a wet spot

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Identification of time of death of cats according to histological changes in some organs

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